PPM1G Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA213346A0HU
Size	US$210
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Image	IHC image of CSB-RA213346A0HU diluted at 1:100 and staining in paraffin-embedded human colorectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Overlay Peak curve showing Hela cells stained with CSB-RA213346A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Target Background

Product Details

Uniprot No.

O15355

Target Names

PPM1G

Alternative Names

Protein phosphatase 1G (EC 3.1.3.16) (Protein phosphatase 1C) (Protein phosphatase 2C isoform gamma) (PP2C-gamma) (Protein phosphatase magnesium-dependent 1 gamma), PPM1G, PPM1C

Species Reactivity

Human

Immunogen

A synthesized peptide from human PPM1G protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

5E2

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IHC, FC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

PPM1G, also known as protein phosphatase 2C isoform gamma, is a serine/threonine phosphatase that plays essential roles in pre-mRNA splicing regulation and cellular stress responses. This phosphatase dephosphorylates key substrates involved in spliceosome assembly and has emerged as a significant player in understanding RNA processing mechanisms and their connections to disease states, including cancer biology.

This recombinant monoclonal antibody, clone 5E2, offers researchers the reproducibility advantages inherent to recombinant technology. Because the antibody sequence is defined and produced from a stable expression system, you can expect consistent performance across experiments and over time, eliminating the lot-to-lot variability that can complicate long-term studies or multi-site collaborations. The rabbit IgG format, purified by affinity chromatography, ensures high specificity for human PPM1G.

Validation studies demonstrate reliable performance across multiple experimental platforms. In immunohistochemistry applications, this antibody successfully detected PPM1G in paraffin-embedded human colorectal cancer tissue using a standard citrate buffer antigen retrieval protocol on an automated Leica Bond system, with recommended dilutions ranging from 1:50 to 1:200. Flow cytometry validation using HeLa cells confirmed intracellular detection capability, showing clear positive signal separation from isotype control when cells were fixed and permeabilized prior to staining. The antibody is also validated for ELISA applications, providing flexibility for quantitative studies.

This PPM1G antibody serves researchers investigating splicing regulation, phosphatase signaling networks, and cancer-related pathways where PPM1G dysregulation may contribute to disease progression. Its validated performance in both tissue sections and cell-based assays makes it suitable for studies spanning basic mechanistic research to translational investigations.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Gene References into Functions

PPM1G phosphatase directly binds 7SK RNA and the kinase inhibitor Hexim1 once P-TEFb has been released from the 7SK snRNP. PMID: 26324325
Hypermethylation in the 3'-protein-phosphatase-1G (PPM1G) gene locus was associated with alcohol use disorder. PMID: 25982659
PPM1G inactivates monomeric WWP2 and promotes the assembly of WWP2-WWP1 heterodimeric complex. PMID: 25071155
PPM1G dephosphorylates the P-TEFb kinase T loop to disassemble the 7SK snRNP. PMID: 24316072
study has identified protein phosphatase PPM1G as a novel regulator of cap-dependent protein translation by negatively controlling the phosphorylation of 4E-BP1. PMID: 23814053
ATM-dependent downregulation of USP7/HAUSP by PPM1G activates p53 response to DNA damage PMID: 22361354
The degradation of p21(WAF1/CIP1) induced by PP2Cgamma was mediated in a proteasome-dependent manner. PMID: 17054950
Protein phosphatase 2C gamma mediates histone dephosphorylation/exchange in response to DNA damage or checkpoint recovery in higher eukaryotes. PMID: 17074886
PP2Cgamma modulates alternative splicing of specific pre-mRNAs coregulated by YB-1. PMID: 17572683
an interplay between PPM1G and unrip determine compartment-specific phosphorylation patterns, localization, and function of the SMN complex PMID: 17984321
PP2Cgamma is a novel regulator of p21(Cip1/WAF1) protein stability via the Akt signaling pathway. PMID: 19538940
Functional study of the mouse homolog PMID: 9271424

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Subcellular Location

Cytoplasm. Membrane; Lipid-anchor.

Protein Families

PP2C family

Tissue Specificity

Widely expressed. Most abundant in testis, skeletal muscle, and heart.

Database Links

HGNC: 9278

OMIM: 605119

KEGG: hsa:5496

STRING: 9606.ENSP00000264714

UniGene: Hs.643951

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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