Product Details

Uniprot No.

P35900

Target Names

KRT20

Alternative Names

Keratin, type I cytoskeletal 20 (Cytokeratin-20) (CK-20) (Keratin-20) (K20) (Protein IT), KRT20

Species Reactivity

Human

Immunogen

A synthesized peptide derived from Human KRT20

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

17A11

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IHC, FC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Keratin 20 serves as a highly specific marker of intestinal and gastric epithelial differentiation, making it an invaluable tool for researchers investigating gastrointestinal biology and cancer pathology. Unlike other cytokeratins with broader tissue distribution, KRT20 expression is remarkably restricted to mature enterocytes, goblet cells, and Merkel cells, providing exceptional specificity when characterizing tissue origin or tracking epithelial differentiation states. This restricted expression pattern has established KRT20 as a cornerstone marker in colorectal cancer research and metastatic tumor identification.

This recombinant monoclonal antibody, generated against a synthetic peptide derived from human KRT20, offers the reproducibility that demanding research protocols require. Because the antibody sequence is defined and production occurs in controlled recombinant systems, you can expect consistent performance across experiments and between lot numbers, eliminating the variability that can complicate long-term studies or multi-site collaborations.

Validation studies demonstrate reliable performance across complementary detection platforms. Immunohistochemical staining of paraffin-embedded human small intestine tissue at dilutions between 1:50 and 1:200 reveals the expected epithelial localization pattern, with antigen retrieval performed using citrate buffer under high pressure conditions. Flow cytometry analysis using permeabilized MCF-7 cells confirms intracellular detection capability, showing clear signal separation from isotype control when used at 1:50 dilution. This application flexibility allows you to move between tissue-based and cell-based analyses using a single, well-characterized reagent.

Whether you are investigating colorectal carcinoma differentiation, mapping epithelial lineage commitment, or exploring signal transduction pathways in gastrointestinal contexts, this antibody provides the specificity and consistency your research demands.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Code	CSB-RA176322A0HU
Size	US$210
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Image	IHC image of CSB-RA176322A0HU diluted at 1:50 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.61% DAB. Overlay Peak curve showing MCF-7 cells stained with CSB-RA176322A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1µg/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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KRT20 Recombinant Monoclonal Antibody

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