Code | CSB-RA843829A0HU |
Size | US$210 |
Order now | |
Image |
|
Have Questions? | Leave a Message or Start an on-line Chat |
Application | Recommended Dilution |
---|---|
WB | 1:500-1:2000 |
IHC | 1:50-1:200 |
CUSABIO implemented a meticulous process to produce the NSDHL recombinant monoclonal antibody. Initially, an animal was immunized with a synthesized peptide derived from human NSDHL. After that, B cells were isolated from the spleen of the immunized animal. Extracted RNA was converted into cDNA through reverse transcription. The cDNA served as a template to amplify the gene encoding the NSDHL antibody. The NSDHL antibody gene was subsequently inserted into a vector. Through transfection, the recombinant vector was introduced into host cells, allowing for the efficient expression of the NSDHL recombinant monoclonal antibodies. These antibodies were harvested from the cell culture supernatant and purified using affinity chromatography. Stringent validation, including thorough ELISA, WB, and IHC testing, was conducted to verify the NSDHL recombinant monoclonal antibody's specific reactivity with human NSDHL protein, ensuring its reliability and suitability for diverse applications.
There are currently no reviews for this product.