SAFB Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA018252A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: COLO-205 whole cell lysate(30µg), A-431 whole cell lysate(30µg), HEK293 whole cell lysate(30µg), THP-1 whole cell lysate(30µg), Mouse brain tissue lysate(30µg), Rat brain tissue lysate(30µg) All lanes: SAF B antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 103 kDa Observed band size: 150 kDa Exposure time：1min IHC image of CSB-RA018252A0HU diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA018252A0HU diluted at 1:100 and staining in paraffin-embedded human colorectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of HeLa cell with CSB-RA018252A0HU at 1:50 , counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing jurkat cells stained with CSB-RA018252A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Product Details
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Target Background

Product Details

Uniprot No.

Q15424

Target Names

SAFB

Alternative Names

DKFZp779C1727; glutathione S transferase fusion protein; HAP; HET; HSP27 ERE TATA binding protein; HSP27 ERE-TATA-binding protein; HSP27 estrogen response element-TATA box-binding protein; SAF B; SAF-B; SAF-B1; SAFB 1; SAFB; SAFB1; SAFB1_HUMAN; Scaffold attachment factor B1

Species Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide from human SAFB protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

5G1

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Scaffold attachment factor B1 (SAFB) serves as a multifunctional nuclear protein that plays essential roles in chromatin organization, transcriptional regulation, and RNA processing. Originally identified through its interaction with scaffold/matrix attachment region DNA elements, SAFB has emerged as a significant player in estrogen receptor signaling pathways and stress response mechanisms, making it a valuable target for researchers investigating gene regulation, hormone-dependent cancers, and cellular stress biology.

This recombinant monoclonal antibody, generated against a synthetic peptide derived from human SAFB, offers the reproducibility and consistency that demanding experimental workflows require. As a sequence-defined reagent produced through recombinant technology, it eliminates the lot-to-lot variability that can compromise longitudinal studies, ensuring your results remain comparable across experiments and publications.

Extensive validation across multiple platforms demonstrates this antibody's versatility in your research toolkit. Western blot analysis confirms robust detection in human cell lines including COLO-205, A-431, HEK293, and THP-1, with cross-species reactivity verified in mouse and rat brain tissue lysates. The observed band at approximately 150 kDa, higher than the predicted 103 kDa molecular weight, likely reflects post-translational modifications such as glycosylation or phosphorylation characteristic of this heavily modified nuclear protein. Immunohistochemical staining has been validated in human small intestine and colorectal cancer tissues, while immunofluorescence studies in HeLa cells and flow cytometry analysis of Jurkat cells further expand your experimental options.

Whether investigating SAFB's role in transcriptional regulation, exploring its function in cancer biology, or examining stress response pathways, this antibody provides reliable detection across diverse experimental contexts and model systems.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Binds to scaffold/matrix attachment region (S/MAR) DNA and forms a molecular assembly point to allow the formation of a 'transcriptosomal' complex (consisting of SR proteins and RNA polymerase II) coupling transcription and RNA processing. Functions as an estrogen receptor corepressor and can also bind to the HSP27 promoter and decrease its transcription. Thereby acts as a negative regulator of cell proliferation. When associated with RBMX, binds to and stimulates transcription from the SREBF1 promoter.

