RAVER2 Recombinant Monoclonal Antibody

RAVER2, also known as Ribonucleoprotein PTB-binding 2, functions as a co-factor in the regulation of alternative splicing events mediated by polypyrimidine tract-binding proteins. This RNA-binding protein plays important roles in post-transcriptional gene regulation, making it a valuable target for researchers investigating splicing mechanisms, RNA processing, and gene expression control in various cellular contexts.

This recombinant monoclonal antibody, clone 4B7, offers the reproducibility and consistency that demanding experimental workflows require. Generated through recombinant technology with a defined sequence, this antibody eliminates the lot-to-lot variability inherent in traditional hybridoma production, ensuring your results remain comparable across extended studies and collaborative projects.

Validation data demonstrates robust performance across multiple experimental platforms. Western blot analysis confirms detection of RAVER2 in diverse human cell lines including HeLa, SH-SY5Y, 786-O, A549, HepG2, HT-29, and Jurkat cells, with an observed band at approximately 85 kDa. The difference from the predicted 74 kDa molecular weight likely reflects post-translational modifications such as glycosylation or phosphorylation events common to RNA-binding proteins. Immunofluorescence staining in SH-SY5Y cells reveals clear subcellular localization patterns, while flow cytometry analysis in HeLa cells shows distinct positive population shifts compared to isotype controls, confirming specificity for intracellular detection applications.

The antibody's validated performance in immunofluorescence and flow cytometry provides flexibility for researchers studying RAVER2 expression patterns, subcellular distribution, and protein levels across different experimental conditions. This reagent supports investigations into alternative splicing regulation, RNA metabolism, and the broader mechanisms of post-transcriptional control in human cell systems.