PPCS Recombinant Monoclonal Antibody

PPCS, or phosphopantothenate-cysteine ligase, catalyzes a critical step in coenzyme A biosynthesis by coupling phosphopantothenate with cysteine. This essential enzymatic function positions PPCS as a key player in cellular metabolism, influencing fatty acid synthesis, the citric acid cycle, and numerous acyl-transfer reactions that underpin fundamental biological processes. Researchers investigating metabolic regulation, signal transduction pathways, and cellular energetics will find PPCS an informative target for understanding how cells maintain their biosynthetic capacity.

This recombinant monoclonal antibody, generated in rabbit against a synthetic peptide derived from human PPCS, offers the reproducibility and consistency that demanding experimental workflows require. Because recombinant antibodies are produced from defined sequences, researchers can expect uniform performance across experiments and between lots, eliminating the variability that can complicate longitudinal studies or collaborative projects.

Validation studies demonstrate robust performance across multiple applications. In western blot analysis, the antibody detects a clean 34 kDa band matching the predicted molecular weight of PPCS, with successful detection confirmed across a diverse panel of human cell lines including HeLa, Jurkat, HEK293, HepG2, HT-29, A431, and MCF-7, as well as mouse NIH/3T3 cells, supporting cross-species reactivity between human and mouse samples. Immunohistochemistry validation in paraffin-embedded mouse kidney tissue further demonstrates the antibody's utility for examining PPCS expression in tissue contexts, with optimized protocols using citrate buffer antigen retrieval.

Whether examining PPCS expression patterns across different cell types, investigating its role in metabolic signaling networks, or exploring tissue-specific distribution, this antibody provides researchers with a reliable tool for advancing their understanding of coenzyme A metabolism and related cellular processes.