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MMP11 Recombinant Monoclonal Antibody

Datasheet
Code CSB-RA110554A0HU
Size US$210
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Image
  • Western Blot
    , Positive WB detected in:HepG2 whole cell lysate(30µg), COLO205 whole cell lysate(30µg), A549 whole cell lysate(30µg), K562 whole cell lysate(30µg), THP-1 whole cell lysate(30µg), HEK293 whole cell lysate(30µg), SH-SY5Y whole cell lysate(30µg)
    All lanes: MMP11 antibody at 1:1000
    Secondary
    Goat polyclonal to rabbit IgG at 1/40000 dilution
    Predicted band size: 55 kDa
    Observed band size: 55 kDa
    Exposure time：120s
  • IHC image of CSB-RA110554A0HU diluted at 1:100 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
  • Overlay Peak curve showing HepG2 cells stained with CSB-RA110554A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Product Details

Uniprot No.
P24347
Target Names
MMP11
Alternative Names
Matrix Metalloproteinase 11 antibody; Matrix metalloproteinase-11 antibody; MMP-11 antibody; Mmp11 antibody; MMP11_HUMAN antibody; SL 3 antibody; SL-3 antibody; SL3 antibody; ST3 antibody; STMY3 antibody; Stromelysin 3 antibody; Stromelysin III antibody; Stromelysin-3 antibody
Species Reactivity
Human
Immunogen
A synthesized peptide from human MMP11 protein
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone No.
7A10
Purification Method
Affinity-chromatography
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Form
Liquid
Tested Applications
ELISA, WB, IHC, FC
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:50-1:200
FC 1:50-1:200
Protocols
ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol
Troubleshooting and FAQs
Antibody FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

Matrix metalloproteinase 11, also known as stromelysin-3, plays a distinctive role among the MMP family members in extracellular matrix remodeling and tissue homeostasis. Unlike most MMPs that are secreted as inactive zymogens, MMP11 undergoes intracellular activation, positioning it as a key player in processes involving stromal-epithelial interactions. Its expression patterns have made it particularly relevant to cardiovascular research and studies examining tissue remodeling dynamics.

This recombinant monoclonal antibody, generated from clone 7A10 in rabbit host, offers the reproducibility and consistency that demanding experimental workflows require. Because recombinant antibodies are produced from defined sequences rather than traditional hybridoma methods, you can expect reliable performance across experiments and between lot numbers, eliminating a common source of variability in long-term studies.

Validation testing demonstrates robust performance across multiple platforms. Western blot analysis confirms specific detection of MMP11 at the expected 55 kDa molecular weight across a diverse panel of human cell lines, including HepG2, COLO205, A549, K562, THP-1, HEK293, and SH-SY5Y lysates. This broad detection profile provides confidence when working with various cellular models. Immunohistochemistry validation in paraffin-embedded human prostate cancer tissue shows clear staining with standard citrate buffer antigen retrieval protocols. Flow cytometry analysis in HepG2 cells further demonstrates the antibody's utility for single-cell applications, showing distinct signal separation from isotype controls.

The antibody's compatibility with ELISA, western blotting, immunohistochemistry, and flow cytometry gives researchers flexibility to investigate MMP11 biology across complementary experimental approaches, supporting comprehensive characterization of this metalloproteinase in cardiovascular and tissue remodeling contexts.

Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Customer Reviews and Q&A

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Target Background

Function
May play an important role in the progression of epithelial malignancies.
Gene References into Functions
  1. We believe that genetic variations in the MMP-11 gene may help to predict early-stage Hepatocellular carcinoma and act as reliable biomarkers for Hepatocellular carcinoma progression PMID: 29725257
  2. In this study, matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) has been used for the first time to investigate the distribution of MMP-11 in human breast cancer tissues in order to show a possible correlation between cancerous and healthy samples, by differential proteomics and using such differences for possible cancer diagnosis and/or prognosis. PMID: 28801789
  3. Authors evaluated the expression of known targets of miR-125a and found that sirtuin-7, matrix metalloproteinase-11, and c-Raf were up-regulated in tumor tissue by 2.2-, 3-, and 1.7-fold, respectively. Overall, these data support a tumor suppressor role for miR-125a. PMID: 28445974
  4. Results revealed that MMP-11 high expression in oral squamous cell carcinoma (OSCC) samples can predict the progression, especially lymph node metastasis, and the survival of OSCC patients. PMID: 28427180
  5. A new prognostic model for HR-/HER2+ breast cancer based on the expression of MMP11 and CD2 was developed and the distant metastasis-free survival for patients in the high-risk group according to our model was significantly lower than that for those in the low-risk group. PMID: 28409241
  6. HMGA1 and MMP-11 may play a key role in the proliferation and progression of skin tumors in humans. PMID: 29374701
  7. These results demonstrated that miR-145 has an inhibitory role in TNBC malignancy by targeting MMP11 and Rab27a, which might be potential therapeutic and diagnostic targets for TNBC. PMID: 27364572
  8. the data demonstrate that miR125a5p functions as a tumor suppressor gene and serves an important role in inhibiting osteosarcoma cell migration, invasion and EMT by targeting MMP11. PMID: 27081863
  9. MMP-11 overexpression is associated with aggressive tumor phenotype and unfavorable clinical outcome in urothelial carcinomas. PMID: 26861489
  10. Our study established that high serum levels of MMP-11 are associated with poor clinical outcome and may serve as a prognostic biomarker in colon cancer patients. PMID: 27002762
  11. Overexpression of MMP-11 was discovered in sera of cancer patients compared with normal control group as well as in multiple tumor tissue specimens, such as gastric cancer, breast cancer, and pancreatic cancer. At present, some evidence supports that MMP-11 may work as a significant tumor biomarker for early detection of cancer, tumor staging, prognostic analysis, monitoring recurrence [review] PMID: 26892540
  12. Matrix metalloproteinase 11 has a role in degenerating intervertebral disc disease PMID: 26956825
  13. combined effects of the MMP-11 gene polymorphisms and environmental carcinogens are related to an increased risk for the development of OSCC and may be a predictive factor for tumor lymph node metastasis in Taiwanese with OSCC PMID: 24838924
  14. results indicated that both CD147 and MMP-11 may be involved in the progression of colorectal cancer, and they are potential prognostic factors and might become new therapeutic targets for CRC patients PMID: 26507719
  15. Our findings identified these 2 genes as a novel breast cancer biomarker gene set, which may facilitate the diagnosis and treatment in breast cancer clinical therapies. PMID: 26084486
  16. High Expression of Matrix Metalloproteinase-11 is associated with Cholangiocarcinoma. PMID: 25987024
  17. study showed that plasma level of MMP-11 may be useful for assessment of the disease progression, especially lymph node metastasis, in patients with OSCC PMID: 25423087
  18. MMP-14 function might represent one critical regulatory mechanism to control the extent of pericellular MMP-11 bioavailability and protect cells from excessive/inappropriate MMP-11 function. PMID: 25081520
  19. These findings further suggest MMP11 as a promising biomarker for breast and prostate cabcer. PMID: 24564996
  20. MMP-11 expression was significantly related to clinicopathological parameters, which may be essential to the prediction of disease outcome in patients with invasive ductal carcinoma of the breast. PMID: 23115007
  21. Knockdown of MMP11 expression could inhibit the proliferation and invasion of gastric adenocarcinoma cells probably through down-regulation of the IGF-1 signaling pathway. PMID: 23755751
