Product Details

Uniprot No.

Q01201

Target Names

RELB

Alternative Names

Transcription factor RelB (I-Rel), RELB

Species Reactivity

Human

Immunogen

A synthesized peptide derived from Human RELB

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

9E4

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IF, FC

Recommended Dilution

Application	Recommended Dilution
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

RelB serves as a critical member of the NF-κB transcription factor family, playing a distinctive role in the non-canonical NF-κB signaling pathway. Unlike other NF-κB subunits, RelB specifically regulates genes involved in lymphoid organ development, dendritic cell maturation, and adaptive immune responses. Its dysregulation has been implicated in various malignancies and inflammatory conditions, making it a compelling target for researchers investigating cancer biology, immune signaling, and epigenetic regulation.

This recombinant monoclonal antibody, clone 9E4, offers the reproducibility and consistency that demanding experimental workflows require. Because recombinant antibodies are produced from defined genetic sequences rather than traditional hybridoma methods, researchers benefit from lot-to-lot uniformity that supports longitudinal studies and ensures comparable results across experiments. The rabbit IgG format provides high affinity binding, while affinity chromatography purification delivers a clean preparation suitable for sensitive detection methods.

Validation studies demonstrate reliable performance in both immunofluorescence and flow cytometry applications using HeLa cells. Immunofluorescence staining reveals clear RelB detection with nuclear localization patterns consistent with its function as a transcription factor, while flow cytometry analysis shows distinct positive population shifts compared to isotype controls. These validated applications provide flexibility for researchers examining RelB expression and localization in fixed and permeabilized human cell preparations.

Whether investigating NF-κB pathway dynamics in cancer models, characterizing immune cell differentiation, or exploring epigenetic regulatory networks, this antibody delivers the specificity and consistency needed for meaningful experimental outcomes. The unconjugated format allows pairing with the secondary detection system best suited to your specific instrumentation and multiplexing requirements.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Related Products

Target Background

Function

NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric RelB-p50 and RelB-p52 complexes are transcriptional activators. RELB neither associates with DNA nor with RELA/p65 or REL. Stimulates promoter activity in the presence of NFKB2/p49. As a member of the NUPR1/RELB/IER3 survival pathway, may provide pancreatic ductal adenocarcinoma with remarkable resistance to cell stress, such as starvation or gemcitabine treatment. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer in a CRY1/CRY2 independent manner. Increased repression of the heterodimer is seen in the presence of NFKB2/p52. Is required for both T and B lymphocyte maturation and function.

