Product Details

Uniprot No.

Target Names

SATB1

Alternative Names

DNA binding protein SATB1 antibody; DNA-binding protein SATB1 antibody; SATB homeobox 1 antibody; SATB1 antibody; SATB1_HUMAN antibody; Special AT rich sequence binding protein 1 (binds to nuclear matrix/scaffold associating DNA) antibody; Special AT rich sequence binding protein 1 antibody; Special AT-rich sequence-binding protein 1 antibody

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human SATB1

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

7A9

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

SATB1 (Special AT-rich sequence-binding protein 1) serves as a critical chromatin organizer and transcription factor that regulates gene expression by anchoring DNA to the nuclear matrix. This protein plays essential roles in T-cell development, immune function, and has garnered significant attention in cancer research due to its involvement in tumor progression and metastasis. Understanding SATB1 expression and function is fundamental for researchers investigating chromatin architecture, immune cell differentiation, and oncogenic mechanisms.

This recombinant monoclonal antibody, clone 7A9, offers the consistency and reliability that demanding experimental workflows require. Produced using recombinant technology with a defined sequence, it eliminates the lot-to-lot variability that can compromise longitudinal studies and reproducibility. The rabbit host-derived IgG format, combined with affinity chromatography purification, ensures high specificity for your human SATB1 detection needs.

Validation in Western blot applications demonstrates robust performance in relevant immune cell models, with clear detection in both THP-1 monocytic and Jurkat T-cell lysates at dilutions ranging from 1:500 to 1:2000. The observed band at approximately 100 kDa, slightly higher than the predicted 86 kDa molecular weight, likely reflects post-translational modifications such as phosphorylation or sumoylation, which are known to regulate SATB1 activity in vivo. This antibody is also validated for ELISA applications, providing flexibility across different experimental platforms.

Whether you are investigating T-cell biology, exploring chromatin organization mechanisms, or examining SATB1's role in cancer progression, this antibody delivers the specificity and reproducibility needed to advance your research with confidence.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Crucial silencing factor contributing to the initiation of X inactivation mediated by Xist RNA that occurs during embryogenesis and in lymphoma. Binds to DNA at special AT-rich sequences, the consensus SATB1-binding sequence (CSBS), at nuclear matrix- or scaffold-associated regions. Thought to recognize the sugar-phosphate structure of double-stranded DNA. Transcriptional repressor controlling nuclear and viral gene expression in a phosphorylated and acetylated status-dependent manner, by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin-loop remodeling. Acts as a docking site for several chromatin remodeling enzymes (e.g. PML at the MHC-I locus) and also by recruiting corepressors (HDACs) or coactivators (HATs) directly to promoters and enhancers. Modulates genes that are essential in the maturation of the immune T-cell CD8SP from thymocytes. Required for the switching of fetal globin species, and beta- and gamma-globin genes regulation during erythroid differentiation. Plays a role in chromatin organization and nuclear architecture during apoptosis. Interacts with the unique region (UR) of cytomegalovirus (CMV). Alu-like motifs and SATB1-binding sites provide a unique chromatin context which seems preferentially targeted by the HIV-1 integration machinery. Moreover, HIV-1 Tat may overcome SATB1-mediated repression of IL2 and IL2RA (interleukin) in T-cells by binding to the same domain than HDAC1. Delineates specific epigenetic modifications at target gene loci, directly up-regulating metastasis-associated genes while down-regulating tumor-suppressor genes. Reprograms chromatin organization and the transcription profiles of breast tumors to promote growth and metastasis. Promotes neuronal differentiation of neural stem/progenitor cells in the adult subventricular zone, possibly by positively regulating the expression of NEUROD1.

