Code | CSB-RA253226A0HU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
IHC | 1:50-1:200 |
IF | 1:50-1:200 |
CUSABIO developed the RGS6 recombinant monoclonal antibody through the following steps. First, B cells were isolated from the spleen of an immunized animal, using a synthesized peptide derived from human RGS6 as the immunogen. The isolated B cells underwent RNA extraction, followed by reverse transcription to generate cDNA. Using the cDNA as the template, the gene encoding the RGS6 antibody was then amplified using a degenerate primer and inserted into a vector. Through transfection, the recombinant vector was introduced into host cells, enabling the efficient expression of the RGS6 recombinant monoclonal antibodies. After cell culture, these antibodies were harvested from the supernatant and purified using affinity chromatography. The RGS6 recombinant monoclonal antibody has been demonstrated to detect human RGS6 protein in ELISA, IHC, and IF applications.
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