Phospho-MAPK8/MAPK9/MAPK10 (T183/T183/T221) Recombinant Monoclonal Antibody

Code CSB-RA013466A183phHU
Size US$210
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  • Western Blot
    Positive WB detected in 293 whole cell lysate(treated with EGF or not)
    All lanes Phospho-MAPK8/MAPK9/MAPK10 antibody at 1.65μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 46,54 KDa
    Observed band size: 46,54 KDa
  • Immunocytochemistry analysis of CSB-RA013466A183phHU diluted at 1:165 and staining in Hela cells(treated with 100ng/ml EGF for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Product Details

Uniprot No.
Target Names
MAPK8/MAPK9/MAPK10
Species Reactivity
Human
Immunogen
A synthesized peptide derived from Human Phospho-MAPK8/MAPK9/MAPK10 (T183/T183/T221)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone No.
1A9
Purification Method
Affinity-chromatography
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
Liquid
Tested Applications
ELISA, WB, IHC
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:50-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

In the process of generating the phospho-MAPK8/MAPK9/MAPK10 (T183/T183/T221) recombinant monoclonal antibody, the initial step involves the isolation of genes responsible for encoding the MAPK8/MAPK9/MAPK10 (T183/T183/T221) antibody. These genes are sourced from rabbits that have been previously immunized with a synthesized peptide derived from the human phospho-MAPK8/MAPK9/MAPK10 (T183/T183/T221) protein. Subsequently, these antibody genes are cloned into specialized expression vectors. Following this genetic modification, the vectors are skillfully introduced into mammalian suspension cells. These mammalian cells are then cultured, providing an environment conducive to the production and secretion of the antibodies. Moving forward, the phospho-MAPK8/MAPK9/MAPK10 (T183/T183/T221) recombinant monoclonal antibody undergoes a meticulous purification process that hinges on the principles of affinity chromatography, effectively separating the antibody from the surrounding cell culture supernatant. Lastly, the antibody's functionality is subjected to a comprehensive evaluation, spanning a diverse array of tests including ELISA, WB, and IHC, thereby confirming its capability to effectively interact with the human phospho-MAPK8/MAPK9/MAPK10 (T183/T183/T221) protein.

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