MAPRE3 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA073454A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: K562 whole cell lysate(30µg), SH-SY5Y whole cell lysate(30µg), U-251MG whole cell lysate(30µg) All lanes: MAPRE3 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 32 kDa Observed band size: 32 kDa Exposure time：2min Immunofluorescence staining of U-251MG cell with CSB-RA073454A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing SH-SY5Y cells stained with CSB-RA073454A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Target Background

Product Details

Uniprot No.

Q9UPY8

Target Names

MAPRE3

Alternative Names

Microtubule-associated protein RP/EB family member 3 (EB1 protein family member 3) (EBF3) (End-binding protein 3) (EB3) (RP3), MAPRE3

Species Reactivity

Human

Immunogen

Recombinant Antibody

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

8H1

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

MAPRE3, also known as End-binding protein 3 (EB3), plays a critical role in microtubule dynamics by tracking the plus ends of growing microtubules. As a member of the RP/EB protein family, MAPRE3 regulates microtubule stability and is involved in essential cellular processes including mitosis, cell migration, and neuronal development. Its dynamic localization patterns make it a valuable marker for studying cytoskeletal organization and intracellular transport mechanisms.

This recombinant monoclonal antibody, generated against a synthetic peptide derived from human MAPRE3, offers the consistency and reliability that demanding experimental workflows require. As a sequence-defined reagent produced through recombinant technology, it eliminates the lot-to-lot variability inherent in traditional hybridoma-derived antibodies, ensuring reproducible results across long-term studies and multi-site collaborations.

Validation studies demonstrate robust performance across multiple detection platforms. Western blot analysis confirms specific detection of MAPRE3 at the expected 32 kDa molecular weight in K562, SH-SY5Y neuroblastoma, and U-251MG glioblastoma cell lysates, providing confidence when working with diverse human cell models. Immunofluorescence staining in U-251MG cells reveals clear cytoplasmic localization patterns consistent with MAPRE3's known association with microtubule structures. Flow cytometry validation in SH-SY5Y cells further demonstrates the antibody's utility for quantitative single-cell analysis of intracellular targets.

This versatility across immunofluorescence and flow cytometry applications at working dilutions of 1:50-1:200 makes this antibody particularly well-suited for researchers investigating microtubule dynamics, cytoskeletal regulation, and neuronal cell biology. Its validated performance in neuroblastoma and glioblastoma cell lines positions it as a valuable tool for cancer research focused on cytoskeletal alterations.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Plus-end tracking protein (+TIP) that binds to the plus-end of microtubules and regulates the dynamics of the microtubule cytoskeleton. Promotes microtubule growth. May be involved in spindle function by stabilizing microtubules and anchoring them at centrosomes. Also acts as a regulator of minus-end microtubule organization: interacts with the complex formed by AKAP9 and PDE4DIP, leading to recruit CAMSAP2 to the Golgi apparatus, thereby tethering non-centrosomal minus-end microtubules to the Golgi, an important step for polarized cell movement. Promotes elongation of CAMSAP2-decorated microtubule stretches on the minus-end of microtubules. May play a role in cell migration.

Gene References into Functions

One candidate pathway coupling actin filaments to microtubules consists of the actin filament-binding protein drebrin and the microtubule-binding +TIP protein EB3. This pathway is regulated proximally by cyclin-dependent kinase 5 phosphorylation of drebrin but the upstream elements in the pathway have yet to be identified. PMID: 28865014
Findings show that co-ordination of dynamic microtubules and actin filaments by the drebrin/EB3 pathway drives prostate cancer cell invasion and is therefore implicated in disease progression. PMID: 28319065
Findings suggest that methylation-associated down-regulation of EBF3 and IRX1 genes may play an important role in a pathogenic effect of TGF-beta on RASFs. PMID: 23456299
Daughter cell adhesion and cytokinesis completion are spatially regulated by distinct states of EB3 phosphorylation on serine 176 by Aurora B. PMID: 23712260
decreasing the drebrin E levels disrupted the normal subapical F-actin-myosin-IIB-betaII-spectrin network and the apical accumulation of EB3, a microtubule-plus-end-binding protein PMID: 22275434
VE-cadherin outside-in signaling regulates cytosolic calcium homeostasis and EB3 phosphorylation. PMID: 23159740
study found a polycytosine repeat (C8) in exon 5 of MAPRE3 that could be a potential mutation target in cancers with microsatellite instability (MSI); found that the C8 is frequently mutated in gastric and colorectal carcinomas with MSI PMID: 20632835
The RP3 protein is involved in SATB1-mediated gene repression of Bcl2 and dynein subunit rp3 has functions independent of the dynein motor. PMID: 16079286
Data show that two mitotic kinases, Aurora-A and Aurora-B, phosphorylate endogenous EB3 at Ser-176, and the phosphorylation triggers disruption of the EB3-SIAH-1 complex, resulting in EB3 stabilization during mitosis. PMID: 19696028

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Subcellular Location

Cytoplasm, cytoskeleton.

Protein Families

MAPRE family

Tissue Specificity

Predominantly expressed in brain and muscle.

Database Links

HGNC: 6892

OMIM: 605788

KEGG: hsa:22924

STRING: 9606.ENSP00000233121

UniGene: Hs.515860

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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