Code | CSB-RA020089A21phHU |
Size | US$210 |
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Application | Recommended Dilution |
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IF | 1:20-1:200 |
CUSABIO cloned the DNA sequence encoding the phospho-RPA2 (T21) monoclonal antibody into the plasmid and then transfected into the cell line for expression. The monoclonal antibody was produced by immunizing the animals with the phosphopeptide corresponding to the residues surrounding Thr 21 of human RPA2. The product was purified through the affinity-chromatography method and then got the recombinant phospho-RPA2 (T21) monoclonal antibody. It belongs to the rabbit IgG. This phospho-RPA2 (T21) antibody can be used to detect human pT21-RPA2 protein in ELISA and IF applications.
RPA2 is a subunit of RPA, which is a heterotrimeric protein complex that specifically binds to ssDNA. RPA is essential for DNA replication initiation and replication elongation. When the ATR-dependent phosphorylation sites in RPA2 are mutated, the down-regulation of DNA synthesis after UV radiation is disrupted, but ATR activation is unaffected. The UV-induced, ATR-mediated inhibition of DNA replication is specifically required for Thr 21 and Ser 33, two residues among numerous phosphorylation sites in the amino terminus of RPA2.
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