Product Details

Uniprot No.

P20393

Target Names

NR1D1

Alternative Names

Nuclear receptor subfamily 1 group D member 1 (Rev-erbA-alpha) (V-erbA-related protein 1) (EAR-1), NR1D1, EAR1 HREV THRAL

Species Reactivity

Human

Immunogen

A synthesized peptide from human NR1D1 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

12E8

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

NR1D1, also known as Rev-erbA-alpha, functions as a critical transcriptional repressor within the circadian clock machinery, regulating the rhythmic expression of core clock genes and metabolic pathways. This nuclear receptor plays essential roles in lipid and glucose metabolism, inflammatory responses, and adipogenesis, making it a compelling target for researchers investigating metabolic disorders, circadian biology, and cancer.

This recombinant monoclonal antibody, generated against a synthetic peptide from human NR1D1, offers the consistency and reproducibility that demanding experimental workflows require. As a sequence-defined reagent produced through recombinant technology, it eliminates the lot-to-lot variability inherent in traditional hybridoma-derived antibodies, ensuring your results remain comparable across extended studies.

Validation across multiple applications demonstrates this antibody's versatility in your research toolkit. Western blot analysis confirms specific detection in several human cell lines, including HepG2 hepatocytes, LO2 liver cells, U-251MG glioblastoma, and A431 epidermoid carcinoma cells. The observed band at 58 kDa runs slightly below the predicted 67 kDa molecular weight, a difference commonly attributed to proteolytic processing or post-translational modifications affecting electrophoretic mobility. Immunohistochemistry validation in paraffin-embedded human ovarian cancer and testis tissues demonstrates reliable performance in tissue-based studies, while flow cytometry analysis using BxPC-3 pancreatic cancer cells confirms utility for single-cell protein expression profiling.

Whether you are investigating circadian rhythm disruption in metabolic disease, exploring NR1D1 as a therapeutic target in oncology, or characterizing its expression patterns across tissue types, this antibody provides the reliable detection and experimental flexibility your research demands.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Transcriptional repressor which coordinates circadian rhythm and metabolic pathways in a heme-dependent manner. Integral component of the complex transcription machinery that governs circadian rhythmicity and forms a critical negative limb of the circadian clock by directly repressing the expression of core clock components ARTNL/BMAL1, CLOCK and CRY1. Also regulates genes involved in metabolic functions, including lipid and bile acid metabolism, adipogenesis, gluconeogenesis and the macrophage inflammatory response. Acts as a receptor for heme which stimulates its interaction with the NCOR1/HDAC3 corepressor complex, enhancing transcriptional repression. Recognizes two classes of DNA response elements within the promoter of its target genes and can bind to DNA as either monomers or homodimers, depending on the nature of the response element. Binds as a monomer to a response element composed of the consensus half-site motif 5'-[A/G]GGTCA-3' preceded by an A/T-rich 5' sequence (RevRE), or as a homodimer to a direct repeat of the core motif spaced by two nucleotides (RevDR-2). Acts as a potent competitive repressor of ROR alpha (RORA) function and regulates the levels of its ligand heme by repressing the expression of PPARGC1A, a potent inducer of heme synthesis. Regulates lipid metabolism by repressing the expression of APOC3 and by influencing the activity of sterol response element binding proteins (SREBPs); represses INSIG2 which interferes with the proteolytic activation of SREBPs which in turn govern the rhythmic expression of enzymes with key functions in sterol and fatty acid synthesis. Regulates gluconeogenesis via repression of G6PC1 and PEPCK and adipocyte differentiation via repression of PPARG. Regulates glucagon release in pancreatic alpha-cells via the AMPK-NAMPT-SIRT1 pathway and the proliferation, glucose-induced insulin secretion and expression of key lipogenic genes in pancreatic-beta cells. Positively regulates bile acid synthesis by increasing hepatic expression of CYP7A1 via repression of NR0B2 and NFIL3 which are negative regulators of CYP7A1. Modulates skeletal muscle oxidative capacity by regulating mitochondrial biogenesis and autophagy; controls mitochondrial biogenesis and respiration by interfering with the STK11-PRKAA1/2-SIRT1-PPARGC1A signaling pathway. Represses the expression of SERPINE1/PAI1, an important modulator of cardiovascular disease and the expression of inflammatory cytokines and chemokines in macrophages. Represses gene expression at a distance in macrophages by inhibiting the transcription of enhancer-derived RNAs (eRNAs). Plays a role in the circadian regulation of body temperature and negatively regulates thermogenic transcriptional programs in brown adipose tissue (BAT); imposes a circadian oscillation in BAT activity, increasing body temperature when awake and depressing thermogenesis during sleep. In concert with NR2E3, regulates transcriptional networks critical for photoreceptor development and function. In addition to its activity as a repressor, can also act as a transcriptional activator. In the ovarian granulosa cells acts as a transcriptional activator of STAR which plays a role in steroid biosynthesis. In collaboration with SP1, activates GJA1 transcription in a heme-independent manner. Represses the transcription of CYP2B10, CYP4A10 and CYP4A14. Represses the transcription of CES2. Represses and regulates the circadian expression of TSHB in a NCOR1-dependent manner. Negatively regulates the protein stability of NR3C1 and influences the time-dependent subcellular distribution of NR3C1, thereby affecting its transcriptional regulatory activity. Plays a critical role in the circadian control of neutrophilic inflammation in the lung; under resting, non-stress conditions, acts as a rhythmic repressor to limit inflammatory activity whereas in the presence of inflammatory triggers undergoes ubiquitin-mediated degradation thereby relieving inhibition of the inflammatory response. Plays a key role in the circadian regulation of microglial activation and neuroinflammation; suppresses microglial activation through the NF-kappaB pathway in the central nervous system. Plays a role in the regulation of the diurnal rhythms of lipid and protein metabolism in the skeletal muscle via transcriptional repression of genes controlling lipid and amino acid metabolism in the muscle.

