Product Details

Uniprot No.

Target Names

RACGAP1

Alternative Names

CYK4 antibody; GAP antibody; Gap1 antibody; GTPase activating protein antibody; HsCYK-4 antibody; ID GAP antibody; KIAA1478 antibody; Male germ cell RacGap antibody; MgcRacGAP antibody; Protein CYK4 homolg antibody; Protein CYK4 homolog antibody; Rac GTPase activating protein 1 antibody; Rac GTPase-activating protein 1 antibody; RACGAP 1 antibody; Racgap1 antibody; RGAP1_HUMAN antibody

Species Reactivity

Human

Immunogen

A synthesized peptide from human RACGAP1 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

8G12

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IF, FC

Recommended Dilution

Application	Recommended Dilution
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

RACGAP1, also known as MgcRacGAP or CYK4, serves as a critical regulator of cytokinesis and cell division, functioning as a GTPase-activating protein that controls Rac and Cdc42 signaling pathways. This protein plays essential roles in central spindle assembly and the completion of cell division, making it a key target for researchers investigating cell cycle regulation, mitotic progression, and the molecular mechanisms underlying cellular proliferation.

This recombinant monoclonal antibody, clone 8G12, offers the reproducibility and consistency that demanding cell biology research requires. Generated against a synthetic peptide derived from human RACGAP1, the antibody is produced using recombinant technology, ensuring sequence-defined specificity and eliminating the lot-to-lot variability that can compromise longitudinal studies. The rabbit IgG format, combined with affinity chromatography purification, delivers a reagent optimized for reliable target detection in human samples.

Validation studies demonstrate strong performance across multiple experimental platforms. Immunofluorescence analysis in HepG2 cells reveals clear cellular localization patterns when used at dilutions between 1:50 and 1:200, with DAPI counterstaining enabling precise subcellular context. Flow cytometry validation in HeLa cells confirms robust detection with distinct positive signal separation from isotype controls, supporting quantitative analysis of RACGAP1 expression at the single-cell level. The antibody is also validated for ELISA applications, providing flexibility across different experimental workflows.

For researchers studying cytokinesis, cell division machinery, or exploring RACGAP1's emerging roles in cancer biology, this antibody provides a dependable tool backed by demonstrated performance in well-characterized human cell line models.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Related Products

Target Background

Function

Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Required for proper attachment of the midbody to the cell membrane during cytokinesis. Plays key roles in controlling cell growth and differentiation of hematopoietic cells through mechanisms other than regulating Rac GTPase activity. Also involved in the regulation of growth-related processes in adipocytes and myoblasts. May be involved in regulating spermatogenesis and in the RACGAP1 pathway in neuronal proliferation. Shows strong GAP (GTPase activation) activity towards CDC42 and RAC1 and less towards RHOA. Essential for the early stages of embryogenesis. May play a role in regulating cortical activity through RHOA during cytokinesis. May participate in the regulation of sulfate transport in male germ cells.

