PPCS Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA790244A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: Hela whole cell lysate(30µg), Jurkat whole cell lysate(30µg), HEK293 whole cell lysate(30µg), HepG2 whole cell lysate(30µg), HT-29 whole cell lysate(30µg), A431 whole cell lysate(30µg), MCF-7 whole cell lysate(30µg), NIH/3T3 whole cell lysate(30µg) All lanes: PPCS antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 34 kDa Observed band size: 34 kDa Exposure time：10s IHC image of CSB-RA790244A0HU diluted at 1:100 and staining in paraffin-embedded mouse kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
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Product Details
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Target Background

Product Details

Uniprot No.

Q9HAB8

Target Names

PPCS

Alternative Names

COAB antibody; FLJ11838 antibody; MGC117357 antibody; MGC138220 antibody; OTTHUMP00000008433 antibody; Phosphopantothenate--cysteine ligase antibody; Phosphopantothenoylcysteine synthetase antibody; PPC synthetase antibody; Ppcs antibody; PPCS_HUMAN antibody; RP11-163G10.1 antibody

Species Reactivity

Human, Mouse

Immunogen

A synthesized peptide from human PPCS protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

6D12

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Catalyzes the second step in the biosynthesis of coenzyme A from vitamin B5, where cysteine is conjugated to 4'-phosphopantothenate to form 4-phosphopantothenoylcysteine. Has a preference for ATP over CTP as a cosubstrate.

Gene References into Functions

Exome sequencing in five individuals from two unrelated families presenting with dilated cardiomyopathy revealed biallelic mutations in PPCS. PMID: 29754768
Oxygen transfer studies found that 18O from [carboxyl-18O] phosphopantothenate is incorporated into AMP or CMP produced during PPCS catalysis, consistent with the formation of a phosphopantothenoyl cytidylate or phosphopantothenoyl adenylate intermediate PMID: 19683078

Protein Families

PPC synthetase family

Database Links

HGNC: 25686

OMIM: 609853

KEGG: hsa:79717

STRING: 9606.ENSP00000361642

UniGene: Hs.706662

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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