Product Details

Uniprot No.

Target Names

Alternative Names

2700054G14Rik antibody; AU022060 antibody; B16.6 antibody; CDA016 antibody; Cell death regulatory protein antibody; Cell death regulatory protein GRIM-19 antibody; CGI-39 antibody; CGI39 protein antibody; CI-B16.6 antibody; Complex I-B16.6 antibody; FLJ58045 antibody; FLJ59191 antibody; Gene associated with retinoic and IFN-induced mortality 19 protein antibody; Gene associated with retinoic and interferon-induced mortality 19 protein antibody; Gene associated with retinoic interferon induced mortality 19 protein antibody; GRIM-19 antibody; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 13 antibody; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 antibody; NADH ubiquinone oxidoreductase B16.6 subunit antibody; NADH-ubiquinone oxidoreductase B16.6 subunit antibody; NDUAD_HUMAN antibody; NDUFA13 antibody; RGD1565358 antibody

Species Reactivity

Human

Immunogen

A synthesized peptide from human NDUFA13 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

4B1

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

NDUFA13, also known as GRIM-19 (Gene associated with Retinoic and Interferon-induced Mortality 19), serves a dual role in cellular biology as both a structural component of mitochondrial Complex I and a regulator of cell death pathways. This protein is essential for proper electron transport chain function and has garnered significant research interest for its involvement in apoptotic signaling and its documented interactions with STAT3, making it relevant to studies of mitochondrial dysfunction, cancer biology, and metabolic disorders.

This recombinant rabbit monoclonal antibody (clone 4B1) offers the reproducibility and consistency that demanding experimental workflows require. Generated against a synthetic peptide derived from human NDUFA13, the antibody undergoes affinity chromatography purification to ensure high specificity. Because recombinant production yields a sequence-defined reagent, researchers can expect uniform performance across experiments and between lot numbers, eliminating a common source of variability in long-term studies.

Validation data demonstrates reliable performance across multiple applications. Western blot analysis detects a clean band at the predicted 17 kDa molecular weight across diverse human cell lines including HeLa, HepG2, HEK293, MCF7, U251, and THP-1, confirming consistent detection regardless of cellular origin. Immunohistochemistry staining has been successfully performed on paraffin-embedded human kidney tissue and prostate cancer specimens, while flow cytometry validation in Jurkat cells shows clear positive signal separation from isotype controls.

This antibody supports researchers investigating mitochondrial biology, cellular metabolism, and apoptotic regulation, providing a dependable tool for characterizing NDUFA13 expression across experimental platforms from protein quantification to tissue localization and single-cell analysis.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Involved in the interferon/all-trans-retinoic acid (IFN/RA) induced cell death. This apoptotic activity is inhibited by interaction with viral IRF1. Prevents the transactivation of STAT3 target genes. May play a role in CARD15-mediated innate mucosal responses and serve to regulate intestinal epithelial cell responses to microbes.

