Product Details

Uniprot No.

P35900

Target Names

KRT20

Alternative Names

Keratin, type I cytoskeletal 20 (Cytokeratin-20) (CK-20) (Keratin-20) (K20) (Protein IT), KRT20

Species Reactivity

Human

Immunogen

A synthesized peptide derived from Human KRT20

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

17A11

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IHC, FC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

In the production of the KRT20 recombinant monoclonal antibody, in vitro expression systems are employed, involving the cloning of KRT20 antibody DNA sequences from immunoreactive rabbits. The immunogen used is a synthesized peptide derived from the human KRT20 protein. Subsequently, the genes encoding the KRT201 antibodies are inserted into plasmid vectors, and these recombinant plasmid vectors are then transfected into host cells to enable antibody expression. Post-expression, the KRT20 recombinant monoclonal antibody undergoes purification through affinity chromatography. It is thoroughly tested for functionality in ELISA, IHC, and FC applications, demonstrating reactivity with the human KRT20 protein during these evaluations.

The main role of KRT20 protein is to provide structural support and integrity to epithelial tissues, particularly in the gastrointestinal tract. KRT20 also serves as a tissue-specific marker and can be involved in diagnostic applications, especially in the context of certain types of cancers and pathological conditions affecting epithelial tissues.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Code	CSB-RA176322A0HU
Size	US$210
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Image	IHC image of CSB-RA176322A0HU diluted at 1:50 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.61% DAB. Overlay Peak curve showing MCF-7 cells stained with CSB-RA176322A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1µg/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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KRT20 Recombinant Monoclonal Antibody

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