Product Details

Uniprot No.

Target Names

LIG4

Alternative Names

DNA ligase IV ATP dependent antibody; DNA joinase antibody; DNA ligase 4 antibody; DNA ligase IV antibody; DNA repair enzyme antibody; DNLI4_HUMAN antibody; LIG 4 antibody; LIG4 antibody; LIG4S antibody; Ligase IV antibody; Ligase IV DNA ATP dependent antibody; Polydeoxyribonucleotide synthase [ATP] 4 antibody; Polydeoxyribonucleotide synthase 4 antibody; Polydeoxyribonucleotide synthase antibody; Polynucleotide ligase antibody; Sealase antibody

Species Reactivity

Human

Immunogen

A synthesized peptide from human LIG4 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

6E2

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

DNA ligase IV plays an essential role in the non-homologous end joining pathway, the primary mechanism by which mammalian cells repair double-strand DNA breaks. This enzyme works in concert with XRCC4 to rejoin broken DNA ends, making it a critical factor in maintaining genomic stability, V(D)J recombination during immune cell development, and cellular responses to genotoxic stress. Mutations in LIG4 are associated with LIG4 syndrome and increased sensitivity to ionizing radiation, underscoring its importance in DNA damage research and cancer biology.

This recombinant monoclonal antibody, generated from clone 6E2 in rabbit host, offers the reproducibility and consistency that demanding experimental workflows require. Because the antibody sequence is defined and production occurs in controlled recombinant systems, researchers can expect reliable performance across experiments and over time, eliminating the lot-to-lot variability that can complicate long-term studies.

Validation across multiple platforms demonstrates the versatility of this antibody. Western blot analysis detects a band at the predicted molecular weight of 104 kDa across a diverse panel of human cell lines, including HeLa, MCF-7, A431, THP-1, U-251MG, SH-SY5Y, and Jurkat cells, confirming robust detection in epithelial, monocytic, glioblastoma, and lymphocyte models. Immunohistochemistry staining in paraffin-embedded human thyroid tissue reveals clear target localization, while flow cytometry analysis in Jurkat cells demonstrates suitability for single-cell quantification studies.

Whether investigating DNA repair mechanisms, characterizing responses to DNA-damaging agents, or exploring epigenetic and nuclear signaling pathways, this antibody provides a dependable tool for researchers studying genomic integrity and its implications in disease.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Related Products

Target Background

Function

DNA ligase involved in DNA non-homologous end joining (NHEJ); required for double-strand break (DSB) repair and V(D)J recombination. Catalyzes the NHEJ ligation step of the broken DNA during DSB repair by resealing the DNA breaks after the gap filling is completed. Joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. LIG4 is mechanistically flexible: it can ligate nicks as well as compatible DNA overhangs alone, while in the presence of XRCC4, it can ligate ends with 2-nucleotides (nt) microhomology and 1-nt gaps. Forms a subcomplex with XRCC4; the LIG4-XRCC4 subcomplex is responsible for the NHEJ ligation step and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends. LIG4 regulates nuclear localization of XRCC4.

