Product Details

Uniprot No.

Target Names

Alternative Names

Pre-mRNA-processing factor 19 (EC 2.3.2.27) (Nuclear matrix protein 200) (PRP19/PSO4 homolog) (hPso4) (RING-type E3 ubiquitin transferase PRP19) (Senescence evasion factor), PRPF19, NMP200 PRP19 SNEV

Species Reactivity

Human

Immunogen

A synthesized peptide from human PRPF19 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

11A8

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

PRPF19 serves as a critical component of the spliceosome, functioning as a RING-type E3 ubiquitin ligase essential for pre-mRNA processing and genome stability. Beyond its core role in splicing, PRPF19 has garnered significant research interest for its involvement in DNA damage response pathways and cellular senescence, where it acts as a senescence evasion factor. These diverse functions make PRPF19 a compelling target for researchers investigating RNA processing mechanisms, aging biology, and cancer progression.

This recombinant monoclonal antibody, generated in rabbit against a synthetic peptide from human PRPF19, offers the consistency and reliability that demanding experimental workflows require. As a sequence-defined recombinant clone, it eliminates the lot-to-lot variability inherent in traditional hybridoma-derived antibodies, ensuring reproducible results across extended studies and collaborative projects.

Validation across multiple detection platforms demonstrates this antibody's versatility in your research toolkit. Immunohistochemistry studies in paraffin-embedded human testis tissue reveal clear target detection using citrate buffer antigen retrieval at dilutions of 1:50–1:200. Immunofluorescence analysis in PC-3 prostate cancer cells shows distinct cellular localization patterns, while flow cytometry experiments using Jurkat cells confirm robust performance in single-cell analysis applications, with clear separation between specific staining and isotype control populations.

The antibody's compatibility with fixed and permeabilized samples across these platforms provides flexibility for researchers examining PRPF19 expression in tissue sections, cultured cells, or suspension cell populations. Whether your work focuses on spliceosome biology, DNA repair mechanisms, or the molecular underpinnings of cellular aging and transformation, this antibody delivers the specificity and reproducibility essential for meaningful experimental outcomes.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Ubiquitin-protein ligase which is a core component of several complexes mainly involved pre-mRNA splicing and DNA repair. Required for pre-mRNA splicing as component of the spliceosome. Core component of the PRP19C/Prp19 complex/NTC/Nineteen complex which is part of the spliceosome and participates in its assembly, its remodeling and is required for its activity. During assembly of the spliceosome, mediates 'Lys-63'-linked polyubiquitination of the U4 spliceosomal protein PRPF3. Ubiquitination of PRPF3 allows its recognition by the U5 component PRPF8 and stabilizes the U4/U5/U6 tri-snRNP spliceosomal complex. Recruited to RNA polymerase II C-terminal domain (CTD) and the pre-mRNA, it may also couple the transcriptional and spliceosomal machineries. The XAB2 complex, which contains PRPF19, is also involved in pre-mRNA splicing, transcription and transcription-coupled repair. Beside its role in pre-mRNA splicing PRPF19, as part of the PRP19-CDC5L complex, plays a role in the DNA damage response/DDR. It is recruited to the sites of DNA damage by the RPA complex where PRPF19 directly ubiquitinates RPA1 and RPA2. 'Lys-63'-linked polyubiquitination of the RPA complex allows the recruitment of the ATR-ATRIP complex and the activation of ATR, a master regulator of the DNA damage response. May also play a role in DNA double-strand break (DSB) repair by recruiting the repair factor SETMAR to altered DNA. As part of the PSO4 complex may also be involved in the DNA interstrand cross-links/ICLs repair process. In addition, may also mediate 'Lys-48'-linked polyubiquitination of substrates and play a role in proteasomal degradation. May play a role in the biogenesis of lipid droplets. May play a role in neural differentiation possibly through its function as part of the spliceosome.

