RBBP4 Recombinant Monoclonal Antibody

Code CSB-RA915915A0HU
Size US$210
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Image
  • Western Blot
    Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate, HEK293 whole cell lysate, L02 whole cell lysate, Jurkat whole cell lysate, SH-SY5Y whole cell lysate, Mouse Brain whole cell lysate, Rat Brain cell lysate
    All lanes: RbAp48 antibody at 1:1000
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 48, 48, 47, 44 kDa
    Observed band size: 53, 40 kDa
  • IHC image of CSB-RA915915A0HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
  • IHC image of CSB-RA915915A0HU diluted at 1:100 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
  • Immunofluorescence staining of Hela Cells with CSB-RA915915A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
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Product Details

Uniprot No.
Target Names
RBBP4
Alternative Names
Histone-binding protein RBBP4 (Chromatin assembly factor 1 subunit C) (CAF-1 subunit C) (Chromatin assembly factor I p48 subunit) (CAF-I 48 kDa subunit) (CAF-I p48) (Nucleosome-remodeling factor subunit RBAP48) (Retinoblastoma-binding protein 4) (RBBP-4) (Retinoblastoma-binding protein p48), RBBP4, RBAP48
Species Reactivity
Human, Mouse, Rat
Immunogen
A synthesized peptide derived from human RbAp48
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone No.
3E9
Purification Method
Affinity-chromatography
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
Liquid
Tested Applications
ELISA, WB, IHC, IF
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:50-1:200
IF 1:20-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

The process of producing an RBBP4 recombinant antibody involves four stages: firstly, sequencing the RBBP4 monoclonal antibody gene; secondly, inserting the gene into a plasmid vector; thirdly, introducing the recombinant vector into a host cell line; and fourthly, purifying the RBBP4 recombinant monoclonal antibody from the cell culture supernatant through affinity chromatography. The RBBP4 monoclonal antibody is created from hybridomas that produce RBBP4 antibodies, and a synthesized peptide derived from human RBBP4 is used as the immunogen during the production process. This recombinant RBBP4 monoclonal antibody is recommended for use in ELISA, WB, IHC, and IF applications to detect RBBP4 protein from human, mouse, and rat samples.RBBP4 is involved in epigenetic regulation, cell cycle regulation, DNA repair, and development.

RBBP4 is a component of several chromatin-modifying complexes, including the HDAC complex and the nucleosome remodeling, and the NuRD complex, which play important roles in the regulation of gene expression by modifying chromatin structure. As a key regulator of the G1/S transition of the cell cycle, RBBP4 interacts with the retinoblastoma tumor suppressor protein (RB) to promote cell cycle arrest in response to DNA damage or other stresses. RBBP4 plays a role in DNA double-strand break repair by interacting with the DNA repair protein RAD51. RBBP4 is involved in embryonic development, as knockout studies have shown that it is required for early embryonic development and is involved in the differentiation of various cell types.

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Target Background

Function
Core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly following DNA replication and DNA repair; the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression; the nucleosome remodeling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling; the PRC2 complex, which promotes repression of homeotic genes during development; and the NURF (nucleosome remodeling factor) complex.
Gene References into Functions
  1. ARMC12 promotes neuroblastoma progression through interaction with RBBP4. PMID: 30026490
  2. BCL11A interacts with histone methyltransferase (PRC2) and histone deacetylase (NuRD and SIN3A) complexes through their common subunit, RBBP4/7. PMID: 29263092
  3. RBAP48 overexpression contributes to the radiosensitivity of AGS gastric cancer cells via phosphoinositide3-kinase/protein kinase B pathway suppression. PMID: 29901205
  4. The MTA1 subunit of the nucleosome remodeling and deacetylase complex can recruit two copies of RBBP4/RBBP7. PMID: 27144666
  5. RbAp48 is likely to act as a potent antiretroviral defense. PMID: 27222146
  6. The crystal structure reveals an extensive interface between MTA1 and RBBP4. PMID: 27098840
  7. RBBP4 interacts with ep300 protein to form a complex that drives the expression of methylguanine-DNA-methyltransferase , RAD51, and other selected DNA repair genes through histone acetylation. PMID: 26972001
  8. RbAp48 was identified as critical in the proliferation of hypopharyngeal carcinoma in both in vitro and in vivo experiments. PMID: 26376479
  9. RBBP4 functions as a novel regulatory factor to increase the efficiency of importin alpha/beta-mediated nuclear import PMID: 26491019
  10. It is associated with episodic memory performance. PMID: 25150575
  11. Our RBBP4-PHF6 complex structure provides insights into the molecular basis of PHF6-NuRD complex interaction and implicates a role for PHF6 in chromatin structure modulation and gene regulation. PMID: 25601084
  12. Our results reveal that the protein structure does not affect ligand binding, and the top three TCM candidates Bittersweet alkaloid II, Eicosandioic acid, and Perivine might resolve the instability of the RbAp48-FOG1 complex PMID: 25165715
  13. RbAp48 recognizes MTA1 using the same site that it uses to bind histone H4, showing that assembly into NuRD modulates RbAp46/48 interactions with histones. PMID: 24920672
  14. The most significant change was an age-related decline in RbAp48, a histone-binding protein that modifies histone acetylation. PMID: 23986399
  15. study of the interaction of the histone H3-H4 complex with the RbAp48 and their exchange with a second histone chaperone, anti-silencing function protein 1 (ASF1); exchange of histones H3-H4 between these two histone chaperones has a central role in the assembly of new nucleosomes PMID: 23178455
  16. Data identified RBBP4 and RBBP9 as required for maintenance of multiple PS cell types, and both RBBPs were bound to RB in PS cells. PMID: 21689726
  17. The FOG-1 peptide contacts a negatively charged binding pocket on top of the RbAp48 beta-propeller that is distinct from the binding surface used by RpAp48 to contact histone H4 PMID: 21047798
  18. Upergulation of CAF-1 p48 subunit by overexpression of tuberous sclerosis gene products. PMID: 12893285
  19. expression of three (Beclin 1, RbAp48 and Pir51) were increased and one (aldolase b) was decreased in liver tumor tissues. PMID: 14966907
  20. RbAp48 is a NF-kappaB-regulated gene playing an important role in thyroid cancer cell autonomous proliferation. PMID: 17244783
  21. In conclusion, the data support a model whereby RbAp48 regulates cellular morphology and cytoskeletal organization by increasing K-Ras activity and signaling through MAPK. PMID: 17974974
  22. Data show that RbAp46 and RbAp48 interact with estrogen receptor (ER)alpha at endogenous, estrogen-responsive genes and alter expression of endogenous, ERalpha-activated and -repressed genes in MCF-7 breast cancer cells. PMID: 18577416
  23. Results report that transgenic expression of RbAp48 resulted in the development of autoimmune exocrinopathy resembling Sjogren's syndrome. PMID: 19015307

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Subcellular Location
Nucleus.
Protein Families
WD repeat RBAP46/RBAP48/MSI1 family
Database Links

HGNC: 9887

OMIM: 602923

KEGG: hsa:5928

STRING: 9606.ENSP00000362592

UniGene: Hs.16003

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