Phospho-CDK1/CDK2/CDK3 (T14) Recombinant Monoclonal Antibody

Cyclin-dependent kinases 1, 2, and 3 serve as master regulators of cell cycle progression, with their activity tightly controlled through phosphorylation at conserved residues. Phosphorylation at threonine 14 represents a critical inhibitory modification that prevents premature kinase activation, ensuring proper timing of cell division. Detecting this specific phosphorylation event provides researchers with a direct window into cell cycle checkpoint regulation, DNA damage responses, and the mechanisms that maintain genomic stability.

This recombinant monoclonal antibody, derived from clone 4C12, offers the reproducibility essential for phospho-specific detection studies. Because the antibody sequence is defined and produced recombinantly in rabbit, researchers can expect consistent performance across experiments and between lots, eliminating the variability that often complicates longitudinal studies or multi-site collaborations. The affinity-chromatography purification further ensures high specificity for the phosphorylated epitope.

Validation in western blot applications demonstrates reliable detection in human cell models, with clear 34 kDa bands observed in HeLa whole cell lysates and A549 cells treated with either Calyculin A or EGF. These treatment conditions, which modulate phosphatase activity and growth factor signaling respectively, confirm the antibody's utility for studying dynamic phosphorylation changes in response to cellular stimuli. The observed band size matches the predicted molecular weight, indicating specific recognition of the target proteins. Recommended working dilutions of 1:500 to 1:5000 for western blotting provide flexibility to optimize signal intensity based on your experimental system.

For researchers investigating cell cycle control, checkpoint signaling, or the regulatory mechanisms governing CDK activity, this antibody delivers the specificity and consistency needed to generate reliable, publication-quality data.