NXF1 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA160494A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: THP-1 whole cell lysate(30µg), Hela whole cell lysate(30µg), HEK293T whole cell lysate(30µg), K562 whole cell lysate(30µg) All lanes: NXF1 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 70 kDa Observed band size: 70 kDa Exposure time：2min IHC image of CSB-RA160494A0HU diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA160494A0HU diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of Hela cell with CSB-RA160494A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing jurkat cells stained with CSB-RA160494A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Target Background

Product Details

Uniprot No.

Q9UBU9

Target Names

NXF1

Alternative Names

Nuclear RNA export factor 1 (Tip-associated protein) (Tip-associating protein) (mRNA export factor TAP), NXF1, TAP

Species Reactivity

Human

Immunogen

A synthesized peptide from human NXF1 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

23F2

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

NXF1, also known as TAP (Tip-associated protein), serves as a central component of the mRNA nuclear export machinery, facilitating the transport of mature mRNA from the nucleus to the cytoplasm for translation. This essential function places NXF1 at a critical junction in gene expression regulation, making it a valuable target for researchers investigating mRNA processing, nuclear transport mechanisms, and related pathways in cancer biology and viral infection studies.

This recombinant monoclonal antibody (clone 23F2) offers the reproducibility and consistency that demanding experimental workflows require. Because it is generated from a defined sequence rather than traditional hybridoma methods, you can expect uniform performance across lots, eliminating the variability that can complicate long-term studies or multi-site collaborations.

Validation data demonstrates robust performance across multiple applications, providing flexibility for diverse experimental approaches. Western blot analysis confirms specific detection of NXF1 at the expected 70 kDa molecular weight across several human cell lines, including THP-1, HeLa, HEK293T, and K562, indicating reliable performance in commonly used research models. Immunohistochemistry staining has been validated in human glioma cancer tissue and small intestine sections, supporting investigations in both oncology and normal tissue contexts. For subcellular localization studies, immunofluorescence analysis in HeLa cells reveals clear staining patterns, while flow cytometry validation in Jurkat cells demonstrates suitability for quantitative single-cell analysis.

Whether you are exploring mRNA export dynamics, characterizing nuclear transport defects in disease models, or investigating NXF1's emerging roles in viral RNA trafficking, this antibody provides a dependable tool backed by comprehensive application-specific validation data.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Involved in the nuclear export of mRNA species bearing retroviral constitutive transport elements (CTE) and in the export of mRNA from the nucleus to the cytoplasm (TAP/NFX1 pathway). The NXF1-NXT1 heterodimer is involved in the export of HSP70 mRNA in conjunction with ALYREF/THOC4 and THOC5 components of the TREX complex. ALYREF/THOC4-bound mRNA is thought to be transferred to the NXF1-NXT1 heterodimer for export. Also involved in nuclear export of m6A-containing mRNAs: interaction between SRSF3 and YTHDC1 facilitates m6A-containing mRNA-binding to both SRSF3 and NXF1, promoting mRNA nuclear export.

