PRKCD/PRKCQ/Prkcd Recombinant Monoclonal Antibody

Protein kinase C delta and theta isoforms represent critical nodes in cellular signaling networks, mediating diverse processes including apoptosis, immune cell activation, and proliferation. PKC delta functions as a tumor suppressor in many contexts and plays essential roles in oxidative stress responses, while PKC theta is particularly important in T cell receptor signaling and immune function. The involvement of these kinases in cancer biology, inflammation, and metabolic regulation makes them valuable targets for researchers investigating fundamental cell biology and disease mechanisms.

This recombinant monoclonal antibody, produced from a stable rabbit host and defined by clone 24F7, offers the reproducibility that demanding experimental workflows require. Because recombinant production ensures sequence-defined consistency between lots, researchers can confidently compare results across extended studies without concerns about batch-to-batch variation that can complicate data interpretation with traditional hybridoma-derived antibodies.

Validation across multiple platforms demonstrates genuine experimental flexibility. Western blot analysis confirms detection at the expected 78 kDa molecular weight across diverse human cell lines including THP-1, A431, Jurkat, HT-29, MCF7, and A375, with cross-species reactivity confirmed in mouse and rat brain tissue lysates. Immunohistochemistry staining has been validated in human lung tissue and liver cancer sections using citrate buffer antigen retrieval, while immunofluorescence studies in HeLa cells reveal clear cytoplasmic localization patterns. Flow cytometry applications have been demonstrated in A431 cells with distinct signal separation from isotype controls.

The broad application compatibility and validated cross-species reactivity make this antibody particularly suitable for comparative studies examining PKC delta and theta signaling in oncology research, immunology, and neuroscience applications where consistent, reliable detection is essential.