Gene References into Functions

Results show that GSK3beta modulates RelB degradation. PMID: 29358699
Data show that TNF receptor-associated factor 3 (TRAF3) autophagy is driven by RAS and results in activation of transcription factor RelB (RELB). PMID: 29146913
TNF-alpha-induced expression of transport protein genes in HUVEC cells is associated with enhanced expression of RELB and NFKB2. PMID: 29658079
results suggest that changes in the relative concentrations of RelB, NIK:IKK1, and p100 during noncanonical signaling modulate this transitional complex and are critical for maintaining the fine balance between the processing and protection of p100. PMID: 27678221
Low RELB expression is associated with Prostate Cancer. PMID: 28108513
RelB is processed by CO2 in a manner dependent on a key C-terminal domain located in its transactivation domain. Loss of the RelB transactivation domain alters NF-kappaB-dependent transcriptional activity, and loss of p100 alters sensitivity of RelB to CO2 PMID: 28507099
EZH2, through a methyltransferase-independent mechanism, promotes the transcriptional activation of the non-canonical NF-kappaB subunit RelB PMID: 27764181
The altered expression of anti-apoptotic gene Bcl-2 played critical roles in regulating both spontaneous and radiation-induced apoptosis in the presence of RelB knockdown. For the first time, we showed that RelB knockdown significantly attenuated the migration and invasion of DU145 prostate cancer cells, due to the reduction of integrin b-1 PMID: 27121503
Knockdown of ADGRG2 breast cancer cell lines resulted in a strong reduction in cell adhesion and subsequent cell migration which was associated with a selective reduction in RelB. PMID: 26321231
the role of RelB on lymphocyte development in humans was shown. PMID: 26385063
In conclusion, DECs show a unique hypo-responsive phenotype to the pro-inflammatory stimulus LPS in order to control the inflammatory response at feto-maternal interface. PMID: 26463648
Lung-specific (CC-16) and novel (RelB) biomarkers are associated with systemic cardiovascular changes over time. PMID: 26914709
results suggest that glucocorticoids induce a transcription complex consisting of RelB/p52, CBP, and HDAC1 that triggers a dynamic acetylation-mediated epigenetic change to induce CRH expression in full-term human placenta. PMID: 26307012
HDAC4-RelB-p52 complex maintains repressive chromatin around proapoptotic genes Bim and BMF and regulates multiple myeloma survival and growth PMID: 26455434
RELB enhances proliferation of human-induced pluripotent stem cells without affecting their pluripotency. RELB interacts with LIN28A and IMP3. PMID: 25794352
In non-small cell lung cancer, RelB expression was identified in proliferating tumor cells. tumor RelB expression was an independent predictor of lymph node metastasis PMID: 26147201
Basal expression of RelB was significantly lower in lung cells derived from smokers with and without COPD. PMID: 25943190
RelB may represent a novel marker of health outcomes PMID: 25409035
the specific Asp205 cleavage of RelB by caspase-3 would be involved in the apoptosis induction by anticancer agents, which would provide the positive feedback mechanism. PMID: 25511695
we demonstrate that SUMOylation of RelB might be one of these post-translational modifications rendering the function of the NF-kappaB transcription factor RelB. PMID: 24616021
RelB-p52 dimers were found to directly bind to the IDO promoter, leading to IDO expression in MDSCs PMID: 25063873
Unstimulated monocyte-derived dendritic cells express RelB at low level, However, RelB increases, following stimulation, are attenuated by geldanamycin. PMID: 24524692
CTNNA1 expression is specifically downregulated in basal-like breast cancer subtype, correlates with clinical outcome and inversely correlates with TNF and RELB expression. PMID: 24509793
The NF-kappaB protein RelB is expressed in a particularly aggressive mesenchymal subtype of glioma. PMID: 23451236
RelB activation is key for promoting multiple myeloma cell survival through the upregulation of anti-apoptotic proteins, in particular, CIAP2. PMID: 23555623
The dimer RelB/p50 rather than the p50/p50 complex inhibits TNF production in lipopolysaccharide-stimulated dendritic cells and macrophages. PMID: 23394901
a novel link between NF-kappaB and growth-inhibitory pathways involving the RelB-dependent transcriptional upregulation of p53. PMID: 22777360
Kaposi's sarcoma-associated herpesvirus oncoprotein K13 upregulated the expression of NF-kappaB subunit RelB and blocked the anti-IgM-induced decline in c-Myc and rise in p27(Kip1) that have been associated with growth arrest and apoptosis. PMID: 23236068
The expression of RelB negatively regulates the endogenous expression of maspin in prostate cancer cells in vitro. PMID: 22780967
The RelB subunit of NFkappaB acts as a negative regulator of circadian gene expression. PMID: 22894897
These data show that Hodgkin lymphoma is uniquely dependent on relB PMID: 22968463
RelB/NF-kappaB2, is constitutively activated in the human placenta, which binds to a previously undescribed NF-kappaB enhancer of corticotropin-releasing hormone (CRH) gene promoter to regulate CRH expression. PMID: 22734038
RelB is a CO(2)-sensitive NF-kappaB family member that may contribute to the beneficial effects of hypercapnia in inflammatory diseases of the lung PMID: 22396550
RelB plays a critical role in the response of PCa to radiotherapy and the inverse expression of IL-8 and PSA PMID: 22403723
We propose that RelB is an essential molecule controlling the endogenous and the proteasome inhibitor-induced Maspin expression. PMID: 21856005
a central role for Malt1-dependent RelB cleavage in canonical NF-kappaB activation and thereby provide a rationale for the targeting of Malt1 in immunomodulation and cancer treatment. PMID: 21873235
AHR overexpression is found among estrogen receptor (ER)alpha-negative human breast tumors and that its overexpression is positively correlated to that of the NF-kappaB subunit Rel-B and Interleukin 8. PMID: 21640702
Data show that RelB is inducibly phosphorylated by GSK-3beta, indicating a direct substrate-enzyme relationship. PMID: 21217772
epigenetic RELB silencing as a new marker of the progressive disease in males PMID: 21062507
Bovine foamy virus transactivator BTas interacts with cellular RelB to enhance viral transcription. PMID: 20844054
REQ functions as an efficient adaptor protein between the SWI/SNF complex and RelB/p52 and plays important roles in noncanonical NF-kappaB transcriptional activation and its associated oncogenic activity. PMID: 20460684
the Tio oncoprotein triggers noncanonical NF-kappaB signaling through NEMO-dependent up-regulation of p100 precursor and RelB, as well as through NEMO-independent generation of p52 effector PMID: 20353939
the results suggest that RelB was responsible for the LPS-mediated attachment and may play an important role in the progression of some cancers. PMID: 19903458
Rel activity plays role in regulation of apoptosis in hepatocellular carcinoma through activation of downstream target genes PMID: 12365017
During dendritic cell maturation, rapidly activated dimers (e.g., RelA) bound to a subset of target promoters are gradually replaced by slowly activated dimers (e.g., RelB). PMID: 12820969
RelB has an effect on p100 processing, which is possibly regulated in a signal-dependent manner PMID: 12874295
RelB mediates TNF-induced up-regulation of the human polymeric Ig receptor. PMID: 15265917
RelB overexpression promoted, whereas endogenous RelB inhibition (by p100DeltaN) blocked, precursor cell development along this DC subset pathway. PMID: 15315978
induced by cytomegalovirus (CMV) immediate-early 1 protein via activation of JNK and AP-1 PMID: 15596805
RelB expression during dendritic cells differentiation is controlled by protein kinase CbetaII-mediated regulation of transcriptional initiation and elongation PMID: 16107733

Hide All

Involvement in disease

Immunodeficiency 53 (IMD53)

Subcellular Location

Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Colocalizes with NEK6 in the centrosome.

Database Links

HGNC: 9956

OMIM: 604758

KEGG: hsa:5971

STRING: 9606.ENSP00000221452

UniGene: Hs.654402

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：
Please enter the characters shown. Click the image to refresh if unclear.

Code	CSB-RA130517A0HU
Size	US$210
	Order now
Image	Immunofluorescence staining of Hela with CSB-RA130517A0HU at 1:25, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 507-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing Hela cells stained with CSB-RA130517A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1µg/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
Have Questions?	Leave a Message or Start an on-line Chat

RELB Recombinant Monoclonal Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background