RBBP4 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA915915A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate, HEK293 whole cell lysate, L02 whole cell lysate, Jurkat whole cell lysate, SH-SY5Y whole cell lysate, Mouse Brain whole cell lysate, Rat Brain cell lysate All lanes: RbAp48 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 48, 48, 47, 44 kDa Observed band size: 53, 40 kDa IHC image of CSB-RA915915A0HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA915915A0HU diluted at 1:100 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of Hela Cells with CSB-RA915915A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Have Questions?	Leave a Message or Start an on-line Chat

Product Details
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Uniprot No.

Q09028

Target Names

RBBP4

Alternative Names

Histone-binding protein RBBP4 (Chromatin assembly factor 1 subunit C) (CAF-1 subunit C) (Chromatin assembly factor I p48 subunit) (CAF-I 48 kDa subunit) (CAF-I p48) (Nucleosome-remodeling factor subunit RBAP48) (Retinoblastoma-binding protein 4) (RBBP-4) (Retinoblastoma-binding protein p48), RBBP4, RBAP48

Species Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human RbAp48

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

3E9

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
IF	1:20-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

RBBP4, also known as RbAp48, serves as a critical component of multiple chromatin-modifying complexes that regulate gene expression, DNA repair, and cell cycle progression. As a core subunit of the NuRD complex, PRC2, and chromatin assembly factor 1, this histone-binding protein plays essential roles in epigenetic regulation and has emerged as a significant focus in cancer research due to its involvement in tumor suppressor pathways through its interaction with the retinoblastoma protein.

This recombinant monoclonal antibody, generated against a synthetic peptide derived from human RbAp48, offers the consistency and reproducibility that demanding epigenetic studies require. Because the antibody sequence is defined and production occurs in a controlled recombinant system, researchers can expect reliable performance across experiments and between lots, eliminating a common source of variability in chromatin biology workflows.

Validation across multiple experimental platforms demonstrates this antibody's versatility. Western blot analysis confirms robust detection in a broad panel of human cell lines including HeLa, K562, HepG2, HEK293, L02, Jurkat, and SH-SY5Y, with cross-species reactivity verified in mouse and rat brain lysates. The observed bands at approximately 53 kDa and 40 kDa compared to predicted sizes of 44–48 kDa likely reflect post-translational modifications and potential isoform detection. Immunohistochemistry validation in human breast cancer and lung tissue sections shows clear nuclear localization, while immunofluorescence staining in HeLa cells confirms expected nuclear distribution patterns.

Whether investigating chromatin remodeling mechanisms, exploring epigenetic dysregulation in cancer models, or examining nuclear signaling pathways, this antibody provides a reliable tool for researchers working across Western blot, immunohistochemistry, and immunofluorescence applications.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

■ Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function

Core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly following DNA replication and DNA repair; the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression; the nucleosome remodeling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling; the PRC2 complex, which promotes repression of homeotic genes during development; and the NURF (nucleosome remodeling factor) complex.

Gene References into Functions

ARMC12 promotes neuroblastoma progression through interaction with RBBP4. PMID: 30026490
BCL11A interacts with histone methyltransferase (PRC2) and histone deacetylase (NuRD and SIN3A) complexes through their common subunit, RBBP4/7. PMID: 29263092
RBAP48 overexpression contributes to the radiosensitivity of AGS gastric cancer cells via phosphoinositide3-kinase/protein kinase B pathway suppression. PMID: 29901205
The MTA1 subunit of the nucleosome remodeling and deacetylase complex can recruit two copies of RBBP4/RBBP7. PMID: 27144666
RbAp48 is likely to act as a potent antiretroviral defense. PMID: 27222146
The crystal structure reveals an extensive interface between MTA1 and RBBP4. PMID: 27098840
RBBP4 interacts with ep300 protein to form a complex that drives the expression of methylguanine-DNA-methyltransferase , RAD51, and other selected DNA repair genes through histone acetylation. PMID: 26972001
RbAp48 was identified as critical in the proliferation of hypopharyngeal carcinoma in both in vitro and in vivo experiments. PMID: 26376479
RBBP4 functions as a novel regulatory factor to increase the efficiency of importin alpha/beta-mediated nuclear import PMID: 26491019
It is associated with episodic memory performance. PMID: 25150575
Our RBBP4-PHF6 complex structure provides insights into the molecular basis of PHF6-NuRD complex interaction and implicates a role for PHF6 in chromatin structure modulation and gene regulation. PMID: 25601084
Our results reveal that the protein structure does not affect ligand binding, and the top three TCM candidates Bittersweet alkaloid II, Eicosandioic acid, and Perivine might resolve the instability of the RbAp48-FOG1 complex PMID: 25165715
RbAp48 recognizes MTA1 using the same site that it uses to bind histone H4, showing that assembly into NuRD modulates RbAp46/48 interactions with histones. PMID: 24920672
The most significant change was an age-related decline in RbAp48, a histone-binding protein that modifies histone acetylation. PMID: 23986399
study of the interaction of the histone H3-H4 complex with the RbAp48 and their exchange with a second histone chaperone, anti-silencing function protein 1 (ASF1); exchange of histones H3-H4 between these two histone chaperones has a central role in the assembly of new nucleosomes PMID: 23178455
Data identified RBBP4 and RBBP9 as required for maintenance of multiple PS cell types, and both RBBPs were bound to RB in PS cells. PMID: 21689726
The FOG-1 peptide contacts a negatively charged binding pocket on top of the RbAp48 beta-propeller that is distinct from the binding surface used by RpAp48 to contact histone H4 PMID: 21047798
Upergulation of CAF-1 p48 subunit by overexpression of tuberous sclerosis gene products. PMID: 12893285
expression of three (Beclin 1, RbAp48 and Pir51) were increased and one (aldolase b) was decreased in liver tumor tissues. PMID: 14966907
RbAp48 is a NF-kappaB-regulated gene playing an important role in thyroid cancer cell autonomous proliferation. PMID: 17244783
In conclusion, the data support a model whereby RbAp48 regulates cellular morphology and cytoskeletal organization by increasing K-Ras activity and signaling through MAPK. PMID: 17974974
Data show that RbAp46 and RbAp48 interact with estrogen receptor (ER)alpha at endogenous, estrogen-responsive genes and alter expression of endogenous, ERalpha-activated and -repressed genes in MCF-7 breast cancer cells. PMID: 18577416
Results report that transgenic expression of RbAp48 resulted in the development of autoimmune exocrinopathy resembling Sjogren's syndrome. PMID: 19015307

Hide All

Subcellular Location

Nucleus.

Protein Families

WD repeat RBAP46/RBAP48/MSI1 family

Database Links

HGNC: 9887

OMIM: 602923

KEGG: hsa:5928

STRING: 9606.ENSP00000362592

UniGene: Hs.16003

Promotions

Email: [email protected]
Distributors Worldwide

Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Cellular senescence

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

Join us with

Subscribe newsletter

Leave a message

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：
Please enter the characters shown. Click the image to refresh if unclear.

Place an order now

I. Product details

Product name *

Code *

Size *

Quantity *

Message

II. Contact details

Name *

Email address *

Company/Organization

Phone

III. Ship To

Address *

City *

State/Province *

Zip/Post code *

Country *

Attn Name *

Phone# *

IV. Bill To Same as Shipping Address