MAP2K2&MAP2K1 Recombinant Monoclonal Antibody

MAP2K1 and MAP2K2, commonly known as MEK1 and MEK2, are dual-specificity kinases that occupy a central position in the RAS-RAF-MEK-ERK signaling cascade. These kinases serve as critical signal transducers, phosphorylating and activating ERK1/2 to regulate fundamental cellular processes including proliferation, differentiation, and survival. Given their pivotal role in oncogenic signaling, MEK1/2 have become important therapeutic targets, and reliable detection tools are essential for researchers investigating MAPK pathway dynamics.

This recombinant rabbit monoclonal antibody, clone 8F10, offers the reproducibility and consistency that demanding experimental workflows require. Because the antibody sequence is defined and production occurs through recombinant expression, researchers can expect uniform performance across lots, eliminating the variability often encountered with traditional hybridoma-derived antibodies. The affinity-purified IgG format ensures high specificity for your target of interest.

Validation studies demonstrate robust performance across multiple applications. In western blot analysis, the antibody detects MEK1/2 in both human cell lines, including HeLa, Jurkat, HepG2, and SW620, as well as mouse samples such as NIH/3T3 cells and brain tissue lysate, confirming cross-species reactivity. The observed band at approximately 50 kDa runs slightly higher than the predicted molecular weights of 43-44 kDa, a shift commonly attributed to post-translational modifications such as phosphorylation. Immunofluorescence staining in A431 cells reveals clear cytoplasmic localization, while flow cytometry analysis in HeLa cells demonstrates reliable detection in single-cell applications.

This antibody serves researchers studying MAPK signaling in cancer biology, developmental processes, and therapeutic response monitoring, providing a dependable tool for interrogating this essential signaling node.