Code | CSB-RA592917A0HU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
WB | 1:500-1:5000 |
IHC | 1:50-1:200 |
IF | 1:20-1:200 |
The SOX10 recombinant monoclonal antibody is developed using protein technology and DNA recombinant technology. The process involves immunizing mice with a synthesized peptide derived from human SOX10 and then extracting the total RNA of spleen cells from the mice under aseptic conditions after a certain period of time. The cDNA is synthesized by RNA reverse transcription and used as a template for PCR amplification of the SOX10 antibody gene. The resulting gene is inserted into a vector and transfected into host cells for culture. The SOX10 recombinant monoclonal antibody is then purified from the cell culture supernatant using affinity chromatography and rigorously verified. This antibody can be used for detecting human SOX10 protein in ELISA, WB, IHC, and IF experiments.
The SOX10 protein is a transcription factor that plays an important role in the development of the neural crest, which gives rise to various cell types including neurons, glia, and melanocytes. SOX10 is also involved in the maintenance and differentiation of neural crest-derived cells in adults, particularly in the peripheral nervous system. Mutations in the SOX10 gene are associated with various genetic disorders, such as Waardenburg syndrome and Hirschsprung's disease.
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