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PRKCG/PRKCB/PRKCA/PRKCE/PRKCZ/PRKCD Recombinant Monoclonal Antibody

PRKCG/PRKCB/PRKCA/PRKCE/PRKCZ/PRKCD Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA584389A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: COLO-205 whole cell lysate(30µg), Mouse brain tissue lysate(30µg), Rat brain tissue lysate(30µg) All lanes: PKC antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 37, 40, 68-84 kDa Observed band size: 77 kDa Exposure time：30s IHC image of CSB-RA584389A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA584389A0HU diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Overlay Peak curve showing BxPC-3 cells stained with CSB-RA584389A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Product Details
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Product Details

Uniprot No.

P05129 P05771 P17252 Q02156 Q05513 Q05655

Target Names

PRKCG/PRKCB/PRKCA/PRKCE/PRKCZ/PRKCD

Alternative Names

KPCG_HUMAN antibody; MGC57564 antibody; OTTHUMP00000067291 antibody; PKC-gamma antibody; PKCC antibody; PKCG antibody; PRKCG antibody; Protein kinase C gamma antibody; Protein kinase C gamma polypeptide antibody; Protein kinase C gamma type antibody; Protein kinase C; gamma antibody; SCA 14 antibody; SCA14 antibody; KPCB_HUMAN antibody; PKC Beta antibody; PKC-B antibody; PKC-beta antibody; PKCB antibody; Prkcb antibody; PRKCB II antibody; PRKCB2 antibody; Protein kinase C beta antibody; Protein kinase C beta type antibody; KPCA_HUMAN antibody; PKC alpha antibody; PKC beta antibody; PKC delta antibody; PKC epsilon antibody; PKC gamma antibody; PKC zeta antibody; PKC-A antibody; PKC-alpha antibody; PKC2 antibody; PKCA antibody; PKCB antibody; PKCD antibody; PKCE antibody; PKCG antibody; PRKCA antibody; PRKCB antibody; PRKCB1 antibody; PRKCB2 antibody; PRKCD antibody; PRKCE antibody; PRKCG antibody; PRKCZ antibody; Protein kinase C alpha antibody; Protein kinase C alpha type antibody; Protein kinase C antibody; Protein kinase C beta antibody; Protein kinase C delta antibody; Protein kinase C epsilon antibody; Protein kinase C gamma antibody; Protein kinase C zeta antibody; KPCE_HUMAN antibody; MGC125656 antibody; MGC125657 antibody; nPKC epsilon antibody; nPKC-epsilon antibody; PKCE antibody; Pkcea antibody; PRKCE antibody; Protein kinase C epsilon antibody; Protein kinase C epsilon type antibody; 14-3-3-zetaisoform antibody; AI098070 antibody; aPKCzeta antibody; C80388 antibody; EC 2.7.11.13 antibody; KPCZ_HUMAN antibody; nPKC zeta antibody; nPKC-zeta antibody; OTTHUMP00000001368 antibody; OTTHUMP00000044160 antibody; PKC 2 antibody; PKC ZETA antibody; PKC2 antibody; Pkcz antibody; PKCZETA antibody; PKM-zeta; included antibody; PRKCZ antibody; Protein kinase C zeta antibody; Protein kinase C zeta form antibody; Protein kinase C zeta type antibody; r14-3-3 antibody; R74924 antibody; zetaPKC antibody; CVID9 antibody; D14Ertd420e antibody; Kinase PKC delta antibody; KPCD antibody; KPCD_HUMAN antibody; MAY 1 antibody; MAY1 antibody; MGC49908 antibody; nPKC delta antibody; nPKC-delta antibody; PCKd antibody; PKC d antibody; PKC delta antibody; PKCD antibody; PKCdelta antibody; PRKC D antibody; PRKC delta antibody; Prkcd antibody; Protein Kinase C delta antibody; Protein kinase C delta type antibody; Protein kinase C delta VIII antibody; Protein Kinase Cdelta antibody; Tyrosine protein kinase PRKCD antibody

Species Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide from human PRKCG/PRKCB/PRKCA/PRKCE/PRKCZ/PRKCD protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

6A2

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

The protein kinase C family represents a cornerstone of cellular signal transduction, with individual isoforms orchestrating diverse processes from cell proliferation and differentiation to apoptosis and immune responses. This recombinant monoclonal antibody targets multiple PKC isoforms including PRKCA, PRKCB, PRKCG, PRKCD, PRKCE, and PRKCZ, making it particularly valuable for researchers investigating the broader PKC signaling network or comparing isoform expression patterns across experimental conditions.

Developed using recombinant technology and produced as clone 6A2, this rabbit monoclonal antibody offers the reproducibility essential for longitudinal studies and multi-site collaborations. The sequence-defined nature of recombinant production ensures consistent performance across lots, eliminating the variability that can complicate data interpretation when tracking subtle changes in kinase expression or activation states.

Validation across multiple platforms demonstrates genuine experimental flexibility. Western blot analysis confirms detection in human COLO-205 cells as well as mouse and rat brain tissue lysates, with observed bands at approximately 77 kDa. This molecular weight aligns with the larger PKC isoforms and likely reflects post-translational modifications, particularly glycosylation, which are characteristic of these kinases in their native cellular context. The antibody's cross-species reactivity across human, mouse, and rat samples supports translational research workflows moving between model systems.

Immunohistochemistry validation in human kidney and liver cancer tissue sections expands utility into spatial expression studies, while flow cytometry performance in BxPC-3 pancreatic cancer cells enables single-cell analysis of PKC levels. This combination of validated applications positions the antibody well for cancer biology research, neuroscience investigations, and studies exploring PKC-mediated signaling in metabolic disease.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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