METAP2 Antibody

Code CSB-PA013716LA01HU
Size US$166
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Image
  • Western Blot
    Positive WB detected in: Rat liver tissue
    All lanes: METAP2 antibody at 3μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 53, 52, 51 kDa
    Observed band size: 53 kDa

  • IHC image of CSB-PA013716LA01HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunofluorescence staining of HepG2 cells with CSB-PA013716LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) METAP2 Polyclonal antibody
Uniprot No.
Target Names
METAP2
Alternative Names
A930035J23Rik antibody; AI047573 antibody; AL024412 antibody; Amp2 antibody; AU014659; antibody; EIF 2 associated p67 homolog antibody; EIF-2-associated p67 antibody; Initiation factor 2 associated 67 kDa glycoprotein antibody; Initiation factor 2-associated 67 kDa glycoprotein antibody; MAP 2 antibody; MAP2 antibody; MAP2_HUMAN antibody; MetAP 2 antibody; Metap2 antibody; Methionine aminopeptidase 2 antibody; Methionyl aminopeptidase 2 antibody; MGC102452 antibody; MGC127390 antibody; MGC53792 antibody; MNPEP antibody; p67 antibody; p67eIF2 antibody; Peptidase M antibody; Peptidase M2 antibody
Raised in
Rabbit
Species Reactivity
Human, Rat
Immunogen
Recombinant Human Methionine aminopeptidase 2 protein (313-449AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The METAP2 Antibody (Product code: CSB-PA013716LA01HU) is Non-conjugated. For METAP2 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA013716LB01HU METAP2 Antibody, HRP conjugated ELISA
FITC CSB-PA013716LC01HU METAP2 Antibody, FITC conjugated
Biotin CSB-PA013716LD01HU METAP2 Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB, IHC, IF
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:20-1:200
IF 1:50-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). The catalytic activity of human METAP2 toward Met-Val peptides is consistently two orders of magnitude higher than that of METAP1, suggesting that it is responsible for processing proteins containing N-terminal Met-Val and Met-Thr sequences in vivo.; Protects eukaryotic initiation factor EIF2S1 from translation-inhibiting phosphorylation by inhibitory kinases such as EIF2AK2/PKR and EIF2AK1/HCR. Plays a critical role in the regulation of protein synthesis.
Gene References into Functions
  1. MetAP1 and MetAP2 have roles in driving cell selectivity for a potent anti-cancer agent in synergy, by controlling glutathione redox state PMID: 27542228
  2. Suggest MetAP2 as important regulator of proliferation/apoptosis in non-small cell lung cancers. PMID: 26935506
  3. Nt-acetylation prevents the excision of the initiator methionine by MetAP2. PMID: 25886145
  4. Data indicate that methionine aminopeptidase 2 (MetAP2) contains a single disulfide bond that exists in oxidized and reduced states and influences enzyme function. PMID: 24700462
  5. The activity of unmodified, nitrated and oxidised METAP2 was assessed and it was found that nitration significantly reduced its ability to cleave a chromogenic substrate. Mass spectrometry analysis identified Tyr336 as a nitrated residue in METAP2. PMID: 24041691
  6. Increased expression of METAP2 is associated with pilocytic astrocytoma. PMID: 23161775
  7. This study demonstrated a hitherto-undescribed role of MetAP2 in definitive hematopoiesis and a possible link to noncanonical Wnt and ERK signaling. PMID: 21937698
  8. the substrate specificities of Escherichia coli MetAP1, human MetAP1, and human MetAP2 were systematically profiled PMID: 20521764
  9. Data indicate that MetAP2 expression and pp60c-src phosphorylation were decreased in NC2213 treated cells. PMID: 19703310
  10. the functional role of S100A4 in regulating endothelial cell growth and tumor metastasis involves interaction with the N-terminal half of Methionine Aminopeptidase 2. PMID: 11994292
  11. High expression in germinal center B cells and their neoplastic counterparts. PMID: 12118091
  12. Divalent metal cofactors are physiologically relevant for activity of this enzyme. PMID: 12718546
  13. MetAP protein has a role in colorectal adenocarcinoma progression PMID: 15102683
  14. Expressed in colon cancer cells PMID: 15336565
  15. MetAP-1 and MetAP-2 have essential functions in the control of mammalian cell proliferation PMID: 15962312
  16. A comparison of the structual differences between Type I and Type II methionine aminopeptidases. PMID: 16274222
  17. Data suggest that methionine aminopeptidase 2 plays a role in the proliferation of fibroblasts and myofibroblasts in fibrotic lung diseases and may serve as a novel pharmacologic target in idiopathic pulmonary fibrosis. PMID: 17446530
  18. MetAP2 plays an important role in tumor cell growth and may contribute to tumorigenesis PMID: 18264137
  19. Discovery, identification, and characterization of candidate pharmacodynamic markers of METAP2 inhibition are reported. PMID: 18828628
  20. Using bovine cell extract, fumagillin was found to be bound by methionine aminopeptidase (MetAP-2). PMID: 9177176

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Subcellular Location
Cytoplasm. Note=About 30% of expressed METAP2 associates with polysomes.
Protein Families
Peptidase M24A family, Methionine aminopeptidase eukaryotic type 2 subfamily
Database Links

HGNC: 16672

OMIM: 601870

KEGG: hsa:10988

STRING: 9606.ENSP00000325312

UniGene: Hs.444986

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