Gene References into Functions

Cells doubly deficient in Pol theta; and Lig4 exhibit 100% gene-targeting efficiency because of virtually no random integration events. PMID: 28695890
These studies provide a scaffold for defining impacts of LigIV catalytic core mutations and deficiencies in human LIG4 syndrome. PMID: 29980672
study demonstrated that there was an association between DNA ligase 4 Thr9Ile polymorphism and male infertility and suggests CT genotype as a risk factor for male infertility PMID: 28991497
An increased frequency of binucleated lymphocytes with micronuclei was increased in carriers of the T/T genotype of the LIG4 (rs1805388) gene compared to miners harbouring the C/T genotype. PMID: 28992182
Cellular NHEJ of diverse ends thus identifies the steps necessary for repair through LIG4-mediated sensing of differences in end structure and consequent dynamic remodeling of aligned ends. PMID: 28930678
This study shown that both ligase IV and XRCC4 may act in concert to modulate the development of glioma. PMID: 27508978
We demonstrated an association between six previously published single nucleotide polymorphisms (rs15869 [ BRCA2], rs1805389 [ LIG4], rs8079544 [ TP53], rs25489 [ XRCC1], rs1673041 [ POLD1], and rs11615 [ ERCC1]) and subsequent CNS tumors in survivors of childhood cancer treated by radiation therapy. PMID: 28976792
In a recombinant PNKP-XRCC4-LigIV complex, both the PNKP FHA and catalytic domains contact the XRCC4 coiled-coil and LigIV BRCT repeats. Multipoint contacts between PNKP and XRCC4-LigIV regulate PNKP recruitment and activity within NHEJ. PMID: 28453785
Data suggest that stimulation of Artemis nuclease/DCLRE1C activity by XRCC4-DNA ligase IV hetero-complex and efficiency of blunt-end ligation are determined by structural configurations at the DNA ends. (XRCC4 = X-ray repair cross complementing 4) PMID: 28696258
found that the rs228593, rs2267437 and rs1805388 functional polymorphisms probably alter the level of expression of the ATM, XRCC6 and LIG4 genes, respectively, being important in the maintenance of genomic instability in MDS PMID: 27497341
marked overlap of dyskeratosis congenita with four other genetic syndromes, confounding accurate diagnosis and subsequent management. Patients with clinical features of dyskeratosis congenita need to have genetic analysis of USB1, LIG4 and GRHL2 in addition to the classical dyskeratosis congenita genes and telomere length measurements. PMID: 27612988
The rs1805388 in LIG4 was associated with increased radioresistance. PMID: 26974709
LIG4 is highly upregulated in human colorectal cancer cells. PMID: 27009971
Five novel mutations in LIG4 and a potential hotspot mutation (c.833G > T; p.R278L) in the Chinese population with LIG4 deficiency syndrome were identified. PMID: 26762768
Our study identifies LIG4 as a predictor of an increased risk for early biochemical recurrence in prostate cancer PMID: 26134445
The genetic polymorphisms in LIG4 rs1805388 and HSPB1 rs2868371 were not obviously correlated with the risk of radiation pneumonitis and radiation-induced lung injury of lung cancer. PMID: 25811031
our results suggest that LIG4 rs10131 polymorphism in the DNA repair pathways plays an important role in the risk of glioma in a Chinese population. PMID: 25973104
Our data showed that Cd altered the phosphorylation of DNA-PKcs, and reduced the expression of both XRCC4 and Ligase IV in irradiated cells. PMID: 26201248
despite several limitations, this meta-analysis suggested that LIG4 T9I genetic variant is associated with a decreased risk of cancer among Caucasians, however, the rs1805386 gene polymorphism is not a risk factor of cancer. PMID: 25314918
High LIG4 expression is associated with less radiosensitivity of nasopharyngeal cancer. PMID: 25605214
DNA Ligase IV is required for efficient localization of XRCC4 and XLF into cell nucleus. PMID: 24984242
Ku-independent, LIGIV-dependent repair pathway exists in human somatic cells. PMID: 24837021
LIG4 Thr9Ile polymorphism is associated with treatment response in advanced non-small cell lung cancer. PMID: 24722796
Our data did not show association between LIG4 and RAD52 SNPs and SLE, its clinical manifestations or ethnicity in the tested population. PMID: 24415301
Loss of LIG4 is associated with colorectal cancer. PMID: 24282031
Results show that in cells deficient for Lig4, chromosomal translocations junctions had significantly longer deletions and more microhomologies. PMID: 25201414
Characterization of the protein interaction domains that modulate the XRCC4/Ligase IV interaction. PMID: 23794378
