Product Details

Uniprot No.

Target Names

SPRY4

Alternative Names

Protein sprouty homolog 4 antibody; Sprouty 4 antibody; Sprouty homolog 4 antibody; sprouty homolog 4 (Drosophila) antibody; SPRY 4 antibody; Spry-4 antibody; Spry4 antibody; SPY4_HUMAN antibody

Species Reactivity

Human

Immunogen

A synthesized peptide from human SPRY4 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

15E10

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

SPRY4 (Sprouty homolog 4) functions as a critical negative regulator of receptor tyrosine kinase signaling, particularly within the MAPK/ERK pathway. By modulating growth factor responses, SPRY4 plays essential roles in developmental processes, cellular proliferation, and has emerged as a significant focus in cancer research where its expression patterns may influence tumor progression and therapeutic responses.

This recombinant monoclonal antibody, generated from clone 15E10 in rabbit host, offers researchers the reproducibility advantages inherent to recombinant technology. Because the antibody sequence is defined and produced through controlled expression systems, you can expect consistent performance across experiments and between lots—an important consideration for longitudinal studies or when establishing standardized protocols in your laboratory.

Validation studies confirm robust performance across multiple experimental platforms. In Western blot applications, the antibody detects SPRY4 at the expected 33 kDa molecular weight, with successful detection demonstrated in A375, A431, HT29, and Colo205 whole cell lysates at 1:1000 dilution. This panel of human cancer cell lines provides confidence for researchers working with melanoma, epidermoid carcinoma, and colorectal adenocarcinoma models. For flow cytometry applications, the antibody has been validated using fixed and permeabilized A431 cells, showing clear positive signal separation from isotype control, making it suitable for intracellular protein quantification studies.

The antibody is supplied in a glycerol-containing buffer optimized for long-term storage stability and is compatible with ELISA workflows as well. For investigators exploring RTK signaling dynamics, growth factor pathway regulation, or SPRY4's tumor suppressor functions, this reagent provides a reliable tool for protein detection and quantification across complementary experimental approaches.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Suppresses the insulin receptor and EGFR-transduced MAPK signaling pathway, but does not inhibit MAPK activation by a constitutively active mutant Ras. Probably impairs the formation of GTP-Ras. Inhibits Ras-independent, but not Ras-dependent, activation of RAF1. Represses integrin-mediated cell spreading via inhibition of TESK1-mediated phosphorylation of cofilin.