Gene References into Functions

This work implies that the gain of Prp19 is a critical event during the progression of hepatocellular carcinoma PMID: 26959880
These results demonstrate that SNEV regulates adipogenesis in hASCs and indicate that DDR capacity in general might be a pre-requisite for this process. PMID: 28041875
the interactome of influenza A virus NS1 in host cells was comprehensively profiled, and our findings reveal a novel regulatory role for PRP19 in viral replication PMID: 27096427
Crystal structure revealed features of the WD40 domain of human PRPF19. PMID: 28962858
The results suggest that RPA phosphorylation enhances the recruitment of PRP19 to RPA-ssDNA and stimulates RPA ubiquitylation through a process requiring both PRP19 and RFWD3, thereby triggering a phosphorylation-ubiquitylation circuitry that promotes ATR activation and homologous recombination. PMID: 28666352
role of Pso4 in DNA repair and DNA damage PMID: 26364595
Study demonstrates that the levels of Prp19 and Cdc5L are overexpressed in hepatocellular carcinoma (HCC). Prp19 binds with Cdc5L and its downregulation results in reduction of Cdc5L. Mechanistic insights reveal that silencing Prp19 compromises translation activity of Cdc5L and facilitates lysosome-induced degradation of Cdc5L in HCC cells. PMID: 28387715
We report that PRP19 expression is elevated in lung carcinoma tissues compared to non-tumor tissues PMID: 24731397
These findings suggest that SKAP promotes ultraviolet rays-induced cell apoptosis by negatively regulating the anti-apoptotic protein Prp19. PMID: 24718257
involvement of hPso4 in the recombinational repair of DSBs PMID: 24675077
DNA damage induces down-regulation of Prp19 via impairing Prp19 stability in hepatocellular carcinoma cells. PMID: 24587161
The Prp19-CDC5L complex plays a crucial role during human pre-mRNA splicing by catalytic activation of the spliceosome. PMID: 24598747
Prp19 complex as the first spliceosome subcomplex that directly contributes to mitosis in vertebrates independently of its function in interphase. PMID: 24069358
PRP19 directly binds RPA and localizes to DNA damage sites via RPA, promoting RPA ubiquitylation in a DNA-damage-induced manner. PMID: 24332808
New insights into pre-mRNA processing factor 19 PMID: 22967061
PRP19 over-expression is associated with hepatocellular carcinoma recurrence. PMID: 23220010
SNEV phosphorylation at S149 is essential for mediating the cytoprotective effect of SNEV upon DNA damage/oxidative stress and partially contributes to the life span extension caused by SNEV overexpression. PMID: 22529335
hPso4, once it forms a complex with Metnase, negatively regulates Metnase's TIR binding activity PMID: 20416268
Purified hPrp19/CDC5L complexes exhibit an elongated, asymmetric shape with a maximum dimension of approximately 20 nm. PMID: 20176811
concluded that hPso4 plays a major and previously undefined role in mammalian DNA DSB repair[Pso4] PMID: 12960389
required prior to catalytic step 1 of splicing, but not for stable integration of U4/U6.U5 tri-snRNPs PMID: 15175653
report the identification of two additional factors required in the DNA interstrand cross-links repair process, a previously characterized pre-mRNA splicing complex composed of Pso4/Prp19 and Werner syndrome protein(WRN) PMID: 16223718
SNEV, the human orthologue of yeast PRP19, functions in splicing and homo-oligomerization of SNEV in HeLa nuclear extract is essential for spliceosome assembly. PMID: 16332694
The high levels of SNEV might extend the cellular life span by increasing the resistance to stress or by improving the DNA repair capacity of the cells. PMID: 16388800
ubiquitylated hPrp19 fails to interact with either Cdc5L or Plrg1 indicating that DNA damage can induce profound alterations to hPrp19 core complex. PMID: 17276391
hPso4 is necessary to bring Metnase to the DSB sites for its function(s) in DNA repair PMID: 18263876
Blom7alpha is a novel splicing factor of the K homology domain family that might be implicated in alternative splicing by helping to position the CDC5L-SNEV(Prp19-Pso4) complex at the splice sites [Blom7alpha] PMID: 19641227

Hide All

Subcellular Location

Nucleus. Nucleus, nucleoplasm. Cytoplasm, cytoskeleton, spindle. Cytoplasm. Lipid droplet.

Protein Families

WD repeat PRP19 family

Tissue Specificity

Ubiquitous. Weakly expressed in senescent cells of different tissue origins. Highly expressed in tumor cell lines.

Database Links

HGNC: 17896

OMIM: 608330

KEGG: hsa:27339

STRING: 9606.ENSP00000227524

UniGene: Hs.502705

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Code	CSB-RA904109A0HU
Size	US$210
	Order now
Image	IHC image of CSB-RA904109A0HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of PC-3 cell with CSB-RA904109A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing jurkat cells stained with CSB-RA904109A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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PRPF19 Recombinant Monoclonal Antibody

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