RHOB&RHOA&RHOC Recombinant Monoclonal Antibody

The Rho GTPases RhoA, RhoB, and RhoC constitute a closely related subfamily of small signaling proteins that serve as molecular switches regulating actin cytoskeleton dynamics, cell motility, and cytokinesis. While sharing significant sequence homology, each family member exhibits distinct subcellular localization patterns and biological functions. RhoA and RhoC are primarily associated with stress fiber formation and cell migration, with RhoC frequently implicated in metastatic progression, whereas RhoB localizes to endosomes and functions in receptor trafficking and apoptotic signaling. The overlapping yet distinct roles of these proteins make them compelling targets in cancer biology, developmental studies, and investigations of cytoskeletal regulation.

This recombinant rabbit monoclonal antibody, clone 23H9, recognizes all three Rho family members, providing researchers with a versatile tool for studying total Rho subfamily expression. The recombinant production method ensures consistent performance across experiments, with defined sequence identity eliminating the lot-to-lot variability that can complicate longitudinal studies. Affinity chromatography purification delivers a reagent suitable for quantitative applications where reproducibility is paramount.

Validation studies confirm robust performance in Western blot analysis, where the antibody detects a band at the expected 22 kDa molecular weight in SH-SY5Y neuroblastoma cell lysates at dilutions between 1:500 and 1:2000. Flow cytometry applications have been validated using Jurkat T cells with intracellular staining protocols, demonstrating clear separation from isotype control at 1:50 to 1:200 dilutions.

This antibody supports research into cytoskeletal regulation, cancer cell invasion mechanisms, and signal transduction pathways where Rho GTPase activity drives cellular phenotypes.