Gene References into Functions

These results indicated that rAd-Grim-19 may regulate tumor cell growth and apoptosis. Additionally, the results demonstrated that rAdGrim19 led to beneficial outcomes and prolonged the survival of esophageal tumorbearing mice. In conclusion, the present study demonstrated that rAdGrim19 may have potential as an antitumor agent for esophageal neoplasms PMID: 29488605
Low GRIM19 expression is associated with radiation resistance in osteosarcoma. PMID: 30005830
GRIM-19 is a potential predictor of prognosis and disease recurrence in high grade serous carcinoma PMID: 29254797
Promoting the expression of GRIM19 may yield therapeutic benefits in the treatment of RA. PMID: 29306209
MiR-6743-5p may act as an oncomiRNA in glioma by targetting GRIM-19 and STAT3. PMID: 29074558
GRIM-19 suppresses the proliferation and invasion of cutaneous squamous cell carcinoma cells associated with downregulation of STAT3 signaling. PMID: 28926927
Mitochondrial GRIM-19 could not only serve as an valuable prognostic biomarker for gastric cancer development, but also as a potential therapeutic target for STAT3-dependent carcinogenesis of Gastric cancer. PMID: 27167343
GRIM-19 expression in cervical cancer cells could inhibit telomerase activity by inhibiting the transactivation of the hTERT promoter by E6, thereby promoting cervical cancer cell senescence. PMID: 27142689
NDUFA13 deficiency may be associated with asthenozoospermia through the disturbance of spermatozoa mitochondrial membrane potential and by increasing apoptosis and intracellular reactive oxygen species PMID: 27789183
GRIM-19 overexpression suppressed hepatocellular carcinoma (HCC) growth and downregulated AKT1 expression, suggesting that GRIM-19 might play a crucial role in hepatocarcinogenesis through negatively regulating the PI3K/AKT signaling pathway. PMID: 25550785
Low GRIM-19 expression is associated with paclitaxel resistance in cervical cancer. PMID: 26810068
activation of AMPKalpha by metformin was associated with a reversal of the suppressed GRIM-19 expression in H9C2 cells, the fold of changes in GRIM-19 expression by metformin were much less in HeLa cells PMID: 27101310
Data suggest that tumor expression of Ki67 (antigen Ki-67) and GRIM19 correlate with malignancy in thyroid Hurthle cell (HC) tumors; variable expression of Ki67 and GRIM19 may helped differentiate HC carcinoma from HC adenoma. PMID: 26188382
Transfection with eukaryotic plasmid for the simultaneous expression of GRIM19 and LKB1 more effectively suppressed the growth of breast cancer in vitro and in vivo, and may therefore have therapeutic potential for the treatment of human breast cancer. PMID: 26458553
Aberrant endometrial expression of GRIM-19 was associated with adenomyosis through the regulation of apoptosis and angiogenesis. PMID: 26769301
We established here a correlation between the first mutation identified in the NDUFA13 gene, which induces Mitochondrial complex I instability and a severe but slowly evolving clinical presentation affecting the central nervous system. PMID: 25901006
Upregulation of GRIM-19 also suppressed the secretion of urokinase-type plasminogen activator (u-PA), matrix metalloproteinase (MMP)-2, MMP-9 and vascular endothelial growth factor (VEGF). PMID: 25955394
GRIM-19 expression is closely associated with colorectal cancer progression and might be a very promising prognostic biomarker PMID: 26363526
Upregulation of GRIM-19 in oral squamous cell carcinoma cells significantly inhibited cell proliferation, migration and invasion in vitro and suppressed tumor growth in vivo. PMID: 25174621
decreased GRIM-19 expression due to promoter hypermethylation may be important in head and neck carcinogenesis by promoting cell proliferation and regulating metabolic activity PMID: 25575809
Simultaneous expression of ADAM10-specific siRNA and GRIM19 in HepG2 cancer cells significantly inhibited the proliferation, migration and invasion. PMID: 25242535
The GRIM-19 deficiency in the villus may be associated with missed abortion via increasing apoptosis and affecting angiogenesis. PMID: 25455534
GRIM-19 may regulate the differentiation of normal cervical tissue, and a decrease in GRIM-19 may be the result of HR-HPV infection, which in turn leads to the malignant transformation of the cells. PMID: 24690422
GRIM-19 mutations are associated with oral squamous cell carcinoma. PMID: 23851499
role in macrophage apoptosis induced by H5N1 virus PMID: 23529854
silencing of survivin and over-expression of GRIM-19 can significantly inhibit the growth and induce the apoptosis of Hep-2 laryngeal cancer cells in vitro and in vivo. PMID: 24133585
Downregulation of GRIM-19 promotes HIF1alpha synthesis. PMID: 23580587
Expressions of GRIM-19, NDUFS3, and extracellular matrix elements are correlated with invasive capabilities of breast cancer cell lines. PMID: 23630608
Down-regulation of GRIM-19 is associated with STAT3 overexpression in breast carcinomas. PMID: 23618357
GRIM-19 expression is closely correlated with histological grading and p-STAT3 in hepatocellular carcinoma PMID: 22492280
Our study found that GRIM-19 expression in lung cancer exhibits a relationship with the histological type and clinical stage PMID: 22573109
Tumor-derived mutations in the gene associated with retinoid interferon-induced mortality (GRIM-19) disrupt its anti-signal transducer and activator of transcription 3 (STAT3) activity and promote oncogenesis PMID: 23386605
import of the transcription factor STAT3 into mitochondria depends on GRIM-19, a component of the electron transport chain PMID: 23271731
GRIM-19 inhibits the STAT3 signaling pathway and sensitizes gastric cancer cells to radiation. PMID: 23124042
GRIM-19 plays a critical role not only at the origin but also in the invasive progression of hepatocellular carcinoma. PMID: 22105514
GRIM-19 expression was lower in gliomas & negatively correlated with malignancy. Downregulation enhanced cell proliferation & migration, but overexpression did the opposite. GRIM-19 exerts its role through the non-STAT3 signaling pathway. PMID: 21827581
GRIM-19 function in cancer development PMID: 21664299
The expressions of survivin and GRIM-19 may be closely correlated with the pathogenesis of prostate cancer. PMID: 21351527
These data suggest that GRIM-19 can block E6/E6AP complex; and synergistically suppress cervical tumor growth with p53. PMID: 21765936
reduced mRNA and protein levels in lung adenocarcinoma tissues PMID: 21040996
GRIM-19 expression is down-regulated in non-small cell lung cancer. PMID: 19622307
Results identify a major role for the N terminus of GRIM-19 in mediating its tumor-suppressive actions. PMID: 20595633
Alternatively spliced GRIM-19 mRNA is associated with kidney cancer. PMID: 20505682
GRIM-19/CDKN2A synergistically suppressed cell cycle progression via inhibiting E2F1-driven gene expression. PMID: 20522552
Loss of GRIM-19 is associated with invasion and metastasis of human gastric cancer. PMID: 20478305
Data show that restoration of GRIM-19 levels reestablishes the control over STAT3-dependent gene expression and tumor growth in vivo. PMID: 19642906
results collectively indicate that viral interferon regulatory factor 1 modulates interferon/retinoic acid-cell death signals via interactions with GRIM19 PMID: 12163600
GRIM-19 is an inhibitor of signal transducer and activator of transcription 3 PMID: 12867595
GRIM-19 may be a key component in NOD2-mediated innate mucosal responses and serve to regulate intestinal epithelial cell responses to microbes PMID: 15753091
The IFN-beta- and tretinoin-induced GRIM-19 is upregulated during focal cerebral ischemia. PMID: 17523870

