RPS8 Recombinant Monoclonal Antibody

RPS8, or 40S ribosomal protein S8, serves as an essential structural component of the small ribosomal subunit, playing a fundamental role in protein synthesis across all cell types. As a core element of the translational machinery, RPS8 has garnered significant research interest in studies examining ribosome biogenesis, translational regulation, and the emerging connections between ribosomal proteins and cellular proliferation, particularly in cancer biology.

This recombinant monoclonal antibody, generated in rabbit against a synthetic peptide derived from human RPS8, offers the reproducibility and consistency that demanding experimental workflows require. Because the antibody sequence is defined and production occurs through recombinant expression, researchers can expect uniform performance across lots, eliminating the variability often encountered with traditional hybridoma-derived antibodies.

Extensive validation across multiple platforms demonstrates this antibody's versatility in your experimental toolkit. Western blot analysis confirms reliable detection across a diverse panel of human cell lines, including HeLa, HepG2, A431, U-251MG, K562, SH-SY5Y, Jurkat, THP-1, and HEK293 lysates. The observed band at approximately 30 kDa, slightly higher than the predicted 24 kDa molecular weight, likely reflects post-translational modifications such as glycosylation or phosphorylation events common to ribosomal proteins. Immunohistochemistry validation in human placenta tissue demonstrates clear staining with appropriate antigen retrieval, while immunofluorescence studies in HeLa cells reveal the expected cytoplasmic localization pattern. Flow cytometry analysis using Jurkat cells further confirms the antibody's utility for single-cell applications.

Whether investigating ribosomal function, translational control mechanisms, or exploring RPS8's potential extra-ribosomal roles in disease contexts, this antibody provides a dependable research tool with demonstrated performance across complementary detection methods.