PGAM1 Recombinant Monoclonal Antibody

Code CSB-RA935337A0HU
Size US$210
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  • Western Blot
    Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate, NIH/3T3 whole cell lysate, A549 whole cell lysate, MCF-7 whole cell lysate, Mouse Brain whole cell lysate, Rat Brain whole cell lysate
    All lanes: PGAM1 Antibody at 1:1000
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 28 kDa
    Observed band size: 29 kDa
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Product Details

Uniprot No.
Target Names
PGAM1
Alternative Names
Phosphoglycerate mutase 1 (EC 5.4.2.11) (EC 5.4.2.4) (BPG-dependent PGAM 1) (Phosphoglycerate mutase isozyme B) (PGAM-B), PGAM1, PGAMA
Species Reactivity
Human, Mouse, Rat
Immunogen
A synthesized peptide derived from human PGAM1
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone No.
9A5
Purification Method
Affinity-chromatography
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
Liquid
Tested Applications
ELISA, WB
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

To produce the PGAM1 recombinant antibody, four main steps are involved. First, the PGAM1 monoclonal antibody gene is sequenced, followed by the cloning of the gene into a plasmid vector. The recombinant vector is then introduced into a host cell line, and the PGAM1 recombinant monoclonal antibody is purified from the cell culture supernatant using affinity chromatography. Finally, the purified antibody is tested and characterized. The PGAM1 monoclonal antibody is derived from hybridomas that produce the PGAM1 antibody, with a synthesized peptide derived from human PGAM1 being used as the immunogen during generation. The resulting PGAM1 recombinant monoclonal antibody is highly recommended for ELISA and WB applications to detect PGAM1 protein from human, mouse, and rat species.

PGAM1 is an enzyme that plays a crucial role in the glycolysis pathway, which is the primary metabolic pathway that converts glucose into pyruvate to produce ATP (adenosine triphosphate) for cellular energy. Specifically, PGAM1 catalyzes the conversion of 3-phosphoglycerate (3-PG) to 2-phosphoglycerate (2-PG) in the second step of glycolysis. This reaction generates a molecule of ATP and a molecule of water, and it is reversible, allowing the enzyme to function in both the glycolysis and gluconeogenesis pathways. In addition to its role in energy metabolism, PGAM1 has also been implicated in the regulation of cell proliferation and cancer progression.

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Target Background

Function
Interconversion of 3- and 2-phosphoglycerate with 2,3-bisphosphoglycerate as the primer of the reaction. Can also catalyze the reaction of EC 5.4.2.4 (synthase), but with a reduced activity.
Gene References into Functions
  1. High PGAM1 expression is associated with Pancreatic Ductal Adenocarcinoma Metastasis. PMID: 29386088
  2. conformation and dynamics of the C-terminal region in human phosphoglycerate mutase 1 PMID: 28748916
  3. When BPGM is knocked out, 1,3-BPG can directly phosphorylate PGAM1. In this case, PGAM1 phosphorylation and activity are decreased, but nevertheless sufficient to maintain normal glycolytic flux and cellular growth rate. PMID: 28805803
  4. study provided the first evidence revealing a non-metabolic function of PGAM1 in promoting cell migration, and gained new insights into the role of PGAM1 in cancer progression. PMID: 27991922
  5. Data show that tyrosine 26 phosphorylation enhances the binding of phosphoglycerate mutase 1 (PGAM1) to its substrates through generating electrostatic environment and structural features that are advantageous to the binding. PMID: 28076845
  6. PGAM1 in promoting homologous recombination repair in tumor cell lines. Suggest potential therapeutic opportunity for PGAM1 inhibitors in combination with PARP inhibitors in cancer treatment. PMID: 28122957
  7. PGAM1 may be associated with the grade of glioma and be involved in the biological behavior of glioma cells. PGAM1 might be a novel therapeutic target in glioma. PMID: 27572934
  8. PGAM1 correlates with spermatogenic dysfunction and affects the function of cell proliferation, apoptosis and migration. PMID: 25701843
  9. Our finding showed that PGAM1 might serve as a promising therapeutic target for UBC. PMID: 26655504
  10. PGAM1 is highly expressed in clear cell renal cell carcinoma and correlated with clinicalpathological features, which may contribute to tumor formation and progression. PMID: 26464696
  11. PGAM is acetylated at lysines 100/106/113/138 in its central region, and a member of the Sirtuin family (class III deacetylase), SIRT2, is responsible for its deacetylation. PMID: 25195573
  12. Tyrosine26 phosphorylation represents an additional acute mechanism underlying phosphoglycerate mutase 1 upregulation. PMID: 23653202
  13. Phosphoglycerate mutase 1 (PGAM1) contributes to biosynthesis regulation by controlling intracellular levels of its substrate, 3-phosphoglycerate (3-PG), and product, 2-phosphoglycerate (2-PG). PMID: 23153533
  14. PGAM1 deacetylation and activity are directly controlled by Sirt1. PMID: 22157007
  15. histidine-phosphorylated PGAM1 correlated with expression of PKM2 in cancer cell lines; decreased pyruvate kinase activity in PKM2-expressing cells allows PEP-dependent histidine phosphorylation of PGAM1 and may provide an alternate glycolytic pathway PMID: 20847263
  16. Our studies suggested that PGAM1 plays an important role in hepatocarcinogenesis PMID: 20403181
  17. Results identified phosphoglycerate mutase 1 that showed elevated levels of protein carbonyls in inferior parietal lobule (IPL) from subjects with early stage-Alzheimer's disease. PMID: 19686046
  18. Cloning, purification, crystallization and preliminary crystallographic analysis of human phosphoglycerate mutase PMID: 15388943
  19. Phosphoglycerate mutase may have a role in preventing non-spherocytic anemia [case report] PMID: 15710582
  20. We report two patients in whom phosphoglycerate mutase (PGAM) deficiency was associated with the triad of exercise-induced cramps, recurrent myoglobinuria, and tubular aggregates in the muscle biopsy. PMID: 16881065

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Protein Families
Phosphoglycerate mutase family, BPG-dependent PGAM subfamily
Tissue Specificity
Expressed in the liver and brain. Not found in the muscle.
Database Links

HGNC: 8888

OMIM: 172250

KEGG: hsa:5223

STRING: 9606.ENSP00000359991

UniGene: Hs.632918

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