Product Details

Uniprot No.

Target Names

Alternative Names

Histone H3.1 (Histone H3/a) (Histone H3/b) (Histone H3/c) (Histone H3/d) (Histone H3/f) (Histone H3/h) (Histone H3/i) (Histone H3/j) (Histone H3/k) (Histone H3/l), HIST1H3A, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J, H3FA, H3FL, H3FC, H3FB, H3FD, H3FI, H3FH, H3FK, H3FF, H3FJ

Species Reactivity

Human

Immunogen

A synthesized peptide derived from Human HIST1H3A

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

13E1

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Histone H3 lysine 9 monomethylation represents a critical epigenetic mark that plays nuanced roles in chromatin organization and transcriptional regulation. Unlike the more extensively studied di- and trimethylated states at this position, H3K9me1 is associated with both active transcription and the establishment of heterochromatin, making it an important target for researchers investigating the dynamic interplay between histone modifications and gene expression programs.

This recombinant monoclonal antibody, clone 13E1, offers the reproducibility and consistency that epigenetics research demands. Produced using recombinant technology with a defined sequence, it eliminates the lot-to-lot variability that can compromise longitudinal studies or multi-site collaborations examining histone methylation patterns. The rabbit IgG format, raised against a synthetic peptide derived from human HIST1H3A, provides reliable detection of this modification in human samples.

Validation across multiple experimental platforms demonstrates genuine workflow flexibility. Immunohistochemistry studies in paraffin-embedded human gastric cancer tissue reveal clear nuclear staining patterns when using citrate buffer antigen retrieval, with effective detection at dilutions from 1:50 to 1:200. Immunofluorescence analysis in A549 human lung carcinoma cells confirms the expected nuclear localization of this histone modification, while flow cytometry validation in the same cell line shows distinct positive population shifts compared to isotype controls, enabling quantitative assessment of H3K9me1 levels at the single-cell level.

For researchers exploring epigenetic mechanisms in cancer biology, chromatin dynamics, or nuclear signaling pathways, this antibody provides a dependable tool for interrogating monomethylation status at this functionally significant lysine residue across immunohistochemical, immunofluorescent, and flow cytometric applications.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Code	CSB-RA792403A0HU
Size	US$210
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Image	IHC image of CSB-RA792403A0HU diluted at 1:100 and staining in paraffin-embedded human gastric cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.06% DAB. Immunofluorescence staining of A549 with CSB-RA792403A0HU at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 489-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing A549 cells stained with CSB-RA792403A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1µg/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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