PUM1 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA090772A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: HEK293 whole cell lysate(30µg),COLO-205 whole cell lysate(30µg) All lanes: Pumilio 1 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 126 kDa Observed band size: 140 kDa Exposure time：2min IHC image of CSB-RA090772A0HU diluted at 1:100 and staining in paraffin-embedded human colorectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Overlay Peak curve showing Hela cells stained with CSB-RA090772A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Target Background

Product Details

Uniprot No.

Q14671

Target Names

PUM1

Alternative Names

Pumilio homolog 1 (HsPUM) (Pumilio-1), PUM1, KIAA0099 PUMH1

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human PUM1

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

9A9

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

PUM1 (Pumilio homolog 1) is an RNA-binding protein that plays a central role in post-transcriptional gene regulation, controlling mRNA stability and translation through sequence-specific interactions with target transcripts. As a key regulator of stem cell maintenance, neuronal function, and cell cycle progression, PUM1 has emerged as an important research target in developmental biology, neuroscience, and cancer studies where dysregulated RNA metabolism contributes to disease pathology.

This recombinant monoclonal antibody, generated against a synthetic peptide derived from human PUM1, offers the reproducibility and consistency that demanding experimental workflows require. Because the antibody sequence is defined and production occurs in a controlled recombinant system, researchers can expect reliable performance across experiments and between lots, eliminating the variability often encountered with traditional hybridoma-derived antibodies.

Validation studies demonstrate robust performance across multiple applications. In western blot analysis, the antibody detects PUM1 in HEK293 and COLO-205 whole cell lysates at dilutions of 1:500–1:2000, producing a band at approximately 140 kDa. The observed molecular weight exceeds the predicted 126 kDa, which is consistent with post-translational modifications such as glycosylation commonly observed with this protein. Immunohistochemistry studies confirm specific staining in paraffin-embedded human colorectal cancer tissue, while flow cytometry analysis in HeLa cells shows clear separation from isotype controls, demonstrating utility for intracellular protein detection in fixed and permeabilized samples.

This antibody serves researchers investigating RNA-protein interactions, translational control mechanisms, and PUM1's contributions to oncogenesis and neurological disorders, providing a dependable tool for characterizing this multifunctional regulatory protein across complementary experimental platforms.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Sequence-specific RNA-binding protein that acts as a post-transcriptional repressor by binding the 3'-UTR of mRNA targets. Binds to an RNA consensus sequence, the Pumilio Response Element (PRE), 5'-UGUANAUA-3', that is related to the Nanos Response Element (NRE). Mediates post-transcriptional repression of transcripts via different mechanisms: acts via direct recruitment of the CCR4-POP2-NOT deadenylase leading to translational inhibition and mRNA degradation. Also mediates deadenylation-independent repression by promoting accessibility of miRNAs. Following growth factor stimulation, phosphorylated and binds to the 3'-UTR of CDKN1B/p27 mRNA, inducing a local conformational change that exposes miRNA-binding sites, promoting association of miR-221 and miR-222, efficient suppression of CDKN1B/p27 expression, and rapid entry to the cell cycle. Acts as a post-transcriptional repressor of E2F3 mRNAs by binding to its 3'-UTR and facilitating miRNA regulation. Represses a program of genes necessary to maintain genomic stability such as key mitotic, DNA repair and DNA replication factors. Its ability to repress those target mRNAs is regulated by the lncRNA NORAD (non-coding RNA activated by DNA damage) which, due to its high abundance and multitude of PUMILIO binding sites, is able to sequester a significant fraction of PUM1 and PUM2 in the cytoplasm. Involved in neuronal functions by regulating ATXN1 mRNA levels: acts by binding to the 3'-UTR of ATXN1 transcripts, leading to their down-regulation independently of the miRNA machinery. Plays a role in cytoplasmic sensing of viral infection. In testis, acts as a post-transcriptional regulator of spermatogenesis by binding to the 3'-UTR of mRNAs coding for regulators of p53/TP53. Involved in embryonic stem cell renewal by facilitating the exit from the ground state: acts by targeting mRNAs coding for naive pluripotency transcription factors and accelerates their down-regulation at the onset of differentiation. Binds specifically to miRNA MIR199A precursor, with PUM2, regulates miRNA MIR199A expression at a postranscriptional level.

