LSP1 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA955655A0HU
Size	US$210
	Order now
Image	Western Blot , Positive WB detected in:Jurkat whole cell lysate(30µg), HL-60 whole cell lysate(30µg), U937 whole cell lysate(30µg) All lanes: LSP1 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 37 kDa Observed band size:52 kDa Exposure time：180s IHC image of CSB-RA955655A0HU diluted at 1:100 and staining in paraffin-embedded human gastric cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Have Questions?	Leave a Message or Start an on-line Chat

Product Details
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Uniprot No.

P33241

Target Names

LSP1

Alternative Names

47 kDa actin binding protein antibody; 47 kDa actin-binding protein antibody; 52 kDa phosphoprotein antibody; F actin binding and cytoskeleton associated protein antibody; Leufactin (leukocyte F-actin binding protein) antibody; Leufactin antibody; Leukocyte F actin binding protein antibody; Leukocyte specific protein 1 antibody; LSP 1 antibody; Lsp1 antibody; LSP1_HUMAN antibody; Lymphocyte specific antigen WP34 antibody; Lymphocyte specific protein 1 antibody; Lymphocyte specific protein pp52 antibody; Lymphocyte-specific antigen WP34 antibody; Lymphocyte-specific protein 1 antibody; OTTHUMP00000014138 antibody; OTTHUMP00000014139 antibody; OTTHUMP00000014140 antibody; OTTHUMP00000069612 antibody; OTTHUMP00000069614 antibody; OTTHUMP00000069615 antibody; OTTHUMP00000069616 antibody; pp52 antibody; Protein pp52 antibody; S37 protein antibody; WP 34 antibody; WP34 antibody

Species Reactivity

Human

Immunogen

A synthesized peptide from human LSP1 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

24F9

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

LSP1, or lymphocyte-specific protein 1, is a 37 kDa F-actin binding protein that plays a central role in regulating cytoskeletal dynamics within immune cells. Expressed predominantly in leukocytes, LSP1 influences cell motility, adhesion, and signal transduction pathways critical to immune responses. Its involvement in neutrophil and lymphocyte function makes it a valuable target for researchers investigating inflammatory processes, immune cell trafficking, and hematological malignancies.

This recombinant monoclonal antibody, clone 24F9, offers the consistency and reproducibility that demanding immunology research requires. Generated using recombinant technology with a defined sequence, this antibody eliminates the lot-to-lot variability inherent in traditional hybridoma production, ensuring your experimental results remain comparable across studies and over time. The rabbit IgG format provides excellent sensitivity while the affinity-chromatography purification yields a reagent optimized for specific target detection.

Validation studies demonstrate robust performance across multiple applications. In western blot analysis, this antibody successfully detects LSP1 in human immune cell lines including Jurkat, HL-60, and U937 whole cell lysates at dilutions ranging from 1:500 to 1:5000. The observed band at 52 kDa, rather than the predicted 37 kDa molecular weight, reflects the protein's known post-translational modifications including phosphorylation, which is consistent with LSP1's alternative designation as a 52 kDa phosphoprotein. For tissue-based studies, immunohistochemistry validation in paraffin-embedded human gastric cancer tissue confirms reliable staining at dilutions of 1:50 to 1:200.

Whether you are characterizing leukocyte populations, investigating cytoskeletal regulation, or exploring LSP1's role in disease contexts, this antibody provides a dependable tool for your immunology research.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

■ Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function

May play a role in mediating neutrophil activation and chemotaxis.

Gene References into Functions

LSP1 rs3817198 T > C polymorphism is associated with increased risk of breast cancer, especially in Caucasian and Asian populations [meta-analysis] PMID: 27590509
These results show that LSP1 is critical regulator of actomyosin contractility in primary macrophages. PMID: 29410425
Underexpression of LSP1 is associated with Breast Cancer Recurrence. PMID: 27165221
LSP1 rs569550 and rs592373 polymorphisms are both risk factors for breast cancer. PMID: 26191300
the importance of LSP1 Copy number variations and LSP1 insufficiency in the pathogenesis of rheumatoid arthritis, is reported. PMID: 26554018
The breast cancer SNP LSP1 rs3817198 was associated with an increased risk of lung cancer (odds ratio: 1.10) This association was strongest for women with adenocarcinoma (P = 1.2x10(-4)). PMID: 24681604
Data indicate that slit2N alters the localization and binding of Robo1 to WASp and LSP1 in HIV-1-gp120-treated immature dendritic cells (iDCs). PMID: 23119100
Single nucleotide polymorphism in LSP1 is associated with breast cancer. PMID: 22454379
The LSP1 rs3817198T>C polymorphism is a low-penetrant risk factor for developing breast cancer but may not be in Africans. PMID: 21127985
Low-risk variants of LSP1 is associated with familial breast cancer. PMID: 19856316
The LSP1 and 2q35 SNPs appear to interact multiplicatively on breast cancer risk for BRCA2 mutation carriers. PMID: 19656774
LSP1 interacts with F-actin and the cytoskeleton through residues downstream of amino acid residue 230. The cytoskeleton binding site of mouse LSP1 maps to the 300-330 interval. PMID: 12972289
Leukocyte-specific protein 1 is a cytoskeletal targeting protein for the ERK/MAP kinase pathway PMID: 15090600
LSP1 protein facilitates virus transport into the proteasome after its interaction with DC-SIGN through its interaction with cytoskeletal proteins. PMID: 17296787
MK2-regulated LSP1 phosphorylation is involved in stabilization of F-actin polarization during neutrophil chemotaxis. PMID: 17481585
DC-SIGN was constitutively associated with a signalosome complex consisting of the scaffold proteins LSP1, KSR1 and CNK and the kinase Raf-1 PMID: 19718030

Hide All

Subcellular Location

Cell membrane; Peripheral membrane protein; Cytoplasmic side.

Tissue Specificity

Activated T-lymphocytes.

Database Links

HGNC: 6707

OMIM: 153432

KEGG: hsa:4046

STRING: 9606.ENSP00000308383

UniGene: Hs.56729

Promotions

Email: [email protected]
Distributors Worldwide

Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

Join us with

Subscribe newsletter

Leave a message

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：
Please enter the characters shown. Click the image to refresh if unclear.

Place an order now

I. Product details

Product name *

Code *

Size *

Quantity *

Message

II. Contact details

Name *

Email address *

Company/Organization

Phone

III. Ship To

Address *

City *

State/Province *

Zip/Post code *

Country *

Attn Name *

Phone# *

IV. Bill To Same as Shipping Address