Gene References into Functions

SATB1 may reprogram energy metabolism in ovarian cancer by regulating lactate dehydrogenase and MCT1 levels to promote metastasis. PMID: 30132561
Studies indicate Special AT-rich Sequence Binding Protein 1 (SATB1) to affect proliferation, cell cycle, apoptosis, cell morphology / cell polarity, EMT and multidrug-resistance as well as tumor formation, growth, invasion and metastasis [Review]. PMID: 29306014
Study results in bladder cancer are consistent with the conflicting data reported in other cancers, and that SATB1 might have different roles in cancer dependent on genetic background and stage of the cancer. The role of SATB1 is dependent on multiple factors, its use as a biomarker limited and as a therapeutic target likely highly variable among patients. PMID: 29079132
The aim of this study was to investigate the expression of p16 and SATB1 proteins in regard to expression of the Ki-67 antigen and available clinicopathological data (i.a. receptor status, staging and grading PMID: 29936452
HBx upregulated hepatic SATB1. PMID: 27883059
Invasion and metastasis of GIN in Chinese patients correlates with SATB1 overexpression in tumor tissues, most profoundly in gastric cancer. PMID: 28636989
High SATB1 expression is associated with Immunosuppressive Function of Regulatory T Cells in Chronic Hepatitis B. PMID: 28801365
SATB2 can induce dedifferentiation by inducing stemness and may have a role in pancreatic carcinogenesis PMID: 27472393
High SATB1 expression was associated with a better overall survival of patients with non-small cell lung carcinoma.ATB1 level correlates with Ki-67 expression in non-small cell lung carcinoma. PMID: 29374696
These data implied that SATB1 might regulate gene expression through its different oligomerization state. PMID: 28205095
High SATB1 expression is associated with glioblastoma. PMID: 28049521
These findings suggest SATB1 may play an important role in OSCC invasiveness and metastasis. PMID: 27783844
This paper provides evidence that SATB1 plays an oncogenic role in esophageal cancer by up-regulation of FN1 and PDGFRB. PMID: 28147311
combination of the sequence-specific and nonspecific DNA-binding modes of SATB1 should be advantageous in a search for target loci during transcriptional regulation PMID: 27462121
revealed more than 170 NFAT-associated proteins, half of which are involved in transcriptional regulation. Among them are many hitherto unknown interaction partners of NFATc1 and NFATc2 in T cells, such as Raptor, CHEK1, CREB1, RUNX1, SATB1, Ikaros, and Helios. PMID: 27637333
SATB1 interacts directly with OGG1, increases its binding to 8-oxoG-containing DNA, promotes Schiff base formation, and stimulates its glycosylase and apyrimidinic/apurinic lyase enzymatic activities. PMID: 27590341
High SATB1 expression in was detected in 48.3% of esophageal squamous cell carcinoma and 7.8% of normal esophagus tissues. PMID: 25951709
Silencing of special AT-rich sequence binding protein 1 inhibited the proliferation of KYSE450 and EC9706 cells which have a relatively high level of special AT-rich sequence binding protein 1, and the ability of migration and invasion of KYSE450 and EC9706 cells was distinctly suppressed. PMID: 28345457
Results suggest that SATB1 is activated to bind to chromatin at S/MARs after exposure to Abeta 1-42, resulting in alternative utilization and movement of 82-kDa ChAT to these regions demonstrating that both proteins play critical roles in the response of neural cells to acute Abeta-exposure. PMID: 27052102
Our results highlight the significance of SATB1 in intrahepatic cholangiocarcinoma PMID: 26563145
SATB1 expression was decreased in ATL cells compared with normal controls. Knockdown of SATB1 expression significantly enhanced invasion of Jurkat cell in vitro. SATB1 knockdown enhanced beta-catenin nuclear accumulation and transcriptional activity and thus may increase the invasiveness of Jurkat cells through the activation of Wnt/beta-catenin signaling pathway in vitro. PMID: 26678884
SATB1 binds to the upstream region of UCA1 that its promoter activity increases with SATB1 depletion and SATB1 repressed the expression of oncogenic UCA1 suppressing growth and survival. PMID: 27697109
Decreased expression of SATB1 in ccRCC may result from over-expressed miR-21-5p. Our data suggest that SATB1 may have a potential value as a prognostic marker in ccRCC. PMID: 27107063
overexpression of SATB1 was an independent poor prognostic factor in stage I-III, especially in stage II colorectal cancer (CRC). SATB1 may play an important role in LNM of CRC. SATB1 may be a biomarker of LNM and of recurrence after surgery for CRC PMID: 27466515
Results suggested that high expression of SATB1 is significantly correlated with poor differentiation of CRC. SATB1 might promote the epithelial-mesenchymal transition by increasing the aberrant expression of beta-catenin. PMID: 26810818
