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S100A6 Recombinant Monoclonal Antibody

Datasheet
Code CSB-RA441420A0HU
Size US$210
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Image
  • Western Blot
    Positive WB detected in: U-251MG whole cell lysate(30µg), A549 whole cell lysate, HeLa whole cell lysate(30µg), SW620 whole cell lysate(30µg), SH-SY5Y whole cell lysate(30µg)
    All lanes: S100A6 antibody at 1:1000
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 10 kDa
    Observed band size: 10 kDa
    Exposure time: 1min
  • Overlay Peak curve showing MCF-7 cells stained with CSB-RA441420A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Product Details

Uniprot No.
P06703
Target Names
S100A6
Alternative Names
2A9 antibody; 5B10 antibody; CABP antibody; CACY antibody; Calcyclin antibody; Growth factor inducible protein 2A9 antibody; Growth factor-inducible protein 2A9 antibody; MLN 4 antibody; MLN4 antibody; OTTHUMP00000015472 antibody; OTTHUMP00000015473 antibody; PRA antibody; PRAGrowth factor inducible protein 2A9 antibody; Prolactin receptor associated protein antibody; Prolactin receptor-associated protein antibody; Protein S100 A6 antibody; Protein S100-A6 antibody; S100 A6 antibody; S100 calcium binding protein A6 (calcyclin) antibody; S100 calcium binding protein A6 antibody; S100 calcium-binding protein A6 antibody; S100A6 antibody; S10A6_HUMAN antibody
Species Reactivity
Human
Immunogen
A synthesized peptide derived from human S100A6
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone No.
10F2
Purification Method
Affinity-chromatography
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Form
Liquid
Tested Applications
ELISA, WB, FC
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:2000
FC 1:50-1:200
Protocols
ELISA Protocol
Western Blotting(WB) Protocol
Flow Cytometry (FC) Protocol
Troubleshooting and FAQs
Antibody FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

S100A6, also known as calcyclin, is a calcium-binding protein belonging to the S100 family that plays important roles in cell cycle regulation, cytoskeletal dynamics, and cellular stress responses. This protein is notably upregulated in various cancers and has been implicated in tumor progression, making it a valuable target for researchers investigating proliferative signaling pathways, calcium-dependent cellular processes, and oncogenic mechanisms.

This recombinant monoclonal antibody, clone 10F2, offers the reproducibility and consistency that demanding research applications require. Generated against a synthetic peptide derived from human S100A6 and produced using recombinant technology in rabbit host, this antibody provides sequence-defined specificity with minimal lot-to-lot variation. The affinity-chromatography purification ensures high purity, supporting reliable and reproducible results across your experimental timeline.

Validation studies demonstrate robust performance across multiple platforms. In western blot applications, this antibody detects a clean band at the predicted 10 kDa molecular weight across diverse human cell lines including U-251MG glioblastoma, A549 lung carcinoma, HeLa cervical cancer, SW620 colorectal adenocarcinoma, and SH-SY5Y neuroblastoma cells, with recommended dilutions ranging from 1:500 to 1:2000. Flow cytometry validation in MCF-7 breast cancer cells confirms intracellular detection capability with clear separation from isotype control, supporting quantitative analysis of S100A6 expression at the single-cell level. The antibody is also validated for ELISA applications.

Whether you are investigating calcium signaling networks, characterizing tumor cell phenotypes, or exploring S100A6 as a potential biomarker, this recombinant antibody delivers the experimental flexibility and technical reliability needed for meaningful research outcomes.

Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Customer Reviews and Q&A

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Target Background

Function
May function as calcium sensor and modulator, contributing to cellular calcium signaling. May function by interacting with other proteins, such as TPR-containing proteins, and indirectly play a role in many physiological processes such as the reorganization of the actin cytoskeleton and in cell motility. Binds 2 calcium ions. Calcium binding is cooperative.
Gene References into Functions
  1. aberrant S100A16 expression might be modulated by its DNA hypomethylation and serves as an independent prognostic indicator of unfavorable overall survival and recurrence-free survival in non-small cell lung adenocarcinoma PMID: 29746588
  2. High expression of S100A6 in ICC defines a special group of patients with worse clinical characteristics and outcomes. Besides, S100A6 may promote ICC proliferation via activating p38/MAPK pathway. PMID: 28984474
  3. The results suggest that binding of S100A6 to integrin beta1 affects cell adhesion/proliferation due to activation of ILK and FAK signaling pathways. PMID: 29020611
  4. Authors demonstrated that the expression of S100A6 was significantly associated with patient age and tumor differentiation. PMID: 29053662
  5. Data show that S100 calcium binding protein A6 (S100A6) is required for the Ca2+-dependent nuclear translocation of calcyclin binding protein (CacyBP/SIP) in colon cancer SW480 cells. PMID: 29534068
  6. Isothermal titration calorimetry measured the binding of peptides with diverse sequence and biochemistry to human S100A5 and S110A6. These proteins bound distinct, but overlapping, sets of peptide targets. The specificity of S100 peptide interfaces is likely important for the biology of these proteins. PMID: 29240404
  7. S100A6 is a potential risk marker for screening of cholangiocarcinoma. PMID: 29629840
  8. The S100A6 interacts with FOR20 and related centrosomal proteins through a conserved N-terminal domain, suggesting a novel Ca(2+)-dependent regulation of centrosomal function. PMID: 28765046
  9. REVIEW: summary of novel discoveries concerning S100A6 targets, its involvement in cellular signaling pathways, and presence in stem/progenitor cells, extracellular matrix and body fluids of diseased patients PMID: 28343163
  10. Results showed that S100A6 was markedly up-regulated in nasopharyngeal carcinoma (NPC) tissues and cell lines and, may promote NPC development via the activation of p38/MAPK signaling pathways. PMID: 27596819
  11. As an intracellular protein, S100A6 has been implicated in the regulation of several cellular functions, such as proliferation, apoptosis, the cytoskeleton dynamics, and the cellular response to different stress factors. S100A6 can be secreted/released by certain cell types which points to extracellular effects of the protein. [review] PMID: 28417162
  12. suppression of S100A6 expression with RNAi significantly decreased the expression levels of beta-catenin, inhibited the growth and migration of eutopic endometrial stromal cells, and promoted their apoptosis. PMID: 28075439
  13. Study reports the crystallographic structure of an S100-bound full-length RAGE ectodomain. The structure reveals a unique dimeric conformation of RAGE, which appears suited for signal transduction, and shows that the S100A6 protein adopts a non-canonical homodimeric arrangement. PMID: 27818100
  14. STAT1 suppression by S100A6 may represent a promising therapeutic target to facilitate reendothelialization in damaged vessels. PMID: 27386938
  15. findings strongly suggest that S100A6 may promote OS cell proliferation and OS tumor growth at least in part by facilitating cell cycle progression, preventing apoptosis, and inhibiting osteogenic differentiation PMID: 26646427
  16. S100A6 induces EMT and promotes cell migration and invasion in a beta-catenin-dependent manner. PMID: 25799022
  17. Weak or absent S100A6 staining supports a diagnosis of pilar leiomyoma, whereas strong positive staining supports a diagnosis of cutaneous leiomyosarcoma. PMID: 26238340
  18. Elevated S100A6 enhances tumorigenesis and suppresses CXCL14-induced apoptosis in clear cell renal cell carcinoma. PMID: 25760073
  19. Applying this serum marker to clinical practice would require less-invasive examinations of patients and would help to detect life-threatening cancerous lesions earlier than current modalities. PMID: 25743341
  20. Results obtained proved that increased S100A6 content in keratinocytes dramatically changed the pace and extent of epidermal differentiation PMID: 25450463
  21. High-level S100A6 promotes metastasis and predicts the outcome of T1-T2 stage in clear cell renal cell carcinoma PMID: 25120023
  22. Data indicate that the interaction between S100A6 and integrin beta1, was confirmed by ELISA. PMID: 25256682