Gene References into Functions

Gain or loss-of-function experiments revealed NXF1 selectively regulates TLR7-driven IRF5 transcriptional activity, suggesting a new role for NXF1 in the IRF5 signaling pathway. PMID: 28578407
repeat RNA-sequestration of SRSF1 triggers the NXF1-dependent nuclear export of C9ORF72 transcripts retaining expanded hexanucleotide repeats PMID: 28677678
Hepatitis B virus core protein associates with the cellular NXF1-p15 complex via the nuclear export signal motif of hepatitis B virus core protein.Nuclear export of human hepatitis B virus core protein and pregenomic RNA depends on the NXF1-p15 machinery. PMID: 25360769
Complementary structural, biochemical and cellular techniques indicated that the formation of a symmetric RNA binding platform generated by dimerization of NXF1:NXT1 facilitates the recognition of CTE-RNA and promotes its nuclear export PMID: 25628361
Gag polyprotein synthesis was decreased by NXF1 knockdown. PMID: 25212909
TREX is the major complex used to recruit Nxf1 to mRNA in human cells. PMID: 22893130
the organization of the NXF1 proline-tyrosine nuclear localization signal reveal unexpected redundancy in the nuclear import pathways used by NXF1. PMID: 21965294
The U2AF65 protein served as an adaptor to link expanded CAG RNA to NXF1 for RNA export. PMID: 21725067
The crystal structure of the RNA recognition and leucine-rich repeat motifs of TAP bound to one symmetrical half of the CTE RNA is solved. PMID: 21822283
these data revealed a redox-regulated chaperone function of PDI in delivering antigenic peptides from TAP to MHC-I. PMID: 21299467
Tpr plays an important role in quality control of mRNA trafficked on the Nxf1 pathway. PMID: 21613532
These results showed that the interaction between herpes simplex virus type 1 ICP27 and human TAP/NXF1 occurred in living cells upon head-to-tail intramolecular association of ICP27. PMID: 21060739
Varicella-zoster virus IE4 protein interacts with SR proteins and exports mRNAs through the TAP/NXF1 pathway PMID: 19924249
Depletion of NXF1 or 5,6-dichloro-1-beta-d-ribofuranosyl-benzimidazole treatment had similar effects, inhibiting the nuclear export of several of the H5N1 influenza virus mRNAs. PMID: 20071484
The structural and mutational data define a novel RNA-binding site on the Tap protein PMID: 11854490
Structure of the C-terminal FG-nucleoporin binding domain of Tap/NXF1 PMID: 11875519
Karyopherin beta2B is a nuclear export factor and mediates mRNA export in cooperation with TAP PMID: 12384575
structural basis for interaction of UBA domain and FG nucleoporins PMID: 12581645
nuclear export sequences regulate human TAP function in mRNA export by direct nuclear pore interactions PMID: 14963046
hypophosphorylation of ASF in mRNPs coincides with its higher affinity for TAP PMID: 15184380
SR proteins 9G8 and ASF/SF2 exhibit higher affinity for TAP/NXF1 when hypophosphorylated PMID: 15210956
concluded that herpes simplex virus type 1 ICP27 and the viral RNAs to which it binds are exported via the TAP/NXF1 export receptor. PMID: 15767397
results indicate that Tap regulates expression of its own intron-containing RNA through a CTE (constitutive transport element)-mediated mechanism PMID: 16971948
RNA transport element RTE evolved as a high affinity RBM15 ligand to provide a splicing-independent link to NXF1, thereby ensuring efficient nuclear export and expression of retrotransposon transcripts PMID: 17001072
evidence presented that the export factor TAP/NXF1 binds poorly to XIST RNA in comparison to exported mRNAs, suggesting that reduced TAP/NFX1 binding may contribute to nuclear retention of XIST RNA PMID: 17333237
ORF57 interacts with the cellular export factor REF and with RNA, suggesting that it may provide target mRNAs with access to REF, which mediates nuclear RNA export by binding to TAP/NXF1. PMID: 17609285
Homotypic Tap complex can interact with both Nxt1 and nucleoporins in vitro. PMID: 17978099
The efficiency and stability of the approach are demonstrated by reconstructing the structure of a two domain region of the 31 kDa nuclear export factor TAP (TIP-associated protein). PMID: 18670889
ICP27 is the major export adaptor for HSV-1 mRNA and that it links bound transcripts to the TAP/NXF1 export receptor PMID: 19019956
The RNA-binding motif protein 15B (RBM15B/OTT3) acts as cofactor of the nuclear export receptor NXF1. PMID: 19586903
oligomerization of Tap affects its interactions with nucleoporins PMID: 17978099

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Subcellular Location

Nucleus. Nucleus, nucleoplasm. Nucleus speckle. Nucleus, nuclear pore complex. Nucleus envelope. Cytoplasm. Cytoplasm, Stress granule.

Protein Families

NXF family

Tissue Specificity

Expressed ubiquitously.

Database Links

HGNC: 8071

OMIM: 602647

KEGG: hsa:10482

STRING: 9606.ENSP00000294172

UniGene: Hs.523739

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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