KIFC1 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA057545A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: K562 whole cell lysate(30µg), NIH/3T3 whole cell lysate(30µg), MCF-7 whole cell lysate(30µg) All lanes: KIFC1 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 74 kDa Observed band size: 74 kDa Exposure time：3min IHC image of CSB-RA057545A0HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Overlay Peak curve showing jurkat cells stained with CSB-RA057545A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Target Background

Product Details

Uniprot No.

Q9BW19

Target Names

KIFC1

Alternative Names

Kinesin-like protein KIFC1 (Kinesin-like protein 2) (Kinesin-related protein HSET), KIFC1, HSET KNSL2

Species Reactivity

Human

Immunogen

Recombinant Antibody

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

1F9

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

KIFC1, also known as HSET, is a minus-end-directed kinesin motor protein that plays a critical role in spindle assembly and chromosome segregation during cell division. Its involvement in centrosome clustering makes it particularly significant in cancer research, as tumor cells with supernumerary centrosomes depend on KIFC1 activity to achieve bipolar division and avoid mitotic catastrophe. This dependency has positioned KIFC1 as both a prognostic marker and a potential therapeutic target across multiple cancer types.

This recombinant monoclonal antibody, clone 1F9, offers the consistency and reproducibility that demanding research applications require. Produced using recombinant technology with a defined sequence, it eliminates the lot-to-lot variability inherent in traditional hybridoma-derived antibodies, ensuring your experimental results remain comparable across extended studies.

Validation testing demonstrates reliable performance across multiple platforms. Western blot analysis detects KIFC1 at the expected 74 kDa molecular weight in human K562 leukemia cells and MCF-7 breast cancer cells, as well as mouse NIH/3T3 fibroblasts, confirming specificity for the target protein. Immunohistochemistry staining in paraffin-embedded human testis tissue reveals clear localization patterns at 1:100 dilution using standard citrate buffer antigen retrieval. Flow cytometry analysis in Jurkat cells shows distinct positive signal separation from isotype controls, enabling quantitative assessment of KIFC1 expression at the single-cell level.

The flexibility to move between tissue sections, cell lysates, and suspension cell analysis makes this antibody well-suited for researchers investigating mitotic regulation, centrosome biology, or cancer cell vulnerabilities. Whether characterizing KIFC1 expression in tumor samples or validating functional studies, this reagent provides the technical foundation for reproducible results.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Minus end-directed microtubule-dependent motor required for bipolar spindle formation. May contribute to movement of early endocytic vesicles. Regulates cilium formation and structure.

Gene References into Functions

Data show that kinesin-related protein HSET (HSET) is incapable of forming asters from preformed, nongrowing microtubule (non-MT) MTs, but rapidly forms MT asters in the presence of soluble (non-MT) tubulin. PMID: 29985404
KIFC1 interacts with F508del-CFTR. PMID: 30066085
nuclear KIFC1 (nKIFC1) correlated significantly with Ki67 in White triple-negative breast cancer (TNBCs) but not in African American (AA) TNBCs, suggesting that nKIFC1 is not merely a surrogate for proliferation in AA TNBCs. PMID: 28218233
Results identified Stathmin as a direct target of KIFC1. PMID: 28885720
The overexpression of KIFC1 was correlated closely with the progression of hepatocellular carcinoma (HCC) and poor prognosis, and suggested that the expression levels of KIFC1 are a potential prognostic biomarker for HCC. PMID: 29620256
Circulating pri-miRNA-944 and 3662 can improve non-invasive potential non-small cell lung cancer detection of operable stages of SCC and AC PMID: 28964573
These findings reveal a new stationary binding model that kinesin-14 KIFC1 proteins function as crosslinkers between the Golgi apparatus and the microtubules and contribute to the central positioning and structural maintenance of the Golgi apparatus. PMID: 28430595
Taken together, these results suggest that up-regulation of KIFC1 may induce docetaxel resistance. PMID: 27665358
Low post translational modifications of KIFC1 protein is associated with neoplasms. PMID: 28209915
KIFC1 is overexpressed in GC. PMID: 27176706
Association of KIFC1 gene expression and centrosome amplification with high-grade serous ovarian carcinoma. PMID: 26992853
stabilization of the centrosome-spindle pole interface by the CEP215-HSET complex could promote survival of cancer cells containing supernumerary centrosomes. PMID: 26987684
KIFC1 silencing significantly reduces breast cancer cell viability. PMID: 26177331
Data provides the first evidence of centrosome clustering-independent activities of HSET that fuel tumor progression. PMID: 25788277
We show that some are new substrates of the anaphase-promoting complex/cyclosome and validate KIFC1 and RacGAP1/Cyk4 as two such targets involved respectively in timely mitotic spindle disassembly and cell spreading PMID: 24857844
Our data underscore KIFC1 as a putative biomarker that predicts worse prognosis, poor overall survival and may serve as a potential marker of onset of metastatic dissemination in ovarian cancer patients. PMID: 25028599
Study demonstrates microtubule tip-tracking of human kinesin-14 HSET and show that EB1 is sufficient to induce this dynamic microtubule end localization. PMID: 24039245
Acentrosomal spindle assembly mechanisms are hyperactive in cancer cells and promote HSET. PMID: 22946058
we suggest that KIFC1 plays an essential role for bipolar MTOC formation and maintaining chromosomal stability in the mitosis . PMID: 23318213
A case-control analysis was carried out by comparing the genotype distribution of six KIFC1 single-nucleotide polymorphisms between 93 aspirin exacerbated respiratory disease cases and 96 aspirin-tolerant asthma controls in a Korean population PMID: 22201025
The intense placental expression of KIFC1 in syncytiotrophoblast and KIF17 in vascular endothelium suggests that both proteins might be important in a cargo-transport system. KIFC1 and KIF17 expression are increased of both in preeclamptia and diabetes. PMID: 19679349
An analysis was made of the weak interactions of ADP-Unc104 and ADP-kinesin with microtubules and their inhibition by MAP2. PMID: 17326138
HSET has a role in controlling spindle length by cross-linking and sliding microtubules. PMID: 19116309
High KIFC1 expression is associated with metastatic spread to the brain in primary non-small cell lung cancer. PMID: 19190132
Chromosome congression in HSET + hNuf2 co-depleted cells required the plus-end directed motor CENP-E , which has been implicated in the gliding of mono-oriented kinetochores alongside adjacent K-fibres. PMID: 19525938

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Subcellular Location

Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle. Early endosome.

Protein Families

TRAFAC class myosin-kinesin ATPase superfamily, Kinesin family, NCD subfamily

Database Links

HGNC: 6389

OMIM: 603763

KEGG: hsa:3833

STRING: 9606.ENSP00000393963

UniGene: Hs.436912

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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