Product Details

Uniprot No.

Target Names

Alternative Names

Methionine aminopeptidase 2 (MAP 2) (MetAP 2) (EC 3.4.11.18) (Initiation factor 2-associated 67 kDa glycoprotein) (p67) (p67eIF2) (Peptidase M), METAP2, MNPEP P67EIF2

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human Methionine Aminopeptidase 2

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

8F2

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IHC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Methionine aminopeptidase 2 (METAP2) plays a critical role in protein maturation by catalyzing the removal of N-terminal methionine residues from nascent polypeptides, a modification essential for proper protein function and stability. Beyond its enzymatic activity, METAP2 has garnered significant research interest for its involvement in angiogenesis and cell proliferation, making it an attractive target in cancer biology and metabolic disease research. The protein also functions as a regulatory component in translation initiation through its association with eIF2, earning its alternative designation as p67eIF2.

This recombinant monoclonal antibody, clone 8F2, offers researchers the reproducibility and consistency that only sequence-defined antibody production can provide. Generated in rabbit and raised against a synthetic peptide derived from human METAP2, this antibody delivers reliable performance across experimental replicates and between laboratory groups, eliminating the lot-to-lot variability that can compromise longitudinal studies.

Validation in immunohistochemistry demonstrates clear detection in paraffin-embedded human tonsil tissue using standard citrate buffer antigen retrieval at pH 6.0, with optimal staining achieved at 1:100 dilution on an automated Leica Bond system. The recommended working range of 1:50 to 1:200 provides flexibility for optimization across different tissue types and detection systems. Additional validation for ELISA applications extends the utility of this antibody for quantitative protein analysis.

For investigators exploring metabolic regulation, translational control mechanisms, or signal transduction pathways involving METAP2, this antibody provides a dependable tool for characterizing protein expression and localization in human samples.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). The catalytic activity of human METAP2 toward Met-Val peptides is consistently two orders of magnitude higher than that of METAP1, suggesting that it is responsible for processing proteins containing N-terminal Met-Val and Met-Thr sequences in vivo.; Protects eukaryotic initiation factor EIF2S1 from translation-inhibiting phosphorylation by inhibitory kinases such as EIF2AK2/PKR and EIF2AK1/HCR. Plays a critical role in the regulation of protein synthesis.

Gene References into Functions

MetAP1 and MetAP2 have roles in driving cell selectivity for a potent anti-cancer agent in synergy, by controlling glutathione redox state PMID: 27542228
Suggest MetAP2 as important regulator of proliferation/apoptosis in non-small cell lung cancers. PMID: 26935506
Nt-acetylation prevents the excision of the initiator methionine by MetAP2. PMID: 25886145
Data indicate that methionine aminopeptidase 2 (MetAP2) contains a single disulfide bond that exists in oxidized and reduced states and influences enzyme function. PMID: 24700462
The activity of unmodified, nitrated and oxidised METAP2 was assessed and it was found that nitration significantly reduced its ability to cleave a chromogenic substrate. Mass spectrometry analysis identified Tyr336 as a nitrated residue in METAP2. PMID: 24041691
Increased expression of METAP2 is associated with pilocytic astrocytoma. PMID: 23161775
This study demonstrated a hitherto-undescribed role of MetAP2 in definitive hematopoiesis and a possible link to noncanonical Wnt and ERK signaling. PMID: 21937698
the substrate specificities of Escherichia coli MetAP1, human MetAP1, and human MetAP2 were systematically profiled PMID: 20521764
Data indicate that MetAP2 expression and pp60c-src phosphorylation were decreased in NC2213 treated cells. PMID: 19703310
the functional role of S100A4 in regulating endothelial cell growth and tumor metastasis involves interaction with the N-terminal half of Methionine Aminopeptidase 2. PMID: 11994292
High expression in germinal center B cells and their neoplastic counterparts. PMID: 12118091
Divalent metal cofactors are physiologically relevant for activity of this enzyme. PMID: 12718546
MetAP protein has a role in colorectal adenocarcinoma progression PMID: 15102683
Expressed in colon cancer cells PMID: 15336565
MetAP-1 and MetAP-2 have essential functions in the control of mammalian cell proliferation PMID: 15962312
A comparison of the structual differences between Type I and Type II methionine aminopeptidases. PMID: 16274222
Data suggest that methionine aminopeptidase 2 plays a role in the proliferation of fibroblasts and myofibroblasts in fibrotic lung diseases and may serve as a novel pharmacologic target in idiopathic pulmonary fibrosis. PMID: 17446530
MetAP2 plays an important role in tumor cell growth and may contribute to tumorigenesis PMID: 18264137
Discovery, identification, and characterization of candidate pharmacodynamic markers of METAP2 inhibition are reported. PMID: 18828628
Using bovine cell extract, fumagillin was found to be bound by methionine aminopeptidase (MetAP-2). PMID: 9177176

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Subcellular Location

Cytoplasm. Note=About 30% of expressed METAP2 associates with polysomes.

Protein Families

Peptidase M24A family, Methionine aminopeptidase eukaryotic type 2 subfamily

Database Links

HGNC: 16672

OMIM: 601870

KEGG: hsa:10988

STRING: 9606.ENSP00000325312

UniGene: Hs.444986

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Code	CSB-RA294332A0HU
Size	US$210
	Order now
Image	IHC image of CSB-RA294332A0HU diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
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METAP2 Recombinant Monoclonal Antibody

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Target Background