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  • Peptide

KLC3 Recombinant Monoclonal Antibody

Datasheet
Code CSB-RA889602A0HU
Size US$210
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Image
  • Western Blot
    Positive WB detected in: HepG2 whole cell lysate(20µg),COLO-205 whole cell lysate(20µg),Jurkat whole cell lysate(20µg), A549 whole cell lysate(20µg),Hela whole cell lysate(20µg),MCF-7 whole cell lysate(20µg),Mouse skin tissue lysate(20µg)
    All lanes: Kinesin light chain 3 antibody at 1:1000
    Secondary
    Goat polyclonal to rabbit IgG at 1/40000 dilution
    Predicted band size: 55 kDa
    Observed band size: 57 kDa
    Exposure time：2s
  • IHC image of CSB-RA889602A0HU diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
  • IHC image of CSB-RA889602A0HU diluted at 1:100 and staining in paraffin-embedded human skin tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
  • Overlay Peak curve showing Hela cells stained with CSB-RA889602A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Product Details

Uniprot No.
Q6P597
Target Names
KLC3
Alternative Names
Kinesin light chain 2 antibody; Kinesin light chain 3 antibody; KLC2 antibody; KLC2 like antibody; KLC2-like antibody; KLC2L antibody; KLC3 antibody; KLC3_HUMAN antibody; KLCt antibody; KNS2B antibody
Species Reactivity
Human, Mouse
Immunogen
A synthesized peptide derived from human KLC3
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone No.
14D4
Purification Method
Affinity-chromatography
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Form
Liquid
Tested Applications
ELISA, WB, IHC, FC
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:2000
IHC 1:50-1:200
FC 1:50-1:200
Protocols
ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol
Troubleshooting and FAQs
Antibody FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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KLC3 Proteins

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Target Background

Function
Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport.
Gene References into Functions
  1. Microtubule-bound kinesin-1 and kinesin-3 motor domains were visualized at multiple steps in their ATPase cycles--including their nucleotide-free states--at approximately 7 A resolution using cryo-electron microscopy. PMID: 25209998
  2. Rat KLC3 interacts with KHC and microtubules in vitro. Expressed in spleen, intestine, brain, ovary, testis. Testis expression restricted to post-meiotic spermatids. PMID: 11319135
  3. Rat KLC3 associates with the outer dense fibers of maturing sperm tails. PMID: 12594206
  4. Rat KHC3 associates with mitochondria in sperm tails PMID: 15464570
Subcellular Location
Cytoplasm, cytoskeleton.
Protein Families
Kinesin light chain family
Database Links

HGNC: 20717

OMIM: 601334

KEGG: hsa:147700

STRING: 9606.ENSP00000375810

UniGene: Hs.298079

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