Gene References into Functions

RACGAP1 promotes proliferation of hepatocellular carcinoma cells by reducing activation of the Hippo and YAP pathways and promoting cytokinesis in coordination with nuclear basket protein, TPR. PMID: 30009820
The rs615382 variant disrupts the recombination signal binding protein with immunoglobulin kappa J binding site in Rac GTPase activating protein 1. Disruption of notch 1 mediated-repression of RACGAP1 may contribute to Prostate Cancer in African Americans. PMID: 29695400
Study showed that a subset of epithelial ovarian cancer (EOC) patients have RacGAP1 overexpression which is associated with advanced tumor stages and poor clinical outcomes. Also, RacGAP1 can positively regulate the activation of RhoA and Erk proteins, thus up-enhance the migration and invasion process of EOC. PMID: 29095547
Data show that comparing with Ki-67 and TOP2A, RacGAP1 allowed for a clearer prognostic statement. PMID: 27259241
RACGAP1 promotes the metastatic phenotype in uterine carcinosarcoma via a STAT3/survivin signaling pathway. PMID: 27121792
High RACGAP1 expression is associated with Basal-like Breast Cancers. PMID: 27216196
This study showed that the overexpressions of Ki67, RacGAP1, and TOP2a affect the prognosis of female breast cancer patients adversely PMID: 27284123
Data confirmed a strong correlation of AURKA and Wnt-modulator RACGAP1 gene expression both in the gastric tumor, the tumor-adjacent and the tumor-distant mucosa. PMID: 26778597
RacGAP1 Is a Novel Downstream Effector of E2F7-Dependent Resistance to Doxorubicin and Is Prognostic for Overall Survival in Squamous Cell Carcinoma PMID: 26018753
RACGAP1 expression levels in the nucleus and cytoplasm, determined by immunohistochemical staining, predict opposite clinical outcomes and that both could be independent prognostic markers for colorectal cancer. PMID: 26508373
central spindle assembly and 2 Plk1-dependent phosphorylations are required to establish efficient binding of the Ect2 BRCT in early cytokinesis. PMID: 25486482
RacGAP1 expression at the invasive front in gastric cancer was significantly correlated with factors reflecting tumor progression and poor prognosis.RACGAP1 gene expression in diffuse type gastric cancer was elevated. PMID: 24615626
RacGAP1 mediated endothelial barrier function loss and melanoma transmigration in a focal adhesion-dependent manner.RacGAP1 activation mediates focal adhesion assembly and actin stress fiber formation. PMID: 25475728
High RacGAP1 expression was an independent predictive marker for lymph node metastasis, recurrence and poor prognosis in colorectal cancer patients. PMID: 25568185
HCV viral protein NS5B polymerase activity was significantly reduced by silencing RacGAP1 and, vice versa, was increased by overexpression of RacGAP1 in a cell-based reporter assay. PMID: 25305482
We show that some are new substrates of the anaphase-promoting complex/cyclosome and validate KIFC1 and RacGAP1/Cyk4 as two such targets involved respectively in timely mitotic spindle disassembly and cell spreading PMID: 24857844
MgcRacGAP colocalizes with CGN and CGNL1 at TJs and forms a complex and interacts directly in vitro with CGN and CGNL1. PMID: 24807907
High expression of RACGAP1 is associated with meningiomas. PMID: 23525949
APC(CDH1) targets MgcRacGAP for destruction in the late M phase. PMID: 23696789
The crystal structure of the GAP domain of MgcRacGAP has been determined at a resolution of 1.9A; MgcRacGAP (residues 348-546) exists as a monomer in solution. PMID: 23665020
In untreated patients, Ki67, TOP2A, and RacGAP1 are significant and independent prognostic markers. PMID: 23135572
High RACGAP1 mRNA expression (above the median) was associated with poor disease-free survival and overall survival in high risk early breast cancer PMID: 23096218
MKlp1-CYK4 centralspindlin complex is a guanosine triphosphatase-activating protein (GAP) for Rac1 and not RhoA. PMID: 22945935
several Rho family small GTPases activate PI3K by an indirect cooperative positive feedback that required a combination of Rac, CDC42, and RhoG small GTPase activities PMID: 22683270
the level of RACGAP1 expression is sufficient to predict the early recurrence of HCC. PMID: 21825042
The plexin-B1/Rac interaction inhibits PAK activation and enhances Sema4D ligand binding PMID: 11937491
This enzyme is down-regulated by estradiol. PMID: 12493759
Expression of a GTPase-activating protein (GAP)-deficient mutant (R386A) of MgcRacGAP induces abnormal cortical activity during cytokinesis in U2OS cells. Multiple large blebs were observed in cells expressing MgcRacGAP R386A. PMID: 14729465
data reveal an important role for MgcRacGAP in STAT3 activation, and demonstrate that MgcRacGAP regulates IL-6-induced cellular differentiation in which STAT3 plays a pivotal role PMID: 15284113
MgcRacGAP regulates the activation and function of Cdc42 in mitosis. PMID: 15642749
H-Ras-specific activation of Rac-MKK3/6-p38 pathway has a role in invasion and migration of breast epithelial cells PMID: 15677464
intracellular Ca2+ is mobilized by sphingosine 1 phosphate and mediates rac activation and adherens junction assembly in endothelial cells PMID: 15728185
beta 2 chimerin has a role in rac-GAP-dependent inhibition of breast cancer cell proliferation PMID: 15863513
activation of Vav1-Rac signaling pathway by CXCL12 represents an important inside-out event controlling efficient up-regulation of alpha4beta1-dependent T lymphocyte adhesion PMID: 15872091
Cdk1 inactivation is sufficient to activate a signaling pathway leading to cytokinesis, which emanates from mitotic spindles and is regulated by ECT2, MgcRacGAP, and RhoA PMID: 16118207
MgcRacGAP controls the initiation of cytokinesis by regulating ECT2, which in turn induces the assembly of the contractile ring and triggers the ingression of the cleavage furrow PMID: 16129829
This study defines a cellular mechanism that links centralspindlin to Cep55, which, in turn, controls the midbody structure and membrane fusion at the terminal stage of cytokinesis. PMID: 16790497
These findings demonstrate that HIF-1alpha function is negatively affected by its interaction with MgcRacGAP. PMID: 17982282
report here that (i) MgcRacGAP is phosphorylated by Aurora B and Cdk1, (ii) PP2A dephosphorylates Aurora B and Cdk1 phosphorylated sites and (iii) inhibition of PP2A abrogates MgcRacGAP/Ect2 interaction PMID: 18201571
Study demonstrates that FIP3 and ECT2 form mutually exclusive complexes with Cyk-4 and that dissociation of ECT2 from the mid-body at late telophase may be required for the recruitment of FIP3 and recycling endosomes to the cleavage furrow. PMID: 18511905
study reports that mitotic complex genes Ect2, RacGAP, and MKLP1 are coordinately induced in S phase in proliferating T lymphocytes as well as in epithelial cells, depending upon activity of the CUX1 and E2F1 transcription factors PMID: 19015243
MKLP1 interacts with CYK4, a GTPase activating protein for the Rho GTPase, to form the centralspindlin complex. Centralspindlin is a key regulator of Rho function and microtubule organisation during cytokinesis, and is essential for cell division. PMID: 11782313

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Subcellular Location

Nucleus. Cytoplasm. Cytoplasm, cytoskeleton, spindle. Cytoplasmic vesicle, secretory vesicle, acrosome. Cleavage furrow. Midbody, Midbody ring. Cell membrane; Peripheral membrane protein; Cytoplasmic side.

Tissue Specificity

Highly expressed in testis, thymus and placenta. Expressed at lower levels in spleen and peripheral blood lymphocytes. In testis, expression is restricted to germ cells with the highest levels of expression found in spermatocytes. Expression is regulated

Database Links

HGNC: 9804

OMIM: 604980

KEGG: hsa:29127

STRING: 9606.ENSP00000309871

UniGene: Hs.505469

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Code	CSB-RA189952A0HU
Size	US$210
	Order now
Image	Immunofluorescence staining of HepG2 cell with CSB-RA189952A0HU at 1:50 , counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing Hela cells stained with CSB-RA189952A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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RACGAP1 Recombinant Monoclonal Antibody

Product Details

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Target Background