RANBP3 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA831539A0HU
Size	US$210
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Image	IHC image of CSB-RA831539A0HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of A-431 cell with CSB-RA831539A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing HepG2 cells stained with CSB-RA831539A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Target Background

Product Details

Uniprot No.

Q9H6Z4

Target Names

RANBP3

Alternative Names

DKFZp586I1520 antibody; Ran binding protein 3 antibody; Ran-binding protein 3 antibody; RANB3_HUMAN antibody; RanBP3 antibody

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human RANBP3

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

4B3

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

RANBP3 (Ran-binding protein 3) functions as a cofactor in the nuclear export pathway, modulating the activity of the exportin CRM1 and influencing the transport of proteins and RNA across the nuclear envelope. This regulatory role positions RANBP3 at the intersection of nucleocytoplasmic transport, cell signaling, and gene expression control, making it a protein of considerable interest in studies of cellular homeostasis, cancer biology, and developmental processes.

This recombinant monoclonal antibody, generated in rabbit against a synthetic peptide derived from human RANBP3, offers the reproducibility and consistency that demanding experimental workflows require. As a sequence-defined recombinant clone (4B3), it eliminates the lot-to-lot variability inherent in traditional hybridoma-derived antibodies, ensuring that your results remain comparable across extended studies and collaborative projects.

Validation across multiple platforms demonstrates the versatility of this antibody in diverse experimental contexts. Immunohistochemistry performed on paraffin-embedded human testis tissue using a Leica Bond system with citrate buffer antigen retrieval shows clear target detection at 1:100 dilution. Immunofluorescence studies in A-431 cells reveal distinct staining patterns when counterstained with DAPI, while flow cytometry analysis of HepG2 cells demonstrates a clear positive shift compared to isotype control, confirming reliable detection in both adherent epithelial and hepatocellular carcinoma cell models.

Whether investigating nuclear transport mechanisms, exploring RANBP3's emerging connections to TGF-beta and Wnt signaling pathways, or characterizing its expression patterns in human tissues, this antibody provides a dependable tool for immunohistochemistry, immunofluorescence, and flow cytometry applications in your research.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Acts as a cofactor for XPO1/CRM1-mediated nuclear export, perhaps as export complex scaffolding protein. Bound to XPO1/CRM1, stabilizes the XPO1/CRM1-cargo interaction. In the absence of Ran-bound GTP prevents binding of XPO1/CRM1 to the nuclear pore complex. Binds to CHC1/RCC1 and increases the guanine nucleotide exchange activity of CHC1/RCC1. Recruits XPO1/CRM1 to CHC1/RCC1 in a Ran-dependent manner. Negative regulator of TGF-beta signaling through interaction with the R-SMAD proteins, SMAD2 and SMAD3, and mediating their nuclear export.

Gene References into Functions

Computational docking simulation indicates that phosphorylation of RanBP3 at Ser58 could potentially stabilize the association of RanBP3 with importin-alpha through interactions between the phosphate moiety of phospho-Ser58 of RanBP3 and a cluster of basic residues (Arg96 and Lys97 in importin-alpha3) on armadillo repeat 1 of importin-alpha. PMID: 28760339
RANBP3 regulates melanoma cell proliferation and probably a potential target for molecular targeted therapy. PMID: 26763446
RanBP3 is involved in vRNP nuclear export. The function of RanBP3 during vRNP nuclear export is regulated by phosphorylation at Ser58. RanBP3 phosphorylation is modulated by both PI3K/Akt and Ras/ERK/RSK pathways in the late phase of viral infection. PMID: 23303829
Crystal structure of the Ran-binding domain (RBD) from RanBP3 and compare it to RBD structures from RanBP1 and RanBP2 in complex with Ran and CRM1. PMID: 21364925
RanBP3 stimulates export by enhancing the affinity of Crm1 for Ran-GTP and cargo. PMID: 11932251
A multifunctional domain in CRM1 (exportin 1) mediates RanBP3 binding and multimerization of human T-cell leukemia virus type 1 Rex protein. PMID: 14612415
RANBP3 phosphorylation links the Ras and PI3-kinase pathways to nucleocytoplasmic transport. PMID: 18280241
RanBP3 directly recognizes dephosphorylated Smad2/3, which results from the activity of nuclear Smad phosphatases, and mediates nuclear export of Smad2/3 in a Ran-dependent manner. PMID: 19289081

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Subcellular Location

Cytoplasm. Nucleus.

Tissue Specificity

Widely expressed with high levels in testis and heart.

Database Links

HGNC: 9850

OMIM: 603327

KEGG: hsa:8498

STRING: 9606.ENSP00000341483

UniGene: Hs.531752

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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