RPS20 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA215735A0HU
Size	US$210
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Image	Western Blot , Positive WB detected in:Hela whole cell lysate(30µg), HepG2 whole cell lysate(30µg), COLO205 whole cell lysate(30µg), THP-1 whole cell lysate(30µg), K562 whole cell lysate(30µg), Jurkat whole cell lysate(30µg), MCF7 whole cell lysate(30µg), Mouse brainr tissue lysate(30µg), Rat brain lysate(30µg) All lanes: RPS20 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 13 kDa Observed band size: 13 kDa Exposure time：60s IHC image of CSB-RA215735A0HU diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA215735A0HU diluted at 1:100 and staining in paraffin-embedded human colorectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Overlay Peak curve showing jurkat cells stained with CSB-RA215735A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Target Background

Product Details

Uniprot No.

P60866

Target Names

RPS20

Alternative Names

40S ribosomal protein S20 antibody; Ribosomal protein S20 antibody; RPS 20 antibody; RpS20 antibody; RS20_HUMAN antibody; S20 antibody

Species Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide from human RPS20 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

24B4

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Gene References into Functions

We therefore found published evidence to support the association between variants in NTHL1 and RPS20 with CRC. PMID: 27713038
Mutations in RPS20 can predispose individuals to microsatellite-stable hereditary nonpolyposis colorectal carcinoma. PMID: 24941021
Gain of 8q-mapped RPS20 is associated with adverse outcome in Medulloblastoma. PMID: 16968546

Subcellular Location

Cytoplasm.

Protein Families

Universal ribosomal protein uS10 family

Database Links

HGNC: 10405

OMIM: 603682

KEGG: hsa:6224

STRING: 9606.ENSP00000429374

UniGene: Hs.8102

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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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