Product Details

Uniprot No.

Target Names

Alternative Names

Solute carrier family 2, facilitated glucose transporter member 2 (Glucose transporter type 2, liver) (GLUT-2), SLC2A2, GLUT2

Species Reactivity

Human

Immunogen

A synthesized peptide derived from Human SLC2A2

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

37E1

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

SLC2A2, commonly known as GLUT2, serves as a critical low-affinity glucose transporter predominantly expressed in hepatocytes, pancreatic beta cells, and intestinal epithelial cells. This transporter plays an essential role in glucose sensing and homeostasis, functioning as a bidirectional glucose transporter that responds to fluctuating blood glucose levels. Its involvement in metabolic regulation makes it a significant target for researchers investigating diabetes, metabolic syndrome, and hepatocellular carcinoma, where altered glucose metabolism is a hallmark feature.

This recombinant monoclonal antibody, generated against a synthetic peptide derived from human SLC2A2, offers the consistency and reproducibility that demanding experimental workflows require. As a sequence-defined recombinant clone, it eliminates the lot-to-lot variability inherent in traditional hybridoma-derived antibodies, ensuring that your optimization efforts translate reliably across experiments and projects.

Validation studies demonstrate robust performance across multiple detection platforms. Immunohistochemistry on paraffin-embedded human liver cancer tissue reveals clear target detection using standard citrate buffer antigen retrieval, with effective staining at dilutions between 1:50 and 1:200. The antibody also performs well in cell-based applications, showing specific membrane and cytoplasmic staining in HepG2 hepatocellular carcinoma cells by immunofluorescence. Flow cytometry analysis of HepG2 cells confirms surface detection with clear separation from isotype control, supporting studies examining GLUT2 expression dynamics in live cell populations.

Whether you are investigating glucose transporter expression in cancer metabolism, exploring developmental biology of metabolic tissues, or characterizing stem cell differentiation, this antibody provides a reliable tool for detecting SLC2A2 in human samples across immunohistochemical, immunofluorescence, and flow cytometric applications.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Facilitative hexose transporter that mediates the transport of glucose and fructose. Likely mediates the bidirectional transfer of glucose across the plasma membrane of hepatocytes and is responsible for uptake of glucose by the beta cells; may comprise part of the glucose-sensing mechanism of the beta cell. May also participate with the Na(+)/glucose cotransporter in the transcellular transport of glucose in the small intestine and kidney. Also able to mediate the transport of dehydroascorbate.

