Product Details

Uniprot No.

Target Names

Alternative Names

Phosphoglycerate mutase 1 (EC 5.4.2.11) (EC 5.4.2.4) (BPG-dependent PGAM 1) (Phosphoglycerate mutase isozyme B) (PGAM-B), PGAM1, PGAMA

Species Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human PGAM1

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

9A5

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Phosphoglycerate mutase 1 (PGAM1) serves as a critical enzyme in glycolysis, catalyzing the interconversion of 3-phosphoglycerate and 2-phosphoglycerate. Beyond its metabolic function, PGAM1 has emerged as a significant player in cancer biology, where its upregulation supports the enhanced glycolytic activity characteristic of rapidly proliferating tumor cells. This metabolic reprogramming makes PGAM1 an attractive target for researchers investigating cancer metabolism, signal transduction pathways, and potential therapeutic interventions.

This recombinant monoclonal antibody, clone 9A5, offers the reproducibility and consistency that demanding experimental workflows require. Because it is produced from a defined sequence rather than traditional hybridoma methods, you can expect uniform performance across different lots, eliminating the variability that can complicate long-term studies or multi-site collaborations. The rabbit IgG format, raised against a synthetic peptide derived from human PGAM1, provides excellent specificity for your target.

Validation by western blot demonstrates robust detection across an impressive range of sample types, including human cell lines such as HeLa, 293, HepG2, A549, and MCF-7, as well as mouse NIH/3T3 cells and both mouse and rat brain tissue lysates. This cross-species reactivity spanning human, mouse, and rat samples provides flexibility for comparative studies or translational research models. The antibody detects a band at approximately 29 kDa, slightly above the predicted 28 kDa molecular weight, a minor shift likely attributable to post-translational modifications. Recommended working dilutions range from 1:500 to 1:5000, allowing optimization for your specific experimental conditions.

Whether you are exploring metabolic alterations in cancer models or investigating PGAM1's role in cellular signaling, this antibody delivers the reliable performance your research demands.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Interconversion of 3- and 2-phosphoglycerate with 2,3-bisphosphoglycerate as the primer of the reaction. Can also catalyze the reaction of EC 5.4.2.4 (synthase), but with a reduced activity.

Gene References into Functions

High PGAM1 expression is associated with Pancreatic Ductal Adenocarcinoma Metastasis. PMID: 29386088
conformation and dynamics of the C-terminal region in human phosphoglycerate mutase 1 PMID: 28748916
When BPGM is knocked out, 1,3-BPG can directly phosphorylate PGAM1. In this case, PGAM1 phosphorylation and activity are decreased, but nevertheless sufficient to maintain normal glycolytic flux and cellular growth rate. PMID: 28805803
study provided the first evidence revealing a non-metabolic function of PGAM1 in promoting cell migration, and gained new insights into the role of PGAM1 in cancer progression. PMID: 27991922
Data show that tyrosine 26 phosphorylation enhances the binding of phosphoglycerate mutase 1 (PGAM1) to its substrates through generating electrostatic environment and structural features that are advantageous to the binding. PMID: 28076845
PGAM1 in promoting homologous recombination repair in tumor cell lines. Suggest potential therapeutic opportunity for PGAM1 inhibitors in combination with PARP inhibitors in cancer treatment. PMID: 28122957
PGAM1 may be associated with the grade of glioma and be involved in the biological behavior of glioma cells. PGAM1 might be a novel therapeutic target in glioma. PMID: 27572934
PGAM1 correlates with spermatogenic dysfunction and affects the function of cell proliferation, apoptosis and migration. PMID: 25701843
Our finding showed that PGAM1 might serve as a promising therapeutic target for UBC. PMID: 26655504
PGAM1 is highly expressed in clear cell renal cell carcinoma and correlated with clinicalpathological features, which may contribute to tumor formation and progression. PMID: 26464696
PGAM is acetylated at lysines 100/106/113/138 in its central region, and a member of the Sirtuin family (class III deacetylase), SIRT2, is responsible for its deacetylation. PMID: 25195573
Tyrosine26 phosphorylation represents an additional acute mechanism underlying phosphoglycerate mutase 1 upregulation. PMID: 23653202
Phosphoglycerate mutase 1 (PGAM1) contributes to biosynthesis regulation by controlling intracellular levels of its substrate, 3-phosphoglycerate (3-PG), and product, 2-phosphoglycerate (2-PG). PMID: 23153533
PGAM1 deacetylation and activity are directly controlled by Sirt1. PMID: 22157007
histidine-phosphorylated PGAM1 correlated with expression of PKM2 in cancer cell lines; decreased pyruvate kinase activity in PKM2-expressing cells allows PEP-dependent histidine phosphorylation of PGAM1 and may provide an alternate glycolytic pathway PMID: 20847263
Our studies suggested that PGAM1 plays an important role in hepatocarcinogenesis PMID: 20403181
Results identified phosphoglycerate mutase 1 that showed elevated levels of protein carbonyls in inferior parietal lobule (IPL) from subjects with early stage-Alzheimer's disease. PMID: 19686046
Cloning, purification, crystallization and preliminary crystallographic analysis of human phosphoglycerate mutase PMID: 15388943
Phosphoglycerate mutase may have a role in preventing non-spherocytic anemia [case report] PMID: 15710582
We report two patients in whom phosphoglycerate mutase (PGAM) deficiency was associated with the triad of exercise-induced cramps, recurrent myoglobinuria, and tubular aggregates in the muscle biopsy. PMID: 16881065

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Protein Families

Phosphoglycerate mutase family, BPG-dependent PGAM subfamily

Tissue Specificity

Expressed in the liver and brain. Not found in the muscle.

Database Links

HGNC: 8888

OMIM: 172250

KEGG: hsa:5223

STRING: 9606.ENSP00000359991

UniGene: Hs.632918

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Code	CSB-RA935337A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate, NIH/3T3 whole cell lysate, A549 whole cell lysate, MCF-7 whole cell lysate, Mouse Brain whole cell lysate, Rat Brain whole cell lysate All lanes: PGAM1 Antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 28 kDa Observed band size: 29 kDa
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