NAPA Recombinant Monoclonal Antibody

NAPA, also known as alpha-soluble NSF attachment protein (SNAP-alpha), plays an essential role in intracellular membrane trafficking by facilitating the disassembly of SNARE complexes after vesicle fusion events. This process is fundamental to maintaining cellular secretory pathways, neurotransmitter release, and proper protein sorting throughout the endomembrane system. Researchers investigating vesicular transport mechanisms, synaptic function, or broader signal transduction pathways will find NAPA to be a valuable marker for understanding these dynamic cellular processes.

This recombinant monoclonal antibody, clone 7F4, offers the reproducibility and consistency that demanding experimental workflows require. Because recombinant antibodies are produced from defined genetic sequences rather than traditional hybridoma methods, researchers can expect reliable performance across experiments and between reagent lots, eliminating a common source of variability in long-term studies.

The antibody has been validated for immunohistochemistry applications, with successful staining demonstrated in paraffin-embedded human colorectal cancer tissue at dilutions ranging from 1:50 to 1:200. The validation protocol employed citrate buffer antigen retrieval at pH 6.0 under high pressure conditions, followed by overnight primary antibody incubation at 4°C and detection using an HRP-conjugated goat anti-rabbit polymer system with DAB visualization. This established IHC protocol provides researchers with a reliable starting point for their own tissue-based investigations. The antibody is also suitable for ELISA applications, offering flexibility for researchers who need to incorporate multiple detection strategies into their experimental design.

For laboratories exploring signal transduction pathways, membrane trafficking dynamics, or the role of SNARE-mediated fusion in disease contexts, this antibody provides a dependable tool for detecting human NAPA in fixed tissue preparations.