Gene References into Functions

Data suggest that MYC induction of REV-ERBalpha is both persistent and recurrent across many inducible MYC model systems. PMID: 28332504
NR1D1 interacted with poly(ADP-ribose) polymerase 1 (PARP1) and subsequently inhibited catalytic activity of PARP1. PMID: 28599788
NR1D1 and BMAL1 mRNA and protein levels were significantly reduced in OA compared to normal cartilage. In cultured human chondrocytes, a clear circadian rhythmicity was observed for NR1D1 and BMAL1. PMID: 27884645
To determine the impact of REV-ERBalpha activation in the cigarette smoke (CS)-induced lung inflammatory response, we treated primary small airway epithelial cells with CS extract or lipopolysaccharide in the absence or presence of pre-treatment with the REV-ERBalpha agonist GSK 4112. PMID: 28974420
Downregulation of NR1D1 in MCF7 cells resulted in resistance to doxorubicin, both in vitro and in vivo Analysis of four public patient data sets indicated that NR1D1 expression correlates positively with clinical outcome in breast cancer patients who received chemotherapy. Our findings suggest that NR1D1 and its ligands provide therapeutic options that could enhance the outcomes of chemotherapy in breast cancer patients PMID: 28249904
Highly quantitative fluorescence anisotropy assays in competition mode revealed that the rev-erbA-alpha specificity for the NCoR corepressor lies in the first two residues of the beta-strand in Interaction Domain 1 of NCoR. PMID: 25969949
Data show that ubiquitin E3 ligase Siah2 depletion delays circadian degradation of nuclear hormone receptor RevErbalpha (Nr1d1) and lengthens period length. PMID: 26392558
the role of NR1D1 polymorphisms in the regulation of Nuclear receptor REV-ERBalpha and circadian rhythms regulation PMID: 25798852
associations between NR1D1, RORA and RORB genes and bipolar disorder.( PMID: 25789810
CRY2 and REV-ERB ALPHA as the clock genes upregulated in obesity during the 24 h period and that REV-ERB ALPHA is an important gene associated with MS. PMID: 25365257
Our results suggest that the REV-ERB ALPHA rs939347 polymorphism could modulate body fat mass in men. The present work supports the role of REV-ERB ALPHA in the development of obesity as well as a potential target for the treatment of obesity PMID: 25089907
Using free-energy simulations, the study shows that rev-erbA-alpha-induced DNA deformation preferentially occurs by induced fit rather than by conformational selection, even though the DNA is only slightly distorted in the complex. PMID: 24677802
Rev-erbalpha is a novel regulator of hepatic stellate cell transdifferentation PMID: 24497272
apoA-IV inhibits hepatic gluconeogenesis by decreasing Glc-6-Pase and PEPCK gene expression through NR1D1. PMID: 24311788
Rev-erbalpha bestows protection against mycobacterial infection by direct gene repression of IL10 and thus provide a novel target in modulating macrophage microbicidal properties. PMID: 23449984
DBC1 modulates the stability and function of the nuclear receptor Rev-erb-alpha. PMID: 23398316
A haplotype block in REV-ERBalpha was associated with white matter lesion volumes in the Rotterdam Study I. PMID: 23083441
Genetic variants of NR1D1 associate with bipolar disorder. PMID: 22538398
Thyroid hormone receptor-alpha/NR1D1 polymorphisms were not associated with baseline characteristics, including serum TSH and free thyroxine. None of the polymorphisms were associated with bone mineral density or osteoporotic fractures. PMID: 22224817
in situ hybridization revealed that RORA and NR1D1 were expressed in human endometrial stromal and epithelial cells PMID: 22024429
REV-ERBalpha plays a dual role in regulation of the activity of the BMAL1/CLOCK heterodimer by regulation of expression of both the BMAL1 and CLOCK genes. PMID: 21479263
Data demonstrate that NPAS2 is also a RORalpha and REV-ERBalpha target gene. PMID: 20817722
Study reports the crystal structure of a nuclear receptor-co-repressor (N-CoR) interaction domain 1 (ID1) peptide bound to truncated human Rev-erbalpha ligand-binding domain. PMID: 20581824
Arf-bp1 and Pam are novel regulators of circadian gene expression that target Rev-erb alpha for degradation PMID: 20534529
Results show that the cells of this aggressive form of breast cancer are genetically preprogrammed to depend on NR1D1 and PBP for the energy production necessary for survival. PMID: 20160030