Gene References into Functions

Data suggest that the following genetic modifications are involved in neonatal diabetes mellitus patients in Oman: (1) mutation in KCNJ11 (potassium voltage-gated channel subfamily J member 11; one patient); (2) mutation in GCK (glucokinase); (3) mutation in SLC2A2 (glucose transporter type 2); (4) chromosome 6q24 methylation abnormalities. PMID: 29329106
Results showed the glucose transporter GLUT2 was highly expressed in the lumen of sweat glands from atopic dermatitis (AD) patients. AD patients with chronic inflammation had significantly increased GLUT2 mRNA expression and near normal sweat glucose levels. PMID: 29677207
Data suggest expression of SGLT1 is markedly increased in kidney of patients with type 2 diabetes as compared to control subjects; SGLT1 mRNA is highly and significantly correlated with fasting and postprandial plasma glucose and HbA1c. In contrast, data suggest SGLT2 and GLUT2 mRNA in kidney are down-regulated in type 2 diabetes, but not to statistically significant level. (SGLT = sodium-glucose co-transporter) PMID: 28477418
The mutant tumors exhibited impaired proliferation, anoikis resistance, and migratory capability and had reduced adenylate energy charge. Further investigations also revealed that cANGPTL4 regulated the expression of Glut2 PMID: 28641978
Single nucleotide polymorphism in SLC2A2 gene is associated with glycemic response to metformin in Type 2 diabetes. PMID: 27500523
no significant associations between GLUT2 and/or TAS1R2 polymorphisms and fillings were found, but allele frequencies of the TAS1R2 variant were marginally significantly different between children with DMFT = 0 and DMFT >/=1. no significant interaction between both genes and risk of dental caries was found. GLUT2 and TASR1 polymorphisms may influence the risk of caries in the Czech population PMID: 26112465
Three novel variants and seven single-nucleotide polymorphisms associated with the myelomeningocele phenotype. PMID: 25776730
Homozygous splice-site mutation IVS8+5G>C (c.1068+5 G>C) of SLC2A2 was found in patient A and homozygous nonsense mutation c.1194T>A (p.Tyr398X) in patient B. Patient C harboured a missense mutation c.380C>A (p.Ala127Asp) PMID: 25919556
Data identified the last enzyme of the de novo purine synthesis pathway 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase (ATIC)and the putative tyrosine phosphatase PTPLAD1 as new regulators of Glut2(SLC2A2)translocation in HEK293 cells. SiRNA-mediated knockdown of ATIC delayed insulin response of Glut2 translocation while depletion of PTPLAD1(HACD3} strongly enhanced it in HEK293 cells. PMID: 25687571
A novel 6 nucleotide deletion in GLUT2 gene, a member of the facilitative glucose transporter family, is shown to be segregated with Fanconi-Bickel syndrome in an Iranian family. PMID: 25523092
SGLT1 or GLUT2 interact with the cytoskeleton in the intestinal epithelium during hexose absorption. PMID: 25711084
Mutations in the GLUT2 gene is associated with ccute metabolic acidosis in Fanconi-Bickel syndrome. PMID: 25165176
GLUT-2 expression may be associated with cholangiocarcinogenesis of large bile duct and is a helpful marker for detecting high-grade biliary intraepithelial neoplasia lesions in atypical bile ducts. PMID: 24824030
SGLT1 mRNA and GLUT2 mRNA expression are reduced significantly in CACo-2 cells exposed to berry extracts. PMID: 24236070
the first gain of function mutations for hGLUT2, revealing the importance of its receptor versus transporter function in pancreatic beta cell development and insulin secretion. PMID: 23986439
associated with caries risk PMID: 23257979
This study determined if single nucleotide polymorphisms in genes involved in fructose transport,SLC2A2 and SLC2A5 and metabolism, etohexokinase affect inter-individual variability in metabolic phenotypes. PMID: 23341889
Intestinal dehydroascorbic acid (DHA) transport is mediated by the facilitative sugar transporters, GLUT2 and GLUT8 PMID: 23396969
Genetic variant SLC2A2 is marginally associated with risk of cardiovascular disease in type 2 diabetes mellitus patients. PMID: 23185617
Mutation analysis of the GLUT2 gene in three unrelated Egyptian families with Fanconi-Bickel syndrome detected three different mutations. PMID: 22350464
Case-control analyses revealed a unique association between the G allele of rs9875793 and bipolar disorder patients with 'negative mood delusions' compared with controls. PMID: 23010768
GLUT2 gene expression is suppressed in Hepatitis C virus infection via downregulation of HNF-1alpha expression at transcriptional and posttranslational levels. PMID: 22993150
The finding that patients with homozygous SLC2A2 mutations can have neonatal diabetes supports a role for GLUT2 in the human beta cell. PMID: 22660720
