RPS20 Recombinant Monoclonal Antibody

RPS20 is a core component of the 40S ribosomal subunit, playing an essential role in protein synthesis and ribosome biogenesis. As a fundamental element of the cellular translation machinery, RPS20 has garnered significant research interest in studies examining ribosomal function, protein production dynamics, and the growing field of ribosomopathies—conditions linked to ribosomal protein dysfunction that can manifest in developmental disorders and cancer predisposition.

This recombinant monoclonal antibody, generated from clone 24B4, offers the reproducibility and consistency that demanding research workflows require. Because recombinant antibodies are produced from defined sequences rather than traditional hybridoma methods, you can expect reliable performance across experiments and between lots, eliminating a common source of variability in long-term studies.

Validation testing demonstrates robust performance across multiple applications. Western blot analysis confirms specific detection at the expected 13 kDa molecular weight across an extensive panel of human cell lines including HeLa, HepG2, COLO205, THP-1, K562, Jurkat, and MCF7, with cross-species reactivity confirmed in mouse and rat brain tissue lysates. For tissue-based studies, immunohistochemistry has been validated in paraffin-embedded human brain and colorectal cancer sections using citrate buffer antigen retrieval. Flow cytometry applications have been demonstrated in Jurkat cells with clear separation from isotype controls, enabling quantitative analysis of RPS20 expression at the single-cell level.

This antibody serves researchers investigating ribosome biology, translational regulation, and cancer metabolism, where ribosomal protein expression patterns may serve as markers of cellular proliferation or stress responses.