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Involvement in disease

Hurthle cell thyroid carcinoma (HCTC)

Subcellular Location

Mitochondrion inner membrane; Single-pass membrane protein; Matrix side. Nucleus.

Protein Families

Complex I NDUFA13 subunit family

Tissue Specificity

Widely expressed, with highest expression in heart, skeletal muscle, liver, kidney and placenta. In intestinal mucosa, down-regulated in areas involved in Crohn disease and ulcerative colitis.

Database Links

HGNC: 17194

OMIM: 607464

KEGG: hsa:51079

STRING: 9606.ENSP00000423673

UniGene: Hs.534453

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Code	CSB-RA104843A0HU
Size	US$210
	Order now
Image	Western Blot , Positive WB detected in: Hela whole cell lysate(30µg), HepG2 whole cell lysate(30µg), HEK293 whole cell lysate(30µg), MCF7 whole cell lysate(30µg), U251 whole cell lysate(30µg), THP-1 whole cell lysate(30µg) All lanes: NDUFA13 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 17 kDa Observed band size: 17 kDa Exposure time：120s IHC image of CSB-RA104843A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA104843A0HU diluted at 1:100 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Overlay Peak curve showing jurkat cells stained with CSB-RA104843A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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NDUFA13 Recombinant Monoclonal Antibody

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Target Background