KLC3 Recombinant Monoclonal Antibody

Kinesin light chain 3 (KLC3) serves as a critical component of the kinesin motor complex, facilitating the transport of cellular cargo along microtubules. This protein plays essential roles in intracellular trafficking processes, making it a valuable target for researchers investigating cytoskeletal dynamics, organelle transport, and cellular organization mechanisms.

This recombinant monoclonal antibody, clone 14D4, offers the consistency and reliability that demanding experimental workflows require. Developed using recombinant technology with a defined sequence, this antibody eliminates the lot-to-lot variability that can compromise longitudinal studies and reproducibility. The rabbit host-derived IgG format provides excellent signal-to-noise characteristics across multiple detection platforms.

Validation data demonstrates robust performance across diverse experimental approaches. Western blot analysis reveals specific detection of KLC3 at approximately 57 kDa across an extensive panel of human cell lines including HepG2, COLO-205, Jurkat, A549, HeLa, and MCF-7, as well as mouse skin tissue. The slightly higher observed molecular weight compared to the 55 kDa predicted size likely reflects post-translational modifications such as glycosylation. Immunohistochemistry applications have been validated in paraffin-embedded human cervical cancer and skin tissue sections using citrate buffer antigen retrieval, producing clear visualization of target expression patterns. Flow cytometry analysis in HeLa cells confirms utility for single-cell studies of KLC3 expression.

With confirmed cross-species reactivity in human and mouse samples, this antibody supports comparative studies across model systems. Researchers investigating motor protein function, neurodegenerative disease mechanisms, or cellular transport pathways will find this antibody a dependable tool for their investigations.