Logistic regression revealed that four variants in NR3C1 and SATB1 were significantly associated with lung cancer risk after false discovery rate (FDR) correction [For NR3C1, rs9324921: odds ratio (OR)=1.23, P for FDR=0.029; rs12521436: OR=0.85, P for FDR=0.040; rs4912913: OR=1.17, P for FDR=0.040; For SATB1, rs6808523: OR=1.33, P for FDR=0.040]. PMID: 27179949
modulation of epithelial-mesenchymal transition by SATB1 may contribute to prostate cancer metastasis. PMID: 27044805
Tumor-driven, unremitting expression of Satb1 in activated Zbtb46+ inflammatory dendritic cells that infiltrate ovarian tumors results in an immunosuppressive phenotype characterized by increased secretion of tumor-promoting Galectin-1 and IL-6. PMID: 26876172
High expression of SATB1 is associated with colorectal cancer. PMID: 26701851
radiation decreases SATB1 expression and sensitizes cancer cells to confer clinical benefit of patients, suggesting that SATB1 is predictive of response to preoperative RT and clinical outcome in rectal cancer. PMID: 26528635
Findings indicate that high Ki67 antigen/ SATB1 protein ratio was an independent predictor of worse overall survival (OS), while Ki67 and SATB1 did not reach statistical significance as single predictors. PMID: 26512778
SATB1 reprograms the expression of tumor growth- and metastasis-associated genes to promote tumorigenesis and functionally overlaps with Wnt signaling critical for colorectal cancer tumorigenesis. PMID: 26165840
SATB1 as a Dual Regulator of Anti-Apoptotic BCL2 and Pro-Apoptotic NOXA Genes PMID: 26422397
SATB1 protein expression showed an increasing trend in advancing stages of breast cancer development. PMID: 25966097
replicative oncolytic adenovirus armed with SATB1 shRNA exhibits effective antitumor effect in human prostate cancer. Our study provides the basis for the development of ZD55-SATB1 for the treatment of prostate cancer PMID: 26084613
Immunohistochemical expression of SATB1 and SATB2 was analysed in tissue microarrays with primary tumours and a subset of paired lymph node metastases from 175 patients operated with pancreaticoduodenectomy for periampullary adenocarcinoma. PMID: 25323550
expression of SATB1 may increase the size of the BCSC population via the activation of the Notch signaling pathway and by increasing expression levels of Snail1 and Twist1. PMID: 25586771
SATB1 and HER2 expression correlated with poorly differentiated breast cancer and indicated an unfavorable prognosis. PMID: 25956130
SATB1 is overexpressed in pancreatic cancer, promoting cancer cell proliferation and invasion through the activation of MYC. PMID: 26108419
Up-regulation of histidine-rich calcium binding protein promotes tumor metastasis in hepatocellular carcinoma and is mediated by SATB1. PMID: 25762622
results suggest that SATB1 plays a crucial role in the progression of bladder cancer by regulating genes controlling EMT processes PMID: 25706386
SATB1 mRNA, protein, and immunoreactivity levels did not correlate with patients' clinicopathological data and their overall survival, but the latter analysis was limited by a relatively short period of follow PMID: 25874491
our data provide compelling evidence that miR-23a functions as a tumor suppressor in osteosarcoma, and its inhibitory effect on tumor are mediated chiefly through downregulation of SATB1. PMID: 25619478
Expression of SATB1 promotes the growth and metastasis of colorectal cancer. PMID: 24971456
High SATB1 expression is associated with recurrence in rectal cancer. PMID: 25496125
Overexpression of special AT-rich sequence-binding protein 1 is associated with endometrial cancer. PMID: 25347096
Moderate and high expression of SATB1 correlate with significantly better prognosis of cutaneous T-cell lymphoma patients. PMID: 25384658
Data suggest that SATB1 expression is down-regulated during activation of hepatic stellate cell (as seen in liver fibrosis); up-regulation of SATB1 expression appears to reduce collagen synthesis and cell activation/proliferation/migration. PMID: 25896016
This review will discuss the four major findings regarding SATB1/2 in colorectal cancer studies.[review] PMID: 25543122
SATB1 is indispensable for lymphocyte differentiation and stem cell development. (Review) PMID: 24938377

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Subcellular Location

Nucleus matrix. Nucleus, PML body. Note=Organized into a cage-like network anchoring loops of heterochromatin and tethering specialized DNA sequences. When sumoylated, localized in promyelocytic leukemia nuclear bodies (PML NBs).

Protein Families

CUT homeobox family

Tissue Specificity

Expressed predominantly in thymus.

Database Links

HGNC: 10541

OMIM: 602075

KEGG: hsa:6304

UniGene: Hs.517717

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Code	CSB-RA834512A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: THP-1 whole cell lysate(30µg), Jurkat whole cell lysate(30µg) All lanes: SATB1 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 86 kDa Observed band size: 100 kDa Exposure time: 2min
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SATB1 Recombinant Monoclonal Antibody

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