Product Details

Uniprot No.

Target Names

Alternative Names

KIAA0474 antibody; Rap1 GTPase activating protein 1 antibody; RAP1 GTPase activating protein antibody; Rap1 GTPase-activating protein 1 antibody; RAP1GA1 antibody; Rap1ga1 protein antibody; Rap1GAP antibody; Rap1GAP1 antibody; RAP1GAPII antibody; RAPGAP antibody; RPGP1_HUMAN antibody

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human RAP1GAP

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

12C12

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, FC

Recommended Dilution

Application	Recommended Dilution
FC	1:50-1:200

Protocols

ELISA Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

RAP1GAP serves as a critical regulator of Rap1 GTPase signaling, functioning to accelerate the hydrolysis of GTP-bound Rap1 and thereby modulating pathways essential for cell adhesion, migration, and proliferation. This regulatory role positions RAP1GAP as a significant target in cancer research, where altered Rap1 signaling has been implicated in tumor progression and metastatic behavior across multiple cancer types.

This recombinant monoclonal antibody, clone 12C12, offers researchers the reproducibility and consistency that sequence-defined antibody production provides. Generated against a synthetic peptide derived from human RAP1GAP and raised in rabbit, this antibody delivers the lot-to-lot reliability essential for longitudinal studies and multi-site collaborations where experimental consistency is paramount.

Validation through flow cytometry demonstrates robust detection of RAP1GAP in PC-3 human prostate cancer cells, a widely used model for studying aggressive prostate carcinoma. The flow cytometry protocol employed fixation and permeabilization to access intracellular RAP1GAP, with clear signal separation from isotype control at the recommended working dilution of 1:50 to 1:200. This validation in PC-3 cells is particularly relevant for researchers investigating Rap1 signaling dynamics in prostate cancer models.

The antibody is supplied in a stabilized liquid format with glycerol for convenient long-term storage at -20°C or -80°C, and the unconjugated format allows flexibility in secondary antibody and fluorophore selection to suit your detection system. For researchers exploring GTPase regulatory mechanisms, cell adhesion pathways, or cancer cell biology, this antibody provides a dependable tool for characterizing RAP1GAP expression and distribution in human samples.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

GTPase activator for the nuclear Ras-related regulatory protein RAP-1A (KREV-1), converting it to the putatively inactive GDP-bound state.

Gene References into Functions

This is the first mechanistic study of Rap1Gap in breast cancer progression. Decreases in Rap1Gap expression led to changes in adherens junctions via reduction in E-cadherin levels, to cytoskeletal remodeling, and to increases in ERK activation that are correlated with an invasive phenotype in DCIS PMID: 30144784
Rap1GAP may play a significant role in gastric cancer progression and act as a valuable prognostic marker for gastric cancer. PMID: 29758923
results have shown a novel role of PLCepsilon in the maintenance of endothelial barrier function, via its CDC25 GEF domain and lipase activity, and subsequent up-regulation of Rap1 activity PMID: 27612188
Findings suggest that RAP1 GTPase activating protein (Rap1GAP) is an important tumor suppressor with high prognostic value in endometrioid adenocarcinoma (EAC). PMID: 28196746
Rap1GAP is ubiquitinated and degraded through proteasome pathway in mitosis. Rap1GAP interacts with PLK1 in vivo. PMID: 25329897
kidney biopsies from glomerulosclerosis patients exhibited increased RAP1GAP, resulting in diminished glomerular RAP1 activation PMID: 24642466
findings identify Rap1GAP as a critical regulator of aggressive tumor cell behavior and suggest that the level of Rap1GAP expression influences the migratory mechanisms that are operative in tumor cells. PMID: 23864657
Results demonstrated that Rap1GAP promoted leukemia cell differentiation and apoptosis, but increased leukemia cell invasion in vitro. PMID: 22614916
Downregulation of RAP1GAP in thyroid tumors enhances SRC-dependent signals that regulate cellular architecture and motility. PMID: 22696507
Over-expression of Rap1GAP attenuated levels of both cadherins and integrins that are known to regulate the cancer cells invasion in renal cel carcinoma. PMID: 22266190
Rap1GAP is a more effective inhibitor of cell-matrix adhesion compared to cell-cell adhesion. PMID: 21785277
A polycomb-mediated repression of rap1GAP was demonstrated that involves EZH2, a histone methyltransferase in head and neck cancers. It was also shown that the loss of miR-101 expression correlates with EZH2 upregulation and rap1GAP downregulation. PMID: 21532618
These studies establish that the direct physical interaction of Rap1 with KRIT1 enables the translocation of microtubule-sequestered KRIT1 to junctions, thereby supporting junctional integrity and cardiovascular development PMID: 21633110
The expression level of RAP1GAp in myelodysplastic syndrome patients significantly increased as compared with patients with non-malignant blood diseases or AML. PMID: 19549374
Ca2+-dependent monomer and dimer formation switches CAPRI Protein between Ras GTPase-activating protein (GAP) and RapGAP activities PMID: 21460216
Results suggest that downregulation of Rap1GAP in epithelial tumors where alterations in cell/cell and cell/matrix adhesion are early steps in tumor dissemination supports a role for Rap1GAP depletion in tumor progression. PMID: 20439492
Epigenetic or genetic loss of Rap1GAP is very common in thyroid cancer, where these events are sufficient to promote cell proliferation and invasion. PMID: 20124489
Rap1GAP protein levels are tightly regulated and it may have a role as a tumor suppressor PMID: 14660640
data identify rap1GAP as a putative tumor suppressor gene in pancreatic cancer PMID: 16424023
Down-regulation of Rap1GAP is associated with oropharyngeal squamous cell carcinoma PMID: 16436672
Data implicate Rap1GAP as a putative tumor/invasion suppressor in the thyroid, and show that downregulation of Rap1GAP contributes to Ras transformation. PMID: 17646383
Rap1 increases KRIT-1 targeting to endothelial cell-cell junctions where it suppresses stress fibers and stabilizes junctional integrity. PMID: 17954608
Rap1GAP inhibits tumor growth but induces MMP2- and MMP9-mediated SCC invasion and tumor progression, suggesting a role for this protein as a biomarker for early N-stage, aggressive SCCs PMID: 18483282
Data propose that over-expression of RAP1GAP gene may play a role in the pathogenesis of myelodysplastic syndrome. PMID: 18551404
a role for Rap1GAP depletion in the progression of human thyroid tumors, possibly through unrestrained Rap activity. PMID: 19066305
Overexpression of Rap1GAP in melanoma cells blocks Rap1 activation and extracellular signal-regulated kinase (ERK) phosphorylation and inhibits melanoma cell proliferation and survival. PMID: 19147557
model in which the Ras GAP-related domain of GAP1(IP4BP) functions to stabilize the switch II region of Rap1, allowing stabilization of the transition state during GTP hydrolysis initiated by the arginine finger PMID: 19433443

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Subcellular Location

Golgi apparatus membrane; Peripheral membrane protein.

Tissue Specificity

Significant expression seen in the brain, kidney and pancreas. Abundant in the cerebral cortex and expressed at much lower levels in the spinal cord. Not detected in the lymphoid tissues.

Database Links

HGNC: 9858

OMIM: 600278

KEGG: hsa:5909

STRING: 9606.ENSP00000290101

UniGene: Hs.148178

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Code	CSB-RA559749A0HU
Size	US$210
	Order now
Image	Overlay Peak curve showing PC-3 cells stained with CSB-RA559749A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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RAP1GAP Recombinant Monoclonal Antibody

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