Product Details

Uniprot No.

Target Names

KLRG1

Alternative Names

Killer cell lectin-like receptor subfamily G member 1 (C-type lectin domain family 15 member A) (ITIM-containing receptor MAFA-L) (MAFA-like receptor) (Mast cell function-associated antigen), KLRG1, CLEC15A MAFA MAFAL

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human KLRG1

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

8E11

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, FC

Recommended Dilution

Application	Recommended Dilution
FC	1:50-1:200

Protocols

ELISA Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

To create the KLRG1 recombinant monoclonal antibody, several steps are involved. The KLRG1 monoclonal antibody is first harvested and its gene sequence is determined. A vector carrying the KLRG1 monoclonal antibody gene is then constructed and transfected into a host cell line for culture. During the production of the KLRG1 monoclonal antibody, a synthesized peptide from human KLRG1 acts as the immunogen. Affinity chromatography is used to purify the KLRG1 recombinant monoclonal antibody, which is then evaluated for specificity through ELISA and FC applications.

KLRG1 is a type II transmembrane protein that belongs to the C-type lectin superfamily. It is mainly expressed on the surface of natural killer (NK) cells, T cells, and some memory B cells. KLRG1 is involved in regulating the immune response by inhibiting the activation of NK cells and T cells. Specifically, KLRG1 acts as an inhibitory receptor on the surface of NK cells and T cells, and its engagement with its ligand, E-cadherin, leads to the downregulation of cell-mediated cytotoxicity and cytokine production. KLRG1 has also been shown to be involved in regulating the differentiation and function of memory T cells. In addition to its role in the immune response, KLRG1 has been implicated in other physiological processes, such as wound healing and tissue repair.

Target Background

Function

Plays an inhibitory role on natural killer (NK) cells and T-cell functions upon binding to their non-MHC ligands. May mediate missing self recognition by binding to a highly conserved site on classical cadherins, enabling it to monitor expression of E-cadherin/CDH1, N-cadherin/CDH2 and R-cadherin/CDH4 on target cells.

Gene References into Functions

KLRG1 might contribute to the impaired antitumor immunity of memory T cells in tumor microenvironment PMID: 27557510
Killer Cell Lectin-like Receptor G1 Inhibits NK Cell Function through Activation of Adenosine 5'-Monophosphate-Activated Protein Kinase PMID: 27566818
The results suggest that a disease-associated SNP located within the 3'UTR of KLRG1 directly interferes with miR-584-5p binding, allowing for KLRG1 mRNA differential accumulation, which in turn may contribute to pathogenesis of pemphigus. PMID: 27424220
Demonstrate the presence of increased KLRG1-expressing T-cells in tuberculosis-treated individuals, and present KLRG1 as a marker of decreased human T-cell proliferation following BCG-vaccination. PMID: 26750180
This finding provides a rationale for the reciprocal expression of KLRG1 and CD103 in different CD8(+) T-cell subsets. PMID: 26014037
high levels of TfRs such as those found on activated lymphocytes were found to be associated with decreased KLRG1 inhibitory function, indicating that TfRs may sequester KLRG1 from interacting with cadherins. PMID: 24752778
More T cells were KLRG1+ in the synovial fluid of patients with spondylarthritis/rheumatoid arthritis than crystal arthritis or controls. Those T cells were more functionally active, and migrated towards the synovial fluid of SpA/RA patients. PMID: 23740233
KLRG1 was overexpressed on CD4(+) T cells. PMID: 24337749
Data indicate that increments of CD8 + effector memory T cells in human and mouse chronic lymphocytic leukemia (CLL)(Emu-TCL1 model) were due to an expansion of the inhibitory killer cell lectin-like receptor G1 (KLRG1) expressing cellular subset. PMID: 24022692
These results indicate that KLRG1 negatively regulates natural killer cell numbers and functions via the Akt pathway, thus providing a novel marker and therapeutic target for hepatitis C virus infection. PMID: 23966413
The lower inhibitory capacity of mKLRG1 compared with hKLRG1 can thus be rationalized by a decreased proportion of dimeric entities, which can be pinpointed to a single amino acid. PMID: 22684915
cytomegalovirus positive had lower proportions of NK-cells expressing inhibitory receptors (KLRG1 and CD158a) PMID: 21933704
trastuzumab-mediated antibody-dependent cellular cytotoxicity was markedly enhanced by KLRG1-negative peripheral blood mononuclear cells PMID: 21387286
Results demonstrated a substantial reduction in both CD94- and KLRG1- expressing CD3(-)CD56(+) NK cells with increased age. PMID: 20394788
Expression of MafA analyzed in conditional knockout mice during embryonic development is the first specific marker of a subpopulation of "early c-ret" positive sensory neurons. PMID: 20213756
human effector and memory T cells expressing KLRG1 lack proliferative capacity PMID: 12393723
KLRG1 expression discriminated between cord blood T cells that differed in their post-thymic expansion rate PMID: 15368283
KLRG1 is expressed in the overwhelming majority of CD8-positive T cells specific for epitopes of cytomegalovirus or Epstein- Barr virus during the latent stage; a slightly lower KLRG1 expression level is observed in HIV-specific T8 cells. PMID: 15879103
virus-specific CD8+ T cells are mostly KLRG1+ in chronic viral infections (human immunodeficiency virus, cytomegalovirus, and Epstein-Barr virus) but not in resolved infection (influenza virus) PMID: 16140789
peripheral HCV-specific CD8+ T cells were CD127+ and KLRG1-; Intrahepatic virus-specific CD8+ T cells displayed significantly reduced levels of CD127 expression but similar levels of KLRG1 expression compared to the peripheral blood. PMID: 17079288
study demonstrates that E-cadherin represents a ligand for KLRG1 and that ligation of KLRG1 by E-cadherin inhibited effector cell functions of polyclonal NK cells PMID: 17617594
Certain aspects of the dysfunction seen in highly differentiated CD8+ T cells are maintained actively by KLRG1 signaling. KLRG1 blockade enhances Akt (ser(473)) phosphorylation & T-cell-receptor-induced proliferation of CD8+CD28-CD27- T cells. PMID: 19406987
E-cadherin may coengage KLRG1 and alpha(E)beta(7) and that KLRG1 overcomes its exceptionally weak affinity for cadherins through multipoint attachment to target cells, resulting in inhibitory signaling. PMID: 19604491

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Subcellular Location

Cell membrane; Single-pass type II membrane protein.

Tissue Specificity

Expressed specifically on natural killer (NK) cells and T-cells, mainly CD8 T-cells.

Database Links

HGNC: 6380

OMIM: 604874

KEGG: hsa:10219

STRING: 9606.ENSP00000349477

UniGene: Hs.558446

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Code	CSB-RA233042A0HU
Size	US$210
	Order now
Image	Overlay Peak curve showing A549 cells surface stained with CSB-RA233042A0HU (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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KLRG1 Recombinant Monoclonal Antibody

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