Homozygous mutations in GLUT2, which cause Fanconi-Bickel syndrome, can lead to very different clinical and biochemical findings that are not limited to mild proximal renal tubulopathy but can include significant hypercalciuria. PMID: 22865906
report on two siblings with Fanconi-Bickel syndrome (FBS) and an unusually mild clinical course; both patients were found to be compound heterozygous for the novel GLUT2 (SLC2A2) mutations c.457_462delCTTATA (p.153_4delLI) and c.1250C>G (p.P417R) PMID: 22214819
Constitutive expression of GLUT2 in the apical membrane along with additional translocation of cytoplasmic GLUT2 to the apical membrane via an intact cytoskeleton and activated PKC appears responsible for enhanced carrier-mediated glucose uptake. PMID: 21943636
We report the first Chinese cases of Fanconi-Bickel syndrome (FBS),a rare inherited disease caused by mutations in the glucose transporter 2 gene, SLC2A2. PMID: 22145468
In human enterocytes, GLUT2 was consistently located in basolateral membranes; mice on a low-carbohydrate/high-fat diet for 12 months also exhibited endosomal GLUT2 accumulation and reduced glucose absorption. PMID: 21852673
Polyphenols, phenolic acids and tannins from strawberry and apple are potent inhibitors of GLUT2 and SGLT1 at concentrations predicted after dietary ingestion. PMID: 20564476
prostate cancer was inversely associated with the SLC2A2 rs5400 Thr110 allele PMID: 20142250
Intestinal glucose absorption by the apical GLUT2 pathway can be 3 to 5-times greater then by SGLT1 et the high concentration of sugar. PMID: 20201351
Genetic polymorphisms of SLC2A2 and HP is associated with serum cholesterol levels. PMID: 20066028
mutated in patients with fanconi-Bickel syndrome PMID: 11810292
Hepatocyte nuclear factor-1alpha recruits the transcriptional co-activator p300 on the GLUT2 gene promoter. PMID: 11978637
polymorphisms at positions -269, -44, or + 103 may affect GLUT2 gene transcription, possibly associated with reduced expression of the GLUT2 gene in NIDDM patients. PMID: 12017192
Expression is responsible for resistance to alloxan and streptozotocin toxicity. PMID: 14614558
We have found GLUT-2 and glucokinase mRNAs in several brain regions, including the ventromedial and arcuate nuclei of the hypothalamus PMID: 15009676
SNPs of SLC2A2 predict the conversion to diabetes in obese subjects with impaired glucose tolerance. PMID: 15983230
identify Glut2 as a GroPIns transporter in mammals, and define a physiologically relevant cell-permeation mechanism PMID: 17141226
kidney of diabetic rats, an initial and transient upregulation of GLUT2 was induced specifically by insulin only. PMID: 17204838
recent progress in elucidating the transcriptional regulation of GLUT2 in the liver and pancreatic beta-cells and the relevance to type 2 diabetes.[RREVIEW] PMID: 18220613
Data show that glucose transport in human airway epithelial cells in vitro and in vivo utilises GLUT2 transporters, and suggest that these transporters could contribute to glucose uptake/homeostasis in the human airway. PMID: 18239936
Our findings show that a genetic variation in GLUT2 is associated with habitual consumption of sugars, suggesting an underlying glucose-sensing mechanism that regulates food intake. PMID: 18349384
The contribution of GLUT2 to human metabolic diseases (Review) PMID: 19223655
combined presence of rs5393 & rs5394 polymorphisms of GLUT2 was more frequent in type 2 diabetics than non-diabeteics; rs5394 appeared to be associated with decreased glucose stimulated insulin release & a tendency to a reduced GLUT2 gene expression PMID: 19269875
The expression pattern of GLUT2 is reported in newly diagnosed esophageal adenocarcinoma by means of immunohistochemistry. PMID: 19554504

Hide All

Involvement in disease

Fanconi-Bickel syndrome (FBS)

Subcellular Location

Cell membrane; Multi-pass membrane protein.

Protein Families

Major facilitator superfamily, Sugar transporter (TC 2.A.1.1) family, Glucose transporter subfamily

Tissue Specificity

Liver, insulin-producing beta cell, small intestine and kidney.

Database Links

HGNC: 11006

OMIM: 138160

KEGG: hsa:6514

STRING: 9606.ENSP00000323568

UniGene: Hs.167584

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Code	CSB-RA438622A0HU
Size	US$210
	Order now
Image	IHC image of CSB-RA438622A0HU diluted at 1:50 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.20% DAB. Immunofluorescence staining of HepG2 with CSB-RA438622A0HU at 1:25, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 495-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing HepG2 cells surface stained with CSB-RA438622A0HU (red line) at 1:50. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1µg/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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SLC2A2 Recombinant Monoclonal Antibody

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