RGS6 Antibody

Datasheet

Code	CSB-PA019658LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: Rat heart tissue All lanes: RGS6 antibody at 8μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 55, 57, 54, 50, 51, 53, 52, 41, 56 kDa Observed band size: 55 kDa IHC image of CSB-PA019658LA01HU diluted at 1:300 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA019658LA01HU diluted at 1:300 and staining in paraffin-embedded human melanoma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of Hela cells with CSB-PA019658LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) RGS6 Polyclonal antibody

Uniprot No.

P49758

Target Names

RGS6

Alternative Names

FLJ43552 antibody; G protein signaling 6 regulator antibody; GAP antibody; GTPase activating protein antibody; MGC142132 antibody; Regulator of G protein signaling 6 antibody; Regulator of G protein signalling 6 antibody; Regulator of G-protein signaling 6 antibody; RGS 6 antibody; RGS6 antibody; RGS6_HUMAN antibody; S914 antibody

Raised in

Rabbit

Species Reactivity

Human, Rat

Immunogen

Recombinant Human Regulator of G-protein signaling 6 protein (177-262AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The RGS6 Antibody (Product code: CSB-PA019658LA01HU) is Non-conjugated. For RGS6 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA019658LB01HU	RGS6 Antibody, HRP conjugated	ELISA
FITC	CSB-PA019658LC01HU	RGS6 Antibody, FITC conjugated
Biotin	CSB-PA019658LD01HU	RGS6 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:200-1:500
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Regulates G protein-coupled receptor signaling cascades. Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound form. The RGS6/GNB5 dimer enhances GNAO1 GTPase activity.

Gene References into Functions

the results suggest that HA117 regulates the development of drug resistance in CT26 cells in vitro and in vivo. PMID: 28731026
study shows that HA117 potentially promotes the stem-like signature of the HL60/ATRA cell line by inhibiting by the ubiquitination and degradation of DNMT1 and by down-regulating the expression of the GGL domain of RGS6 PMID: 28665981
genetic association studies on a population in Republic of Korea: Data suggest that an SNP in RGS6 (rs2239219) is closely linked to stress-induced abdominal obesity in the population studied. PMID: 28090039
Data support the notion that the Galpha, but not Gbetagamma, arm of the Gi/o signalling is involved in TRPC4 activation and unveil new roles for RGS and RGS6 in fine-tuning TRPC4 activities. PMID: 26987813
Our results suggest that HA117 is a strong multidrug resistance gene. PMID: 20635168
This suggests that HA117 might be an important resistance gene in pediatric solid tumors. PMID: 23619123
No significant association between SNPs of RGS6 and central adiposity has been found. PMID: 26340433
reduced RGS6 expression is associated with poor survival in colorectal cancer patients, suggesting that RGS6 expression may serve as an important prognostic marker PMID: 26653562
these date demonstrate that RGS6 decreases in tumor tissue and may serve as a novel biomarker for outcomes in pancreatic cancer patients and be a potential therapeutic target potential therapeutic target. PMID: 25120791
we found a novel mutation in RGS6, the splice-acceptor variant c.1369-1G>C that was not previously reported in congenital cataract phenotypes. PMID: 25525169
genetic association studies in Hispanic-American families in Texas and Colorado: SNP in RGS6 are associated with high dietary fat intake, food preferences, and adiposity/obesity phenotype PMID: 21233807
RGS6-induced apoptosis in both breast cancer cells and mouse embryonic fibroblasts does not require its GAP activity toward G proteins PMID: 21041304
interacts with SCG10 and promotes neuronal differentiation; role of the G gamma subunit-like (GGL) domain of this protein PMID: 12140291
Role of the RGS domain in stress-induced trafficking of RGS6 protein PMID: 12761220
RGS6 gene structure, complex alternative splicing, and role of N terminus and G protein gamma-subunit-like (GGL) domain in subcellular localization of RGS6 splice variants PMID: 12761221
RGS6 is the first member of the RGS protein family shown to interact with proteins involved in transcriptional regulation. PMID: 14734556

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Subcellular Location

Cytoplasm. Cytoplasm, cytosol. Membrane; Peripheral membrane protein. Nucleus. Cell membrane.

Database Links

HGNC: 10002

OMIM: 603894

KEGG: hsa:9628

STRING: 9606.ENSP00000451030

UniGene: Hs.509872

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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