Phospho-MAPK8/MAPK9/MAPK10 (T183/T183/T221) Antibody

Code CSB-RA013466A183phHU
Size US$350Purchase it in Cusabio online store
(only available for customers from the US)
Image
  • Western Blot
    Positive WB detected in 293 whole cell lysate(treated with EGF or not)
    All lanes Phospho-MAPK8/MAPK9/MAPK10 antibody at 1.65μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 46,54 KDa
    Observed band size: 46,54 KDa

  • Immunocytochemistry analysis of CSB-RA013466A183phHU diluted at 1:165 and staining in Hela cells(treated with 100ng/ml EGF for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

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Product Details

Uniprot No. P45983 P45984 P53779
Target Names MAPK8/MAPK9/MAPK10
Species Reactivity Human
Immunogen A synthesized peptide derived from Human Phospho-MAPK8/MAPK9/MAPK10 (T183/T183/T221)
Conjugate Non-conjugated
Clonality Monoclonal
Isotype Rabbit IgG
Clone No. 1A9
Purification Method Affinity-chromatography
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form Liquid
Tested Applications ELISA, WB, IHC
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:50-1:200
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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