RANGAP1 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA922439A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: Ntera-2 whole cell lysate(30µg),PC-3 whole cell lysate(30µg), Hela whole cell lysate(30µg),NIH/3T3 whole cell lysate(30µg) All lanes: RANGAP1 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 64 kDa Observed band size: 64,90 kDa Exposure time：2min IHC image of CSB-RA922439A0HU diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA922439A0HU diluted at 1:100 and staining in paraffin-embedded human stomach tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of K-562 cell with CSB-RA922439A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing Hela cells stained with CSB-RA922439A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Target Background

Product Details

Uniprot No.

P46060

Target Names

RANGAP1

Alternative Names

Fug 1 antibody; Fug1 antibody; GTPase-activating protein, RAN, 1 antibody; KIAA1835 antibody; MGC20266 antibody; OTTHUMP00000028918 antibody; OTTHUMP00000198755 antibody; OTTHUMP00000198756 antibody; OTTHUMP00000198757 antibody; OTTHUMP00000198758 antibody; RAGP1_HUMAN antibody; Ran 1 antibody; RAN GTPase activating protein 1 antibody; Ran GTPase-activating protein 1 antibody; Ran1 antibody; RANGAP 1 antibody; RANGAP antibody; RanGAP1 antibody; SD antibody; Segregation distorter homolog antibody; Segregation distortion antibody

Species Reactivity

Human, Mouse

Immunogen

A synthesized peptide derived from human RANGAP1

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

9E1

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

RANGAP1 plays a central role in regulating nucleocytoplasmic transport, functioning as the GTPase-activating protein for the small GTPase Ran. This regulatory activity is essential for maintaining the Ran-GTP gradient across the nuclear envelope, which drives the directional movement of proteins and RNA between the nucleus and cytoplasm. Disruption of RANGAP1 function has been implicated in various cellular processes including mitotic spindle assembly, nuclear envelope reformation, and chromosome segregation, making it a target of significant interest in cell biology and cancer research.

This recombinant monoclonal antibody, clone 9E1, offers researchers the reproducibility and consistency that comes with sequence-defined production. Unlike traditional hybridoma-derived antibodies, recombinant technology ensures that every lot performs identically, eliminating the variability that can compromise longitudinal studies or multi-site collaborations.

Validation across multiple experimental platforms demonstrates the versatility of this antibody for diverse research workflows. Western blot analysis detects RANGAP1 in human cell lines including Ntera-2, PC-3, and HeLa, as well as mouse NIH/3T3 cells, confirming cross-species reactivity between human and mouse samples. The observed bands at 64 kDa correspond to the predicted molecular weight, while an additional band at approximately 90 kDa likely reflects the SUMOylated form of RANGAP1, a well-characterized post-translational modification important for its localization at nuclear pore complexes. Immunohistochemistry has been validated in human glioma and stomach tissues, while immunofluorescence staining in K-562 cells reveals characteristic nuclear envelope and cytoplasmic localization patterns. Flow cytometry analysis in HeLa cells further extends its utility for quantitative single-cell studies.

This antibody serves researchers investigating nuclear transport mechanisms, cell cycle regulation, and cancer biology where RANGAP1 dysfunction may contribute to disease pathology.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

GTPase activator for RAN. Converts cytoplasmic GTP-bound RAN to GDP-bound RAN, which is essential for RAN-mediated nuclear import and export. Mediates dissociation of cargo from nuclear export complexes containing XPO1, RAN and RANBP2 after nuclear export.

Gene References into Functions

RanGAP1 upregulation is associated with drug resistance in Chronic Myeloid Leukemia. PMID: 27228340
Abnormal localization of RanGAP1 was found in cortex of Huntington's disease patients. PMID: 28384474
our results elucidate that RanGAP1 is actively transported between the nuclear and cytoplasmic compartments, and that the cytoplasmic and NPC localization of RanGAP1 is dependent on CRM1-mediated nuclear export. PMID: 26506250
immune cell adaptor SLP-76 binds directly to SUMO-RanGAP1 of cytoplasmic fibrils of the nuclear pore complex, and this interaction is needed for optimal NFATc1 and NF-kappaB p65 nuclear entry in T cells PMID: 26321253
Differentiation of human coronary artery smooth muscle cell to a contractile phenotype by stepwise serum depletion leads to significant reduction of RanGAP1 protein levels. PMID: 24988324
Analysis of the dynamics of E2(Ubc9)-SUMO-Target(RanGAP1) in the absence and presence of E3(RanBP2) revealed that two different allosteric sites regulate the ligase activity. PMID: 21216249
the RanGAP1 consensus sumoylation site and SUMO-1 C terminus are both conformationally flexible PMID: 15355965
the 3.0-A crystal structure of a four-protein complex of Ubc9, a Nup358/RanBP2 E3 ligase domain (IR1-M) and SUMO-1 conjugated to the carboxy-terminal domain of RanGAP1 PMID: 15931224
RanGAP1 is phosphorylated on Ser-358 in vivo & in vitro. Phosphorylated RanGAP1, but not a mutant at 358S, formed a stable ternary complex with Ran and RanBP1 in vivo, suggesting that its 358S phosphorylation affects the Ran system. PMID: 16428860
The results of this study strengthen the conclusion that mel-18 functions as an anti-SUMO E3 factor, and extend its targets to include regulation of the sumoylation of the important cellular protein RanGAP1. PMID: 18706886

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Subcellular Location

Cytoplasm. Nucleus, nucleoplasm. Nucleus envelope. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle.

Protein Families

RNA1 family

Tissue Specificity

Highly expressed in brain, thymus and testis.

Database Links

HGNC: 9854

OMIM: 602362

KEGG: hsa:5905

STRING: 9606.ENSP00000348577

UniGene: Hs.183800

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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