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| Code | CSB-RA361848A14phHU |
| Size | US$210 |
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| Application | Recommended Dilution |
|---|---|
| WB | 1:500-1:5000 |
To manufacture the phospho-CDK1/CDK2/CDK3 (T14) recombinant monoclonal antibody, the journey begins with the retrieval of genes responsible for encoding the CDK1/CDK2/CDK antibody from rabbits that have previously been exposed to a synthesized peptide derived from the human CDK1/CDK2/CDK protein phosphorylated at T14. Subsequently, these antibody genes are skillfully cloned into specialized expression vectors. Following this genetic modification, the modified vectors are carefully introduced into host suspension cells, which are then diligently cultured to promote the expression and secretion of antibodies. After this cultivation phase, the phospho-CDK1/CDK2/CDK3 (T14) recombinant monoclonal antibody is meticulously purified from the cell culture supernatant through the application of affinity chromatography. Finally, the antibody's functionality is rigorously assessed through ELISA and WB, conclusively affirming its ability to effectively react with the human CDK1/CDK2/CDK protein phosphorylated at T14.
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