Gene References into Functions

SAFB regulated the activity of NF-kappaB signaling in CRC by targeting TAK1 This novel mechanism provides a comprehensive understanding of both SAFB and the NF-kappaB signaling pathway in the progression of CRC and indicates that the SAFB-TAK1-NF-kappaB axis is a potential target for early therapeutic intervention in CRC progression PMID: 28912140
Depletion of SAFB1 reduced FUS's localization to chromatin-bound fraction and splicing activity, suggesting SAFB1 could tether FUS to chromatin compartment thorough N-terminal DNA-binding motif. Moreover, FUS interacts with another nuclear matrix-associated protein, Matrin3. PMID: 27731383
Data suggest that ERH interacts directly in nucleus with C-terminal Arg-Gly-rich region of SAFB1/SAFB2 and this multimer co-localizes in insoluble nuclear fraction; binding of ERH reverses inhibition exerted by SAFB1/SAFB2 on SRPK1. (ERH = enhancer of rudimentary homolog protein; SAFB = scaffold attachment factor B; SRPK1 = splicing kinase SR protein kinase-1) PMID: 28627136
The expression of coding and non-coding genes with SAFB1 cross-link sites was altered by SAFB1 knockdown. The isoform-specific expression of neural cell adhesion molecule (NCAM1) and ASTN2 was influenced by SAFB1. PMID: 26694817
Single depletion of either SAFB1 or SAFB2 leads to an increase in expression of the other SAFB protein. PMID: 26273616
reveals an unexpected role of SUMO-1 and SAFB in the stimulatory coupling of promoter binding, transcription initiation and RNA processing PMID: 25800734
SAFB1 formed a complex with the histone methyltransferase EZH2. PMID: 23893242
Data indicate that scaffold attachment factor SAFB1 is transiently recruited to DNA breaks in a poly(ADP-ribose)-polymerase 1- and poly(ADP-ribose)-dependent manner. PMID: 24055346
Results indicate that SAFB1 and SAFB2 are crucial repressors for ERalpha dynamics in association with the nuclear matrix and that their synergistic regulation of ERalpha mobility is sufficient for inhibiting ERalpha function. PMID: 22566185
transcriptional repressor SAFB1 is modified by both SUMO1 and SUMO2/3, and this modification is necessary for its full repressive activity. PMID: 21527249
Data show that binding of p53 to SAFB1 had a significant functional outcome, since SAFB1 was shown to suppress p53-mediated reporter gene expression. PMID: 21130767
This study shows that low SAFB protein levels predict poor prognosis of breast cancer patients, suggesting critical functions of SAFB1 and SAFB2 in breast cancer cells. PMID: 19137425
Study confirms the primary role of SAFB1/SAFB2 as corepressors and also uncovers a previously unknown role for SAFB1 in the regulation of immune genes and in estrogen-mediated repression of genes. PMID: 19901029
HSP27 is a nuclear speckle component in unstressed cells in tissue culture. It is also associated with the nucleolar compartment. PMID: 12837281
REVIEW: possibility that SAFB1 and SAFB2 are novel breast tumor suppressor genes, and how they might function in this role, are discussed PMID: 14587024
HSP27 expression may have useful diagnostic use for the prognosis of mouth squamous cell carcinoma. PMID: 14702179
SAFB1 represses ERalpha activity via indirect association with histone deacetylation and interaction with the basal transcription machinery PMID: 15066997
SAFB1 was shown to interact directly with the nuclear receptor corepressor N-CoR. PMID: 16195251
SAFB1 interacts in pull-down assays not only with PPARgamma but also with all nuclear receptors tested PMID: 16326836
SAFB may direct the reorganization and segregation of nuclear RNA and DNA prior to endonuclease-mediated DNA cleavage. PMID: 17643427
Over expression of HSP27 is associated with intrahepatic cholangiocarcinoma PMID: 18154639
hXOR is a tumor suppressor-targeted gene and the phosphorylation of SAFB1 is regulated by OSM, which provides a molecular basis for understanding the role of SAFB1-regulated hXOR transcription in cytokine stimulation and tumorigenesis PMID: 18772145
SAFB1 is not likely to be causative of the hereditary breast cancer syndrome in west Swedish breast cancer families PMID: 19077293
Importance of ERE-BP as an attenuator of normal ERalpha signaling in vivo and is a novel target for modulation by selective estrogen receptor modulators. PMID: 19106221
The enzymatic activity of SR protein kinases 1 and 1a is negatively affected by interaction with scaffold attachment factors B1 and 2. PMID: 19674106

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Subcellular Location

Nucleus.

Tissue Specificity

Ubiquitous. Expressed at high levels in the CNS and at low levels in the liver. Expressed in a wide number of breast cancer cell lines.

Database Links

HGNC: 10520

OMIM: 602895

KEGG: hsa:6294

STRING: 9606.ENSP00000292123

UniGene: Hs.728802

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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