study reports the identification of biallelic truncating LIG4 mutations in 11 patients with microcephalic primordial dwarfism presenting with restricted prenatal growth and extreme postnatal global growth failure PMID: 24123394
DNA ligase IV expression of K562/DNR was also suppressed significantly with Sp1 family protein inhibition PMID: 24530422
DNA ligase IV and Artemis act cooperatively to promote nonhomologous end-joining PMID: 23967291
These results indicate for the first time that LIG4 rs1805388 and X-ray Repair Cross Complementing-4 (XRCC4) rs1805377, alone or in combination, are associated with a risk of gliomas. PMID: 23663450
The chromatin binding of XRCC4 was dependent on the presence of LIG4. PMID: 23994631
genetic association studies in population in China: Data suggest that SNPs in LIG4 (rs1805388, exon 2 54C>T, Thr9Ile) and RAG1 (recombination activating gene 1, rs2227973, A>G, K820R) are associated with male infertility. PMID: 23630330
No association between LIG4 gene polymorphisms (rs1805386 T>C, rs1805389 C>T, rs1805388 C>T and rs2232641 A>G) and breast cancer risk. PMID: 22994770
Structural basis of DNA ligase IV-Artemis interaction in nonhomologous end-joining. PMID: 23219551
DNA Ligase IV is differentially required for certain chromosome fusion events induced by telomere dysfunction. PMID: 23275564
The flexibility of the DNA ligase IV catalytic region is limited in a manner that affects the formation of the LigIV/XRCC4/XLF-Cernunnos complex. PMID: 22658747
The alpha2 helix in the Lig4 BRCT-1 domain is required for adenovirus-mediated degradation of Lig4. PMID: 22534089
polymorphisms in LIG4 do not contribute to cancer risk in a population of Lynch syndrome patients with colorectal cancer PMID: 21974800
XRCC4 modulates the dynamic interaction of the Ligase IV/XRCC4 complex with the NHEJ machinery at double-stranded DNA breaks PMID: 21982441
The current results indicated that NHEJ genetic polymorphisms, particularly LIG4 rs1805388, may modulate the risk of RP in patients with NSCLC who receive definitive radio(chemo)therapy PMID: 21717429
these results suggest that some variants in XRCC4, LIG4 and Ku80 genes can contribute to thyroid cancer susceptibility PMID: 20811692
in the Chinese population, LIG4 Ile658Val has only a slight impact on the risk of developing cervical carcinoma PMID: 20400235
Polymorphisms in the LIG4, BTBD2, HMGA2, and RTEL1 genes, which are involved in the double-strand break repair pathway, are associated with glioblastoma multiforme survival. PMID: 20368557
role of NHEJ enzyme Ligase IV in the pathogenesis of MDS PMID: 19727724
provide a first sight of the structural organization of the Lig4-Xrcc4 complex, which suggests that the BRCT domains could provide the link of the ligase to Xrcc4 while permitting some movements of the catalytic domains of Lig4. PMID: 19837014
crystal structure of an Xrcc4-DNA ligase IV complex PMID: 11702069
mutations identified in patients exhibiting developmental delay and immunodeficiency PMID: 11779494
Association of DNA polymerase mu (pol mu) with Ku and ligase IV: role for pol mu in end-joining double-strand break repair. PMID: 12077346
Genetic variants of NHEJ DNA ligase IV can affect the risk of developing multiple myeloma, a tumour characterised by aberrant class switch recombination PMID: 12471202

Hide All

Involvement in disease

LIG4 syndrome (LIG4S); Severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-negative/NK-cell-positive with sensitivity to ionizing radiation (RSSCID)

Subcellular Location

Nucleus.

Protein Families

ATP-dependent DNA ligase family

Tissue Specificity

Testis, thymus, prostate and heart.

Database Links

HGNC: 6601

OMIM: 601837

KEGG: hsa:3981

STRING: 9606.ENSP00000349393

UniGene: Hs.166091

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Code	CSB-RA163614A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: Hela whole cell lysate(30µg), MCF-7 whole cell lysate(30µg), A431 whole cell lysate(30µg), THP-1 whole cell lysate(30µg), U-251MG whole cell lysate(30µg), SH-SY5Y whole cell lysate(30µg), Jurkat whole cell lysate(30µg) All lanes: DNA ligase 4 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 104 kDa Observed band size: 104 kDa Exposure time：1min IHC image of CSB-RA163614A0HU diluted at 1:100 and staining in paraffin-embedded human thyroid tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Overlay Peak curve showing jurkat cells stained with CSB-RA163614A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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LIG4 Recombinant Monoclonal Antibody

Product Details

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Target Background