Product Details

Uniprot No.

Target Names

Alternative Names

60S acidic ribosomal protein P1 antibody; AA409079 antibody; AI325195 antibody; AU020965 antibody; ik:tdsubc_2g1 antibody; M(2)21C antibody; MGC137236 antibody; OTTHUMP00000004008 antibody; p32 antibody; p34 antibody; RCJMB04_6d17 replication protein A2; 32kDa antibody; REPA2 antibody; Replication factor A protein 2 antibody; Replication protein A 32 kDa subunit antibody; Replication protein A 32kDa subunit antibody; Replication protein A 34 kDa subunit antibody; Replication protein A antibody; Replication Protein A2 (32kDa) antibody; Replication protein A2 antibody; Replication protein A2; 32kDa antibody; RF-A protein 2 antibody; Rf-A2 antibody; RFA antibody; RFA2_HUMAN antibody; RP-A p32 antibody; RP-A p34 antibody; RP21C antibody; RPA 2 antibody; RPA 32 antibody; RPA antibody; Rpa2 antibody; RPA32 antibody; RPA34 antibody; RpLP1 antibody; RpP2 antibody; xx:tdsubc_2g1 antibody; zgc:109822 antibody

Species Reactivity

Human

Immunogen

A synthesized peptide derived from Human Phospho-RPA2 (T21)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

3B2

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IF

Recommended Dilution

Application	Recommended Dilution
IF	1:20-1:200

Protocols

ELISA Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Replication Protein A2 serves as an essential component of the heterotrimeric RPA complex, playing a central role in DNA replication, repair, and recombination. Phosphorylation at threonine 21 represents a critical regulatory modification that occurs in response to DNA damage and replication stress, making phospho-RPA2 (T21) a valuable marker for studying checkpoint signaling pathways and the cellular response to genotoxic stress. Researchers investigating DNA damage response mechanisms, cell cycle regulation, and genome stability rely on precise detection of this phosphorylation event to understand how cells coordinate repair processes.

This recombinant monoclonal antibody, clone 3B2, offers the reproducibility and consistency that phosphorylation studies demand. Because recombinant antibodies are produced from defined genetic sequences, researchers can expect uniform performance across experiments and over time, eliminating the lot-to-lot variability that can complicate longitudinal studies or multi-site collaborations. The rabbit IgG format, raised against a synthetic phosphopeptide corresponding to the human phospho-T21 site, provides high specificity for the phosphorylated epitope.

Validation in immunofluorescence applications demonstrates clear nuclear localization in HeLa cells, consistent with RPA2's known function in DNA metabolism. The recommended working dilution range of 1:20 to 1:200 for immunofluorescence provides flexibility for optimization across different experimental conditions and detection systems. The antibody has also been validated for ELISA applications, supporting quantitative approaches to phospho-RPA2 detection.

For researchers exploring epigenetics, nuclear signaling, and DNA damage response pathways, this antibody provides a reliable tool for monitoring RPA2 phosphorylation dynamics in human samples, supporting investigations into replication stress, checkpoint activation, and therapeutic responses to DNA-damaging agents.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Related Products

Target Background

Function

As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Plays also a role in base excision repair (BER) probably through interaction with UNG. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance.

