NUDT19 Recombinant Monoclonal Antibody

NUDT19, also known as Nudix motif 19, belongs to the Nudix hydrolase family of enzymes that play essential roles in cellular metabolism by hydrolyzing nucleoside diphosphate derivatives. This mitochondrial protein participates in the regulation of cellular nucleotide pools and has garnered attention in signal transduction research, where understanding metabolic enzyme function provides insights into cellular homeostasis and disease mechanisms.

This recombinant monoclonal antibody, clone 5B2, offers researchers the consistency and reliability that comes with sequence-defined production. Unlike traditional hybridoma-derived antibodies, recombinant technology ensures lot-to-lot reproducibility, meaning your experimental conditions remain stable across long-term studies and collaborative projects. The rabbit host and monoclonal nature provide high specificity for human NUDT19, with affinity chromatography purification delivering a clean reagent ready for demanding applications.

Validation data demonstrates robust performance across multiple experimental platforms. Western blot analysis confirms detection of the expected 42 kDa band across a diverse panel of human cell lines, including A431, MCF-7, A549, HEK293, K562, SH-SY5Y, and THP-1, providing confidence when working with various cellular models. Immunohistochemistry staining has been successfully performed in paraffin-embedded human kidney tissue and colorectal cancer samples, supporting tissue-based investigations. Flow cytometry validation in HeLa cells shows clear positive signal separation from isotype controls, enabling quantitative single-cell analysis of NUDT19 expression.

Whether you are investigating mitochondrial metabolism, exploring nucleotide signaling pathways, or characterizing NUDT19 expression patterns in disease contexts, this antibody provides the experimental flexibility to move seamlessly between protein quantification, tissue localization, and cell population studies.