Code | CSB-RA943659A0HU |
Size | US$210 |
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Application | Recommended Dilution |
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IHC | 1:50-1:200 |
The production of the mutated-HIST1H3A (K27) recombinant monoclonal antibody generally begins with the incorporation of the HIST1H3A antibody-encoding gene into expression vectors. These vectors are then introduced into host cells using polyethyleneimine-mediated transfection. The host cells, housing the expression vectors, are cultured to manufacture and release the antibodies. Subsequent purification via affinity chromatography is followed by assessments through ELISA and IHC assays, verifying their ability to specifically bind to the human HIST1H3A protein mutated at K27.
HIST1H3A mutated at K27 is known as H3K27M mutation and disrupts normal epigenetic regulation, leading to altered gene expression patterns that contribute to the development of aggressive pediatric brain tumors, most notably diffuse intrinsic pontine gliomas (DIPG) and midline gliomas.
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