Code | CSB-RA792403A0HU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
IHC | 1:50-1:200 |
IF | 1:50-1:200 |
FC | 1:50-1:200 |
The process for generating the mono-methyl-HIST1H3A (K9) recombinant monoclonal antibody typically begins with the incorporation of the HIST1H3A antibody-encoding gene into expression vectors. These vectors are then transferred into host cells via polyethyleneimine-mediated transfection methods. The host cells containing these vectors are cultured to produce and excrete the antibodies. After purification through affinity chromatography, the antibodies undergo evaluations involving ELISA, IHC, IF, and FC assays, demonstrating their specific binding to the human HIST1H3A protein mono-methylated at K9.
HIST1H3A mono-methylated at K9 is typically found in regions of the genome associated with heterochromatin and plays a central role in epigenetic regulation by contributing to gene repression and the formation of transcriptionally silent heterochromatin. This modification is crucial for maintaining proper gene expression patterns and cellular identity and has implications for various biological processes and disease states.
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