  23. In Wharton's jelly of preeclamptic tissue S100 calcium binding protein A6(S100A6) is up-regulated and binds to different targets than in control suggesting involvement in development of preeclampsia PMID: 24746261
  24. Following myocardial infarction, S100A6 expression levels increased in cardiomyocytes. PMID: 23844739
  25. S100A6 may be involved in promotion and progression of human liver cancer. PMID: 24281831
  26. Expression of S100A6 and MMP9 in combination is associated with the development of squamous cell carcinoma. PMID: 23993025
  27. S100A6 and the TAZ2 domain of p300 bind p53 with similar affinities and S100A6 effectively competes with TAZ2 for binding to p53. PMID: 23796514
  28. The S100A6 mutant protein interacts with the RAGE V domain in a symmetrical fashion forming a heterotetrameric complex. PMID: 23537648
  29. native S1006 seeds SOD1 aggregation, shortening its nucleation process. This suggests a cross-talk between these two proteins involved in ALS. PMID: 23076148
  30. Results show strong correlations between mRNA levels of PRA and PRB, protein levels of hormone receptors, HER/ErbB receptors and ligands network, and suggest that crosstalks between PR and the HER family are a hallmark of breast cancer growth. PMID: 22505232
  31. Depletion of endothelial S100A6 levels also elevated beta-galactosidase expression, which is an important hallmark of cellular senescence and exit from the mammalian cell cycle PMID: 23095053
  32. Upregulated expression of S100A6 is associated with gastric cancer. PMID: 22681645
  33. Introduced a simple and efficient method for producing high-purity recombinant human S100A6 from Escherichia coli culture with low level of endotoxin. We further demonstrated its biological activities for triggering SH-SY5Y cells apoptosis in vitro. PMID: 22450162
  34. S100A6 interacts with lamin A/C, a protein known to be implicated in colon carcinogenesis. PMID: 22560296
  35. the overexpression of thioredoxin,S100-A10 and S100-A6 specifically distinguished metastatic from non-metastatic tumors PMID: 21938494
  36. new insight into the interaction between S100 proteins and CacyBP/SIP PMID: 22295074
  37. Calcyclin was identified to be underexpressed in small cell lung cancers, as compared with non-small cell lung cancers and normal lung tissue. PMID: 22192801
  38. results show the presence of S100A6 in umbilical cord and suggest the involvement of this protein in intra- and extra-cellular signalling pathways in this tissue PMID: 21923636
  39. S100A6 is necessary for nuclear translocation of the Sgt1 protein PMID: 20213445
  40. Serum levels of S100B, S100A6 and S100P are associated with acute coronary syndrome, and serum levels and myocardial expression of these proteins are related to infarct size. PMID: 21663912
  41. In melanocytic neoplasms composed of small spindle cells, patchy S100A6 staining should not be interpreted as evidence of supporting a diagnosis of melanoma PMID: 21039744
  42. Results suggested that S100A6 plays an important role in the progression of gastric cancer, affecting patient prognosis, and is up-regulated by epigenetic regulation. PMID: 20581057
  43. This study supports a role of S100A6 in thyroid tumorigenesis and as a potential aid in the discrimination between follicular thyroid tumors and papillary thyroid carcinoma. PMID: 20629554
  44. S100A6 expression decreases along with cancerization in oral squamous cell carcinoma. PMID: 20596636
  45. S100A6 expression level in cancer and metastatic lymph node was significantly higher than their matched non-neoplastic mucosa. S100A6 overexpression was associated with larger tumor size and deeper invasion. PMID: 19062716
  46. S100A6 concentration predicts peritoneal tumor burden in mice with epithelial ovarian cancer and is associated with advanced stage in patients PMID: 19888321
  47. Proteomic profiling in distinct cellular compartments led to the identification of a novel p53-dependent biomarker of telomere dysfunction, S100A6. PMID: 19834903
  48. findings suggest that up-regulation of FRalpha gene and calcyclin gene expressions induced by Allitridi may play an important role in human gastric cancer cell differentiation PMID: 11925593
  49. Results are consistent with S100A6, and most likely other S100 proteins, functioning as Ca(2+) sensors in a way analogous to the prototypical sensors calmodulin and troponin C. PMID: 11937060
  50. Sgt1 binds to S100A6 in a calcium-regulated manner PMID: 12746458

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Subcellular Location
Nucleus envelope. Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side.
Protein Families
S-100 family
Database Links

HGNC: 10496

OMIM: 114110

KEGG: hsa:6277

STRING: 9606.ENSP00000357708

UniGene: Hs.275243

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