Gene References into Functions

RPA, best known for its role in DNA replication and repair, recruits HIRA to promoters and enhancers and regulates deposition of newly synthesized H3.3 to these regulatory elements for gene regulation. PMID: 28107649
Single point mutations in the RPA32 subunit of RPA that abolish interaction with RFWD3 also inhibit interstrand crossling repair, demonstrating that RPA-mediated RFWD3 recruitment to stalled replication forks is important for ICL repair. PMID: 28575657
The authors show that Vpr can form a trimolecular complex with UNG2 and RPA32 and the positive effect of UNG2 and RPA32 on the reverse transcription process leading to optimal virus replication and dissemination between the primary target cells of HIV-1. PMID: 27068393
RPA32 phosphorylation regulates replication arrest, recombination, late origin firing, and mitotic catastrophe PMID: 24819595
Expression of mutant RPA2 or loss of PALB2 expression led to significant DNA damage after replication stress, a defect accentuated by poly-ADP (adenosine diphosphate) ribose polymerase inhibitors. PMID: 25113031
Conserved motifs are required for RPA32 binding the the N-terminus of SMARCAL1. PMID: 24910198
study reports the characterization of the RPA32C-SMARCAL1 interface at the molecular level; implications of results are discussed with respect to the recruitment of SMARCAL1 and other DNA damage response and repair proteins to stalled replication forks PMID: 24730652
study concludes RPA2 expression is translationally regulated via internal ribosome entry site and by eIF3a and that this regulation is partly accountable for cellular response to DNA damage and survival. PMID: 23393223
this study has explored the role of RPA32 phosphorylation at CDK and ATR sites and propose that phosphorylation of the RPA32 subunit is dispensable for checkpoint activation induced by replication stress with aphidicolin. PMID: 23047005
4E-BP3 regulates eIF4E-mediated nuclear mRNA export and interacts with replication protein A2 PMID: 22684010
Data show that the R88C variant impairs binding of the R88C variant impairs binding of uracil-DNA glycosylase UNG2 to replication protein A RPA2. PMID: 22521144
Replication protein A1, replication protein A2, and cyclins D2 and D3 seem to have a parallel role in the promotion of cell cycle in astrocytic tumors being implicated in the malignant progression of these neoplasms. PMID: 21496876
RPA2 up-regulation may be involved in the growth and/or survival of BRCA1 tumor cells and useful in immunohistochemical discrimination of triple-negative BRCA1 tumors. PMID: 21137066
At the subunit level, 13 proteins out of 30 examined may interact with RPA2. PMID: 20679368
data suggest that RPA2 hyperphosphorylation plays a critical role in maintenance of genomic stability and cell survival after a DNA replication block via promotion of homologus recombination PMID: 20130019
Data suggest that PP4-mediated dephosphorylation of RPA2 is necessary for an efficient DNA-damage response. PMID: 20154705
RPA32, critical for cell proliferation and maintenance of genome stability, are markedly down-regulated, Data hypothesized that their DNA-related functions could be partially limited in TRAIL-resistant HL-60 cells. PMID: 19834905
Phosphorylation of the RPA2 subunit is observed after exposure of cells to ionizing radiation (IR) and other DNA-damaging agents, which implicates the modified protein in the regulation of DNA replication after DNA damage or in DNA repair. PMID: 11731442
RPA2 binds to menin and has a role in multiple endocrine neoplasia PMID: 12509449
C-terminal domain of hRPA32 subunit (RPA32C) facilitates initiation of SV40 replication. PMID: 15793585
in response to UV-induced DNA damage, ATR rapidly phosphorylates RPA2, disrupting its association with replication centers in the S-phase and contributing to the inhibition of DNA replication PMID: 17035231
Determination at single-nucleotide resolution the relative positions of the single-stranded DNA with interacting intrinsic tryptophans of RPA32. PMID: 17583916
RPA phosphorylation facilitates chromosomal DNA repair. PMID: 17928296
RPA32 is extensively phosphorylated after the induction of EBV lytic replication. Rad51 and RPA32 are necessary for the completion of EBV lytic infection. PMID: 19386720
The N-terminus of RPA1 and phosphorylation of RPA2 regulate RPA interactions with the MRE11-RAD50-NBS1 (MRN) complex and are important in the response to DNA damage. PMID: 19586055
mitotic phosphorylation of RPA2 starts at the onset of mitosis, and dephosphorylation occurs during late cytokinesis. PMID: 19671522

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Subcellular Location

Nucleus. Nucleus, PML body.

Protein Families

Replication factor A protein 2 family

Database Links

HGNC: 10290

OMIM: 179836

KEGG: hsa:6118

STRING: 9606.ENSP00000363021

UniGene: Hs.79411

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Code	CSB-RA020089A21phHU
Size	US$210
	Order now
Image	Immunofluorescence staining of Hela cells with CSB-RA020089A21phHU at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
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Phospho-RPA2 (T21) Recombinant Monoclonal Antibody

Product Details

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Target Background