RCOR1 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA103529A0HU
Size	US$210
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Image	IHC image of CSB-RA103529A0HU diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Overlay Peak curve showing Hela cells stained with CSB-RA103529A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Product Details
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Target Background

Product Details

Uniprot No.

Q9UKL0

Target Names

RCOR1

Alternative Names

COREST antibody; KIAA0071 antibody; Nr2b2 antibody; Nuclear receptor coregulator 1 antibody; Nuclear receptor subfamily 2 group B member 2 antibody; Protein CoREST antibody; RCOR antibody; Rcor1 antibody; RCOR1_HUMAN antibody; REST corepressor 1 antibody; Retinoic acid receptor RXR-beta antibody; Retinoid X receptor beta antibody; Rxrb antibody

Species Reactivity

Human

Immunogen

A synthesized peptide from human RCOR1 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

2C10

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IHC, FC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

RCOR1, also known as CoREST, functions as a critical corepressor protein that partners with REST to silence neuronal genes in non-neuronal tissues. This transcriptional regulator plays a central role in chromatin remodeling by recruiting histone deacetylases and demethylases to target gene promoters, making it an essential component of epigenetic regulatory complexes. Researchers investigating neuronal differentiation, stem cell biology, and cancer epigenetics frequently rely on RCOR1 detection to understand how gene silencing programs are established and maintained.

This recombinant monoclonal antibody, clone 2C10, offers the reproducibility that demanding epigenetic studies require. Because the antibody sequence is defined and produced in a controlled recombinant system, you can expect consistent performance across experiments and between lots, eliminating a common source of variability in longitudinal studies or multi-site collaborations.

Validation data demonstrates reliable performance across complementary detection platforms. In immunohistochemistry, the antibody produces clear nuclear staining in paraffin-embedded human lung cancer tissue at dilutions between 1:50 and 1:200, with optimal results observed at 1:100 using citrate buffer antigen retrieval. Flow cytometry validation in HeLa cells confirms specific intracellular detection, showing a distinct positive shift compared to isotype control when cells are properly fixed and permeabilized. This dual-platform validation provides flexibility for researchers who need to examine RCOR1 expression in both tissue sections and cell populations.

Whether you are characterizing CoREST complex dynamics in cancer models, investigating chromatin state transitions during differentiation, or exploring nuclear signaling pathways, this antibody delivers the specificity and consistency needed for publication-quality results in epigenetics research.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Essential component of the BHC complex, a corepressor complex that represses transcription of neuron-specific genes in non-neuronal cells. The BHC complex is recruited at RE1/NRSE sites by REST and acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. In the BHC complex, it serves as a molecular beacon for the recruitment of molecular machinery, including MeCP2 and SUV39H1, that imposes silencing across a chromosomal interval. Plays a central role in demethylation of Lys-4 of histone H3 by promoting demethylase activity of KDM1A on core histones and nucleosomal substrates. It also protects KDM1A from the proteasome. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development and controls hematopoietic differentiation.

Gene References into Functions

the intact CoREST complex is targeted with dual histone deacetylase and demethylase inhibitors PMID: 29302039
Expressions of REST and RCOR1 genes may downregulate SYN1 expression in gliomas. PMID: 27685921
Rcor1 knock-out monocytes exhibited extensive self-renewal associated with hematopoietic stem cell expansion. PMID: 26119982
Genomic deletions in RCOR1 are associated with a specific gene expression signature and with unfavorable clinical outcomes in diffuse large B-cell lymphoma patients. PMID: 25395426
a combination of virtual screening and biological approaches can lead to compounds reducing REST complex formation PMID: 23800350
Data show that miR-22 specifically interacts with the 3' UTRs of the Rcor1, Rgs2 and HDAC4 mRNAs. PMID: 23349832
The CoREST and REST are necessary and inimical for expression of herpes simplex virus genes. PMID: 21221247
Insight into the molecular basis of the interaction between scaffolding protein CoREST and histone deacetylase LSD1 may suggest a new means of inhibiting LSD1 activity by misdirecting the enzyme away from nucleosomal substrates. PMID: 21142040
silencing of CoREST by siRNA transfection in normal articular cartilage may reflect CoREST repression in advanced osteoarthritic cartilage, which results in phenotypic genes modulation and suggests a homeostatic role of this transcription factor PMID: 20973059
CoREST does not play an influential role in regulating HSV-1 infection. PMID: 20106915
elevated levels of REST and CoREST in the brain of RTT patients and MeCP2 deficient mice result in downregulation of BDNF, apparently by their binding to the RE1 (element) located between the first two promoters of the BDNF gene PMID: 18075316
CoREST is bound to the hsp70 gene promoter under basal conditions and that its binding increases during heat shock response. PMID: 18657505
These results demonstrate that SUMO-1 modification modulates the transcriptional repression by CoREST and is needed for its full repressive activity. PMID: 18854179
The results indicate that both sets of events mediated by ICP0, the degradation of PML and the blocking of silencing via the HDAC1/2-CoREST-REST complex, are interdependent and in large measure dependent on events in the ND10 nuclear bodies. PMID: 18945770
Direct interactions between CoREST1 and SUMO-2 mediate SUMO-dependent changes in chromatin structure and transcription that are important for cell-type-specific gene expression. PMID: 19394292
Analysis of chromatin modification patterns shows that CoREST are recruited to the c-myc promoter leading to appearance of repressive chromatin marks. PMID: 19522008

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Subcellular Location

Nucleus. Note=Upon infection by HSV-1, it is partially translocated into the cytoplasm in an HSV-1-dependent manner.

Protein Families

CoREST family

Tissue Specificity

Ubiquitously expressed.

Database Links

HGNC: 17441

OMIM: 607675

KEGG: hsa:23186

STRING: 9606.ENSP00000459789

UniGene: Hs.510521

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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