Gene References into Functions

Variants in PUM1 may not contribute to primary ovarian insufficiency in Han Chinese women PMID: 29297114
PUM1 SNP is associated with Osteoporosis and Obesity. PMID: 29145611
Results indicate that pumilio RNA binding family member 1 (PUM1) is a negative regulator of RNA helicase LGP2 (LGP2), a master regulator of innate immunity genes expressed in a cascade fashion. PMID: 28760986
Thus, PUM1 promotes the development and progression of ovarian cancer, which may occur via the above-mentioned molecules. PMID: 29428722
A recent paper by the Mendell group identifies NORAD, a novel lncRNA that is regulated in response to DNA damage and plays a key role in maintaining genome integrity by modulating the activity the RNA binding proteins PUM2 and PUM1. PMID: 27157388
results reveal a novel regulatory pathway, underscoring a previously unknown and interconnected key role of PUM1/2 and FOXP1 in regulating normal hematopoietic stem/progenitor cell and leukemic cell growth. PMID: 28232582
Pumilios (including PUM1 and PUM2) are RNA-binding proteins with Puf domains made up of 8 poorly conserved Puf repeats, 3 helix bundles arranged in rainbow architecture, where each repeat recognizes a single base of the RNA-binding sequence. [REVIEW] PMID: 26517885
Identification of NORAD-interacting proteins revealed that this lncRNA functions as a multivalent binding platform for PUM proteins, with the capacity to sequester a significant fraction of the total cellular pool of PUM1 and PUM2.Studies reveal unanticipated roles for a lncRNA and PUMILIO proteins in the maintenance of genomic stability. PMID: 26724866
PUM repeats have now been identified in proteins that function in pre-rRNA processing, including human Puf-A and yeast Puf6. PMID: 25512524
The promoting effect of hPuf-A in tumorigenesis might be correlated with the regulation of its associated mRNAs. PMID: 23625657
MED15 and PUM1 proteins with coiled-coil domains are potent enhancers of polyQ-mediated ataxin-1 protein misfolding and proteotoxicity in vitro and in vivo. PMID: 22916034
human Pumilio homologs Pum 1 and Pum 2 repress the translation of E2F3 by binding to the E2F3 3' untranslated region (UTR) and also enhance the activity of multiple E2F3 targeting microRNAs (miRNAs) PMID: 22345517
Three distinct modes of target RNA binding by PUM1 around the fifth mRNA base were observed. PMID: 21397187
Pumilio-1 (PUM1) is a ubiquitously expressed RBP that was shown to interact with p27-3' UTR. PMID: 20818387
The reference genes of choice when performing RT-qPCR on normal and malignant breast specimens should be either the collected group of 3 genes (TBP, RPLP0 and PUM1) employed as an average, or PUM1 as a single gene. PMID: 18211679
Description of the structures of hPum Puf domain complexed to two noncognate RNAs, CycB(reverse) and Puf5. PMID: 18328718
ribonomic analysis of mRNAs associated with ribonucleoproteins containing an endogenous human PUF protein, Pum1 PMID: 18411299
Puf-A transcripts were uniformly distributed in early embryos, but became restricted primarily to eyes and ovaries at a later stage of development PMID: 19319195

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Subcellular Location

Cytoplasm. Cytoplasm, P-body. Cytoplasmic granule.

Tissue Specificity

Expressed in brain, heart, kidney, muscle, intestine and stomach. Not expressed in cerebellum, corpus callosum, caudate nucleus, hippocampus, medulla oblongata and putamen. Expressed in all fetal tissues tested.

Database Links

HGNC: 14957

OMIM: 607204

KEGG: hsa:9698

STRING: 9606.ENSP00000391723

UniGene: Hs.281707

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No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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