Code | CSB-RA966553A0HU |
Size | US$210 |
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Application | Recommended Dilution |
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WB | 1:500-1:5000 |
IHC | 1:50-1:200 |
MGMT is a DNA repair enzyme that repairs damaged guanine nucleotides by transferring the methyl at the O6 site of guanine to its cysteine residues, thus preventing alkylating agents from causing gene mutation, cell death, and tumorigenesis. MGMT plays an important role in the resistance of tumor cells to alkylating agents, which are used in cancer treatment such as O6-alkylating agent-based chemotherapy due to their effective cytotoxic effects. Methylation of MGMT promoter enhances sensitivity to alkylating agents because DNA methylation can suppress transcription.
The main steps in the production of this MGMT recombinant antibody include immunization; harvest of positive spleen cells; obtaining the antibody sequence by screening and sequencing; expression of the target antibody in mammalian cells; purification. The MGMT antibody was produced recombinantly and has many advantages: high reproducibility, specificity and scalability. And has been validated in ELISA, WB, IHC.There are currently no reviews for this product.