Gene References into Functions

The findings indicate that SPRY4 and SPRY4-IT1 may act as oncogenes in testicular germ cell tumors via activation of the PI3K/Akt signaling pathway. PMID: 29410498
Results show that SPRY4 expression level was significantly decreased in glioma tissues, associated with short survival time, and a key target gene of miR-1908. PMID: 29048686
Studied BAK1, SPRY4 and GAB2 SNPs in pediatric germ cell tumors(GCT); found a variant in SPRY4 was associated with reduced risk of GCT; a variant in BAK1 was positively associated with GCT with a strong estimated effect for testis tumors; and a SNP in GAB2 was associated with increased risk for GCT. PMID: 28295819
results found that histone methylation mediated by CCAT1 could also contribute to the lower expression of SPRY4 in esophageal squamous cell carcinoma PMID: 27956498
mechanistic evidence that KSRP promotes the down-regulation of Spry4 by a previously unidentified mechanism, i.e. post-transcriptional mRNA regulation. PMID: 28275056
Polymorphisms of KITLG, SPRY4, and BAK1 genes in patients with testicular germ cell tumors and individuals with infertility associated with AZFc deletion of the Y chromosome PMID: 28064312
SPRY4 is involved in the development and progression of colorectal cancer. PMID: 27997895
Small interfering RNA (siRNA)-mediated knockdown of SPRY4 attenuated the AREG-induced down-regulation of E-cadherin. PMID: 26768617
These findings suggest that SPRY4-IT1 plays a direct role in the regulation of metastasis and progression of osteosarcoma. PMID: 26982001
Results identify the tumor suppressor SPRY4 as a novel molecular effector of MT1-MMP affecting melanoma cell motility. PMID: 26392417
Data show that proto-oncogene protein B-raf (BRAF) inhibition induces c-Jun N-terminal kinase (c-JUN) expression and c-JUN abundance and activation by down-regulating SPRY2/4 protein expression. PMID: 26286024
Specific protein 1 plays a crucial role in the regulation of Sprouty4 in response to serum. PMID: 25957915
SPRY4 is often deleted in secondary acute myeloid leukemia. PMID: 26204823
Sprouty 2 protein, but not Sprouty 4, is an independent prognostic biomarker for human epithelial ovarian cancer. PMID: 25630587
these results suggest the possibility that SPRY4-IT1 knockdown may induce apoptosis via lipin 2-mediated alterations in lipid metabolism leading to cellular lipotoxicity. PMID: 25344859
SPRY4 may function as a tumor suppressor in endometrial adenocarcinoma. PMID: 24811094
demonstrated that knockdown of SPRY4-IT1 reduced cell proliferation, invasiveness, and migration PMID: 24810925
Overexpression of Sprouty4 or pharmacological inhibition of ERK upregulated IL-1R1 expression in primary T cells.Sprouty4 and ERK play a critical role in developing iTh17 cells in Th17 cell-driven autoimmune diseases. PMID: 24732356
Sprouty4 expression negatively influences cell migration. PMID: 24402575
Overexpressed Spry4 in endothelial cells inhibited migration and vitronectin adhesion. Spry4 knockdown enhanced these. Overexpression decreased integrin beta3 protein post-transcriptionally by modulating its tyrosine phosphorylation by c-Src. PMID: 23955631
results validated previously reported genetic associations with testicular germ cell tumor in a Scandinavian population, and observed suggestive evidence of a parent-of-origin effect in SPRY4. PMID: 23640991
Sprouty2 (but not Sprouty4) has a role in inhibition of cell proliferation and migration of osteosarcoma cells PMID: 23831057
Spry1 and Spry4 have opposing roles in VSMC phenotypic modulation, and Spry1 maintains the VSMC differentiation phenotype in vitro in part through an Akt/FoxO/myocardin pathway. PMID: 23554919
siRNA-mediated knockdown of Spry1, Spry2, or Spry4 promotes IFN-inducible antileukemic effects in vitro and results in enhanced suppressive effects on malignant hematopoietic progenitors from patients with polycythemia vera. PMID: 23074222
The SPRY4 (rs4324715) variants were significantly associated with germ cell tumors only in the adolescent age group. PMID: 22072546
Spry4 is a downstream target of Wnt7A/Fzd 9 signaling and may have efficacy in the treatment of non-small cell lung cancer. PMID: 20501643
The Spry4 might be involved in the timely restriction of MAPK signals under hypoxic conditions, similar to its role in mitogen-regulated processes. PMID: 20482313
interacts with the dual specificity kinase TESK1; maps to 5q31.3 PMID: 12027893
Sprouty4 suppresses vascular epithelial growth factor (VEGF)-induced, Ras-independent activation of Raf1 but does not affect epidermal growth factor (EGF)-induced, Ras-dependent activation of Raf1. PMID: 12717443
Core promoter activity is located within proximal 0.4-kb region. Minimal ERK-inducible promoter activity is between -69 and -31. Core promoter region of hSpry4 gene exhibits significant homology to 5'-flanking region of mouse gene. PMID: 14977631
Sprouty4 expression is down regulated in human prostate cancer by DNA methylation and this decreased expression may contribute to increased cell migration. PMID: 16388505
Human, chimpanzee, rat and mouse SPRY4 orthologs, consisting of three exons, were well conserved PMID: 16465403
the Sprouty/Caveolin-1 interaction modulates signaling in a growth factor- and Sprouty isoform-specific manner PMID: 16877379
SPRY4 gene was found to be associated with schizophrenia. PMID: 18298822
These results demonstrate that common genetic variants affect TGCT risk and implicate KITLG and SPRY4 as genes involved in Testicular germ cell tumors susceptibility. PMID: 19483682
cell adaptation to activated FGFR3 include Sprouty4 activity, which silences the premature receptor signaling and suppress apoptosis. PMID: 19761767

Hide All

Involvement in disease

Hypogonadotropic hypogonadism 17 with or without anosmia (HH17)

Subcellular Location

Cytoplasm. Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side.

Protein Families

Sprouty family

Database Links

HGNC: 15533

OMIM: 607984

KEGG: hsa:81848

STRING: 9606.ENSP00000344967

UniGene: Hs.323308

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Code	CSB-RA284166A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: A375 whole cell lysate(30µg), A431 whole cell lysate(30µg), HT29 whole cell lysate(30µg), colo205 whole cell lysate(30µg) All lanes: Sprouty 4 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 33 kDa Observed band size: 33 kDa Exposure time：10s Overlay Peak curve showing A431 cells stained with CSB-RA284166A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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SPRY4 Recombinant Monoclonal Antibody

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Target Background