  22. Findings define a regulatory role of miR-98 in tumor angiogenesis and invasion through repressed ALK4 and MMP11 expression. PMID: 23211491
  23. Data suggest that intra- and extracellular tracking of MMP-11-overexpressing cancer cells might contribute to the understanding of the activation pathways leading to MMP-11-mediated invasive processes. PMID: 22927434
  24. MiR-125a inhibits the proliferation and metastasis of hepatocellular carcinoma by targeting MMP11 and VEGF-A. PMID: 22768249
  25. expression by fibroblasts associated with poor prognosis in colorectal cancer PMID: 22488635
  26. Results describe MMP-11 expression in human epithelial colon adenocarcinoma cell lines (Caco-2, HT-29 and BCS-TC2). PMID: 22227581
  27. Report MMP11/12 expression in cervical scrapes cells from cervical precursor lesions. PMID: 22076168
  28. MMP11 (metalloproteinase 11) gene shows increased expression in papillary thyroid cancer. PMID: 17091452
  29. MMP-11 is overexpressed in many lobular carcinoma cells, and it may play role in lobular carcinogenesis through increasing resistance to anoikis. PMID: 21773755
  30. Serum levels in Chinese patients with advanced gastric carcinoma are not associated with the response to chemotherapy, but play role in lymph node metastasis and prognosis PMID: 21513571
  31. The MMP-11 is up-regulated by Gli1 and mediates the migration and invasion induced by Gli1 in MDA-MB-231. PMID: 21442356
  32. The expression of stromelysin-3 was closely associated with the invasion and metastasis of laryngeal carcinoma. PMID: 18476627
  33. matrix metalloproteinase 11 is a novel factor in the development and progression of gastric cancer and suggest that matrix metalloproteinase 11 is a marker for advanced gastric cancer. PMID: 20060156
  34. the results of this study indicate that MMP11 polymorphism may be associated with Kawasaki disease in the Korean population PMID: 20230842
  35. High expression of MMP-11 or -13, or cluster thereof, were significantly associated with higher probability of biochemical recurrence in prostate cancer PMID: 20160732
  36. MMP-11 and CK-20 are probable prognostic markers whose expression reflects the stages of tumor differentiation and LNM of breast cancer. PMID: 19914229
  37. may play role in meningioma invasiveness PMID: 11857311
  38. alternative splicing and promoter usage generates an intracellular stromelysin 3 isoform directly translated as an active matrix metalloproteinase PMID: 12006591
  39. Data confirmed stromelysin-3's contribution to breast cancer progression and its expression was shown to have a direct negative effect on patients' survival. PMID: 12429794
  40. MMP11 may have a role in the pathogenesis of papillary thyroid carcinoma PMID: 12538453
  41. Active stromelysin-3 increases breast cancer cell survival in three-dimensional Matrigel culture via activation of p42/p44 MAP-kinase PMID: 12845673
  42. ST3 protein was more involved in the pathway of colorectal cancer development in females, distal locations, infiltrative growth patterns and microsatellite stability. PMID: 15459498
  43. central role of PKC isoforms and the negative regulatory function of c-Src in the control of ST3 expression PMID: 15509588
  44. colony formation of MMP11 deficient cells was dramatically inhibited in soft agar and tumorigenicity was reduced in nude mice, respectively PMID: 15582574
  45. cathepsin F, matrix metalloproteinases 11 and 12 are upregulated in cervical cancer PMID: 15989693
  46. MMP-11, like an inflammatory mediator, may exert a chemotactic influence on macrophages which aggregate in the mesangium; MMP-11 is not likely to have a parallel mitogenic or antifibrotic effect in diseased glomeruli PMID: 17085465
  47. proposes that ST-3 induction in tumour fibroblasts leads to the stimulation of the IGF-1R pathway in carcinoma cells, thus enhancing their proliferative capacity PMID: 17233884
  48. MMP11 may play an important role in carcinogenesis and has potential implication as a biomarker for the diagnosis and prognosis of human cancers including gastric cancer. PMID: 18172255
  49. MMP11 exhibits collagenolytic function against collagen VI under malignant conditions. PMID: 18622425
  50. MMP-11 were identified at higher levels in lung secretions of pediatric ALI patients compared with controls. PMID: 19159011

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Subcellular Location
Secreted, extracellular space, extracellular matrix.
Protein Families
Peptidase M10A family
Tissue Specificity
Specifically expressed in stromal cells of breast carcinomas.
Database Links

HGNC: 7157

OMIM: 185261

KEGG: hsa:4320

STRING: 9606.ENSP00000215743

UniGene: Hs.143751

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