Code | CSB-RA940350A0HU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
WB | 1:500-1:2000 |
FC | 1:50-1:200 |
The production of the RPS19 recombinant monoclonal antibody follows a stringent and well-defined process to ensure its quality and specificity. Initially, B cells are isolated from an immunized animal using the synthesized peptide derived from human RPS19 as the immunogen. Total RNA is extracted from the harvested B cells and converted into cDNA through reverse transcription. The RPS19 antibody genes are then amplified using specific primers designed for the antibody constant regions and inserted into an expression vector. This vector is introduced into host cells to facilitate the production of the RPS19 recombinant monoclonal antibody. The antibody is harvested from the cell culture supernatant and undergoes purification using affinity chromatography, resulting in a highly purified form. To validate its specificity and functionality, the antibody undergoes comprehensive characterization assays, including ELISA, WB, and FC analysis, ensuring its accurate recognition of human RPS19 protein.
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