Code | CSB-RA013466A183phHU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
WB | 1:500-1:5000 |
IHC | 1:50-1:200 |
In the process of generating the phospho-MAPK8/MAPK9/MAPK10 (T183/T183/T221) recombinant monoclonal antibody, the initial step involves the isolation of genes responsible for encoding the MAPK8/MAPK9/MAPK10 (T183/T183/T221) antibody. These genes are sourced from rabbits that have been previously immunized with a synthesized peptide derived from the human phospho-MAPK8/MAPK9/MAPK10 (T183/T183/T221) protein. Subsequently, these antibody genes are cloned into specialized expression vectors. Following this genetic modification, the vectors are skillfully introduced into mammalian suspension cells. These mammalian cells are then cultured, providing an environment conducive to the production and secretion of the antibodies. Moving forward, the phospho-MAPK8/MAPK9/MAPK10 (T183/T183/T221) recombinant monoclonal antibody undergoes a meticulous purification process that hinges on the principles of affinity chromatography, effectively separating the antibody from the surrounding cell culture supernatant. Lastly, the antibody's functionality is subjected to a comprehensive evaluation, spanning a diverse array of tests including ELISA, WB, and IHC, thereby confirming its capability to effectively interact with the human phospho-MAPK8/MAPK9/MAPK10 (T183/T183/T221) protein.
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