Review. We propose that the nuclear receptor and core clock component Rev-erb-alpha behaves as a gatekeeper to timely coordinate the circadian metabolic response. PMID: 19696364
While negative, our findings do not exclude an involvement of DGKH and NR1D1 in lithium prophylaxis. PMID: 19818381
novel physiological role for members of the Rev-erb family of nuclear receptors in the regulation of genes involved in triglyceride metabolism and the pathogenesis of atherosclerosis PMID: 12021280
ROR(alpha) and Reverb(alpha) are expressed with a similar tissue distribution and are both induced during the differentiation of rat L6 myoblastic cells PMID: 12114512
hRev-erbalpha plays a key role in hRORalpha1 action PMID: 12377782
Data show that Rev-erb alpha and beta contain a functional nuclear location signal in the DNA-binding domain, and suggest that they control their intracellular localization via a mechanism different from that of other nuclear receptors. PMID: 12683943
Rev-Erbalpha as a target gene of PPARgamma in adipose tissue and demonstrate a role for this nuclear receptor as a promoter of adipocyte differentiation. PMID: 12821652
Overexpression of EAR1 upregulated expression of IL6 and COX2, and increased transactivation by NFKB and nuclear translocation of p65 in A7r5 VSMCs. The expression of EAR1 was upregulated by RORalpha1 but that upregulation was attenuated by EAR1 itself PMID: 15013753
findings show that GSK3beta phosphorylates and stabilizes Rev-erbalpha, a negative component of the circadian clock; control of Rev-erbalpha protein stability is a critical component of the peripheral clock and a biological target of lithium therapy PMID: 16484495
Rev-ErbAalpha is highly expressed in osteoarthritis articular chondrocytes and that its expression is modulated by known cartilage catabolic and anabolic stimuli PMID: 17075855
findings show that rev-erbalpha serves as a heme sensor that coordinates the cellular clock, glucose homeostasis, and energy metabolism PMID: 18006707
heme regulation of REV-ERBs may link the control of metabolism and the mammalian clock PMID: 18037887
Rev-erbalpha protein levels must rise and then fall for adipocyte differentiation to occur. Stable expression of Rev-erb protein prevents induction of PPARgamma2. PMID: 18227153
These data identify Rev-erbalpha as a new LXR target gene, inhibiting LXR-induction of TLR-4 in a negative transcriptional feedback loop, but not cholesterol homeostasis gene expression. PMID: 18511497
This study suggests that NR1D1 does not play a major role in the pathophysiology of mood disorders in the Japanese population. PMID: 18804497
This study indicated a nominal association of the REV-ERBalpha gene with bipolar disorder, suggest a possible role of REV-ERBalpha in the pathogenesis of bipolar disorder. PMID: 19267705
Rev-erbalpha modulates the synthesis of its own ligand in a negative feedback pathway that maintains heme levels and regulates cellular energy metabolism. PMID: 19710360

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Subcellular Location

Nucleus. Cytoplasm. Cell projection, dendrite. Cell projection, dendritic spine.

Protein Families

Nuclear hormone receptor family, NR1 subfamily

Tissue Specificity

Widely expressed. Expressed at high levels in the liver, adipose tissue, skeletal muscle and brain. Also expressed in endothelial cells (ECs), vascular smooth muscle cells (VSMCs) and macrophages. Expression oscillates diurnally in the suprachiasmatic nuc

Database Links

HGNC: 7962

OMIM: 602408

KEGG: hsa:9572

STRING: 9606.ENSP00000246672

UniGene: Hs.592130

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Code	CSB-RA957337A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: HepG2 whole cell lysate(30µg), LO2 whole cell lysate(30µg), U-251MG whole cell lysate(30µg), A431 whole cell lysate(30µg) All lanes: NR1D1 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 67 kDa Observed band size: 58 kDa Exposure time：2min IHC image of CSB-RA957337A0HU diluted at 1:100 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA957337A0HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Overlay Peak curve showing BxPC-3 cells stained with CSB-RA957337A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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NR1D1 Recombinant Monoclonal Antibody

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Target Background